Advertisement

Theoretical and Applied Genetics

, Volume 126, Issue 6, pp 1639–1647 | Cite as

Fast track genetic improvement of ascochyta blight resistance and double podding in chickpea by marker-assisted backcrossing

  • B. Taran
  • T. D. Warkentin
  • A. Vandenberg
Original Paper

Abstract

Ascochyta blight (AB) caused by the fungus Ascochyta rabiei Pass. Lab. is one of the major diseases of chickpea worldwide and a constraint to production in western Canada. The use of varieties with high levels of resistance is considered the most economical solution for long-term ascochyta blight management in chickpea. QTL for resistance to ascochyta blight have been identified in chickpea. The availability of molecular markers associated with QTL for ascochyta blight resistant and double podding provides an opportunity to apply marker-assisted backcrossing to introgress the traits into adapted chickpea cultivars. In the present study, molecular markers that were linked to the QTL for ascochyta blight resistance and the double podding trait, and those unlinked to the resistance were used in foreground and background selection, respectively, in backcrosses between moderately resistant donors (CDC Frontier and CDC 425-14) and the adapted varieties (CDC Xena, CDC Leader and FLIP98-135C). The strategy included two backcrosses and selection for two QTL for ascochyta blight resistance and a locus associated with double podding. The fixation of the elite genetic background was monitored with 16–22 SSR markers to accelerate restoration of the genetic background at each backcross. By the BC2F1 generation, plants with improved ascochyta blight resistance and double podding were identified. The selected plants possessed the majority of elite parental type SSR alleles on all fragments analyzed except the segment of LG 4, LG 6 and LG 8 that possessed the target QTL. The results showed that the adapted variety could be efficiently converted into a variety with improved resistance in two backcross generations.

Keywords

Cetyl Trimethyl Ammonium Bromide Ascochyta Blight Background Selection Recipient Parent Elite Parent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Parvaneh Hashemi and Carmen Breitkreutz for their technical assistance. Financial support from the Agricultural Development Fund of the Saskatchewan Ministry of Agriculture is gratefully acknowledged.

References

  1. Acikgoz N, Karaca M, Er C, Meyveci K (1994) Chickpea and lentil production in Turkey. In: Muehlbauer FJ, Kaiser WJ (eds) Expanding the production and use of cool season food legumes. Kluwer Academic Publ, Dordrecht, pp 388–398CrossRefGoogle Scholar
  2. Anbessa Y, Warkentin TD, Bueckert R, Vandenberg A (2007) Short internode, double podding and early flowering effects on maturity and other agronomic characters in chickpea. Field Crop Res 102:43–50CrossRefGoogle Scholar
  3. Anbessa Y, Taran B, Warkentin TD, Tullu A, Vandenberg A (2009) Genetic analyses and conservation of QTL for ascochyta blight resistance across different populations of chickpea (Cicer arietinum L.). Theor Appl Genet 119:757–765PubMedCrossRefGoogle Scholar
  4. Cho S, Chen W, Muehlbauer FJ (2004) Pathotype-specific genetic factors in chickpea (Cicer arietinum L.) for quantitative resistance to ascochyta blight. Theor Appl Genet 109:733–739PubMedCrossRefGoogle Scholar
  5. Doyle JJ, Doyle JL (1990) Isolation of plants DNA from fresh tissue. Focus 12:13–15Google Scholar
  6. Flandez-Galvez H, Ades PK, Ford R, Pang ECK, Taylor PWJ (2003) QTL analysis for ascochyta blight resistance in an intraspecific population of chickpea (Cicer arietinum L.). Theor Appl Genet 107:1257–1265PubMedCrossRefGoogle Scholar
  7. Food and Agriculture Organization of the United Nations, FAOSTAT database (FAOSTAT, 2011), available at http://faostat.fao.org/site/567/DesktopDefault.aspx?PageID=567#ancor
  8. Frisch M, Melchinger AE (2005) Selection theory for marker-assisted backcrossing. Genetics 170:909–917PubMedCrossRefGoogle Scholar
  9. Frisch M, Bohn M, Melchinger AE (1999a) Comparison of selection strategies for marker-assisted backcrossing of a gene. Crop Sci 39:1295–1301CrossRefGoogle Scholar
  10. Frisch M, Bohn M, Melchinger AE (1999b) Minimum sample size and optimal positioning of flanking markers in marker-assisted backcrossing for transfer of a target gene. Crop Sci 39:967–975CrossRefGoogle Scholar
  11. Garg R, Patel RK, Jhanwar S, Priya P, Bhattacharjee A, Yadav G, Bhatia S, Chattopadhyay D, Tyagi AK, Jain M (2011) Gene discovery and tissue-specific transcriptome analysis in chickpea with massively parallel pyrosequencing and web resource development. Plant Physiol 156:1661–1678PubMedCrossRefGoogle Scholar
  12. Gujaria N, Kumar A, Dauthal P, Dubey A, Hiremath P, Bhanu Prakash A, Farmer A, Bhide M, Shah T, Gaur PM, Upadhyaya HD, Bhatia S, Cook DR, May GD, Varshney RK (2011) Development and use of genic molecular markers (GMMs) for construction of a transcript map of chickpea (Cicer arietinum L.). Theor Appl Genet 122:1577–1589PubMedCrossRefGoogle Scholar
  13. Gupta PK, Varshney RK (2000) The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica 113:163–185CrossRefGoogle Scholar
  14. Hospital F (2001) Size of donor chromosome segments around introgressed loci and reduction of linkage drag in marker-assisted backcross programs. Genetics 158:1363–1379PubMedGoogle Scholar
  15. Hospital F (2003) Marker-assisted breeding. In: Newbury HJ (ed) Plant molecular breeding. Blackwell Publishing, Oxford, pp 30–59Google Scholar
  16. Hospital F, Charcosset A (1997) Marker-assisted introgression of quantitative trait loci. Genetics 147:1469–1485PubMedGoogle Scholar
  17. Hospital F, Chevalet C, Mulsant P (1992) Using markers in gene introgression breeding programs. Genetics 132:1199–1210PubMedGoogle Scholar
  18. Jimenez-Diaz RM, Crino P, Halila MH, Mosconi C, Trapero-Casas AT (1993) Screening for resistance to fusarium wilt and ascochyta blight in chickpea. In: Singh KB, Saxena MC (eds) Breeding for stress tolerance in cool-season food legumes. ICARDA, Aleppo, pp 77–95Google Scholar
  19. Knights EJ (1987) The double podded gene in chickpea improvement. Int Chickpea Newslett 17:6–7Google Scholar
  20. Kumar J, Shrivastava RK, Ganesh M (2000) Penetrance and expressivity of the gene for double podding in chickpea. J Hered 91:234–236PubMedCrossRefGoogle Scholar
  21. Lichtenzveig J, Bonfil DJ, Zhang HB, Shtienberg D, Abbo S (2006) Mapping quantitative trait loci in chickpea associated with time to flowering and resistance to Didymella rabiei the causal agent of Ascochyta blight. Theor Appl Genet 113:1357–1369PubMedCrossRefGoogle Scholar
  22. Millán T, Clarke HJ, Sidiqque KHM, Buhariwalla HK, Gaur PM, Kumar J, Gil J, Kahl G, Winter P (2006) Chickpea molecular breeding: new tools and concepts. Euphytica 147:81–103CrossRefGoogle Scholar
  23. Millán T, Winter P, Jungling R, Gil J, Rubio J, Cho S, Cobos MJ, Iruela M, Rajesh PN, Tekeoglu M, Kahl G, Muehlbauer FJ (2010) A consensus genetic map of chickpea (Cicer arietinum L.) based on 10 mapping populations. Euphytica 175:175–189CrossRefGoogle Scholar
  24. Nene YL (1984) A review of ascochyta blight of chickpea (Cicer arietinum L.). In: Saxena MC, Singh KB (eds) Ascochyta blight and winter sowing of chickpea. Martinus Nijhoff/Dr. W. Junk Publisher, The Hague, pp 17–34Google Scholar
  25. Radhika P, Gowda S, Kadoo N, Mhase L, Jamadagni B, Sainani M, Chandra S, Gupta V (2007) Development of an integrated intraspecific map of chickpea (Cicer arietinum L) using two recombinant inbred line populations. Theor Appl Genet 115:209–216PubMedCrossRefGoogle Scholar
  26. Rajesh PN, Tullu A, Gil J, Gupta VS, Ranjekar PK, Muehlbauer FJ (2002) Identification of an STMS marker for the double-podding gene in chickpea. Theor Appl Genet 105:604–607PubMedCrossRefGoogle Scholar
  27. Rubio J, Moreno MT, Cubero JI, Gil J (1998) Effect of the gene for double pod in chickpea on yield, yield components and stability of yield. Plant Breed 117:585–587CrossRefGoogle Scholar
  28. Servin B, Hospital F (2002) Optimal positioning of markers to control genetic background in marker-assisted backcrossing. J Hered 93:214–217PubMedCrossRefGoogle Scholar
  29. Servin B, Martin OC, Mezard M, Hospital F (2004) Toward a theory of marker-assisted gene pyramiding. Genetics 168:513–523PubMedCrossRefGoogle Scholar
  30. Sheldrake AR, Saxena NP, Krishnamurthy L (1978) The expression and influence on yield of the ‘double-podded’ character in chickpeas (Cicer arietinum L.). Field Crops Res 1:243–253CrossRefGoogle Scholar
  31. Singh KB, Reddy MV (1993) Resistance to six races of Ascochyta rabiei in the world germplasm collection of chickpea. Crop Sci 33:186–189CrossRefGoogle Scholar
  32. Singh KB, Reddy MV (1996) Improving chickpea yield by incorporating resistance to ascochyta blight. Theor Appl Genet 92:509–515CrossRefGoogle Scholar
  33. Singh O, van Rheenen HA (1989) A possible role for the double podded character in stabilizing the yield of chickpea. Indian J Pulses Res 2:97–101Google Scholar
  34. Singh O, van Rheenen HA (1994) Genetics and contribution of the multi seeded and double-podded character to grain yield of chickpea. Indian J Pulses Res 7:97–102Google Scholar
  35. Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Biotechnology 7:257–264CrossRefGoogle Scholar
  36. Tar’an B, Warkentin T, Tullu A, Vandenberg A (2007) Genetic mapping of ascochyta blight resistance in chickpea (Cicer arietinum L.) using an SSR linkage map. Genome 50:26–34PubMedCrossRefGoogle Scholar
  37. Tar’an B, Malhotra R, Warkentin T, Banniza S, Vandenberg A (2009) CDC Luna kabuli chickpea. Can J Plant Sci 89:517–518CrossRefGoogle Scholar
  38. Tar’an B, Bandara M, Warkentin T, Vandenberg A, Banniza S (2011) CDC Orion kabuli chickpea. Can J Plant Sci 91:355–356CrossRefGoogle Scholar
  39. Thudi M, Bohra A, Nayak SN, Varghese N, Shah TM, Penmetsa RV, Thirunavukkarasu N, Gudipati S, Gaur PM, Kulwal PL, Upadhyaya HD, Kavikishor PB, Winter P, Kahl G, Town CD, Kilian A, Cook DR, Varshney RK (2011) Novel SSR markers from BAC-end sequences, DArT arrays and a comprehensive genetic map with 1,291 marker loci for chickpea (Cicer arietinum L.). PLoS One 6:e27275PubMedCrossRefGoogle Scholar
  40. Udapa SM, Baum M (2003) Genetic dissection of pathotype-specific resistance to ascochyta blight disease in chickpea (Cicer arietinum L.) using microsatellite markers. Theor Appl Genet 106:1196–1202Google Scholar
  41. Visscher PM, Haley CS, Thompson R (1996) Marker-assisted introgression in backcross breeding programs. Genetics 144:1923–1932PubMedGoogle Scholar
  42. Warkentin TD, Bannniza S, Vandenberg A (2005) CDC Frontier kabuli chickpea. Can J Plant Sci 85:909–910CrossRefGoogle Scholar
  43. Young ND, Tanksley SD (1989) RFLP analysis of the size of chromosomal segments retained around the tm-2 locus of tomato during backcross breeding. Theor Appl Genet 77:353–359CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Crop Development CenterUniversity of SaskatchewanSaskatoonCanada

Personalised recommendations