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Identification of genetic markers linked to anthracnose resistance in sorghum using association analysis

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Abstract

Anthracnose in sorghum caused by Colletotrichum sublineolum is one of the most destructive diseases affecting sorghum production under warm and humid conditions. Markers and genes linked to resistance to the disease are important for plant breeding. Using 14,739 SNP markers, we have mapped eight loci linked to resistance in sorghum through association analysis of a sorghum mini-core collection consisting of 242 diverse accessions evaluated for anthracnose resistance for 2 years in the field. The mini-core was representative of the International Crops Research Institute for the Semi-Arid Tropics’ world-wide sorghum landrace collection. Eight marker loci were associated with anthracnose resistance in both years. Except locus 8, disease resistance-related genes were found in all loci based on their physical distance from linked SNP markers. These include two NB-ARC class of R genes on chromosome 10 that were partially homologous to the rice blast resistance gene Pib, two hypersensitive response-related genes: autophagy-related protein 3 on chromosome 1 and 4 harpin-induced 1 (Hin1) homologs on chromosome 8, a RAV transcription factor that is also part of R gene pathway, an oxysterol-binding protein that functions in the non-specific host resistance, and homologs of menthone:neomenthol reductase (MNR) that catalyzes a menthone reduction to produce the antimicrobial neomenthol. These genes and markers may be developed into molecular tools for genetic improvement of anthracnose resistance in sorghum.

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Acknowledgments

This study was supported in part by the International Crops Research Institute for the Semi-Arid Tropics and the University of Louisiana at Lafayette. We thank Karl H. Hasenstein and two reviewers for suggestions to improve the manuscript.

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  1. International Crops Research Institute for the Semi Arid Tropics (ICRISAT), Patancheru, Hyderabad, 502 324, Andhra Pradesh, India

    Hari D. Upadhyaya, Rajan Sharma & Shivali Sharma

  2. Department of Biology, University of Louisiana at Lafayette, Lafayette, LA, 70504, USA

    Yi-Hong Wang

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  1. Hari D. Upadhyaya
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Correspondence to Yi-Hong Wang.

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Upadhyaya, H.D., Wang, YH., Sharma, R. et al. Identification of genetic markers linked to anthracnose resistance in sorghum using association analysis. Theor Appl Genet 126, 1649–1657 (2013). https://doi.org/10.1007/s00122-013-2081-1

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