Theoretical and Applied Genetics

, Volume 126, Issue 5, pp 1397–1403 | Cite as

Identification of the gene Pm47 on chromosome 7BS conferring resistance to powdery mildew in the Chinese wheat landrace Hongyanglazi

  • Minggang Xiao
  • Fengjing Song
  • Jianfeng Jiao
  • Xiaoming Wang
  • Hongxing Xu
  • Hongjie Li
Original Paper

Abstract

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is an important disease that causes substantial yield losses in wheat (Triticum aestivum) in China and other parts of the world. This foliar disease can be effectively managed by host resistance. The Chinese landrace Hongyanglazi from Shaanxi province is highly resistant to many Bgt isolates at the seedling stage. Genetic analysis using an F2:3 population derived from a cross between Hongyanglazi and susceptible cultivar Zhongzuo 9504 indicated that Hongyanglazi carried a single recessive gene (tentatively designated PmHYLZ) conferring its resistance to Bgt isolate E09. PmHYLZ was flanked by EST marker BE606897 and microsatellite marker Xgwm46 on chromosome 7BS at genetic distances of 1.7 and 3.6 cM, respectively. This gene differed from Pm40, also located on 7BS, by origin, linked markers, and reactions to 13 Bgt isolates. Based on these findings, PmHYLZ was permanently designated as Pm47.

References

  1. Bassam BJ, Anollés GC, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80–83PubMedCrossRefGoogle Scholar
  2. Bossolini E, Wicker T, Knobel PA, Keller B (2007) Comparison of orthologous loci from small grass genomes Brachypodium and rice: implications for wheat genomics and grass genome annotation. Plant J 49:704–717PubMedCrossRefGoogle Scholar
  3. Brenchley R, Spannagl M, Pfeifer M, Barker GA, D’Amore R, Allen AM, McKenzie N, Kramer M, Kerhornou A, Bolser D, Kay S, Waite D, Trick M, Bancroft I, Gu Y, Hou NX, Luo MC, Sehgal S, Gill B, Kianian S, Anderson O, Kersey P, Dovrak J, McCombie WR, Hall A, Mayer KFX, Edwards K, Bevan MW, Hall N (2012) Analysis of the bread wheat genome using whole-genome shotgun sequencing. Nature 491:705–710PubMedCrossRefGoogle Scholar
  4. Cao SQ, Luo HS, Wu CP, Jin SL, Jin MA, Jia QZ, Zhang B, Huang J, Wang XM (2010) Evaluation of 193 Gansu landraces on wheat to powdery mildew. Gansu Agric Sci Tech 5:8–10Google Scholar
  5. Everts KL, Leath S (1992) Effect of early season powdery mildew on development, survival, and yield contribution of tillers of winter wheat. Phytopathology 82:1273–1278CrossRefGoogle Scholar
  6. Feuillet C, Keller B (2004) Molecular markers for disease resistance: the example wheat. In: Lörz H, Wenzel G (eds) Molecular Marker Systems in Plant Breeding and Crop Improvement (Biotechnology in Agriculture and Forestry), vol 55. Springer, Berlin, pp 353–364CrossRefGoogle Scholar
  7. He ZH, Rajaram S, Xin ZY, Huang GZ (eds) (2001) A History of Wheat Breeding in China. CIMMYT, MexicoGoogle Scholar
  8. Hsam SLK, Zeller FJ (2002) Breeding for powdery mildew resistance in common wheat (Triticum aestivum L.). In: Belanger RR, Bushnell WR, Dik AJ, Carver TLW (eds) The Powdery Mildews, a Comprehensive Treatise. APS Press, St. Paul, MN, pp 219–238Google Scholar
  9. Hu WG, Wang YJ, Wang CY, Ji WQ (2007) Genetic analysis on the powdery mildew resistance of Shanxi wheat landraces. J Triticeae Crops 27:341–344Google Scholar
  10. Huang XQ, Röder MS (2004) Molecular mapping of powdery mildew resistance genes in wheat: a review. Euphytica 95:203–223CrossRefGoogle Scholar
  11. Huang XQ, Hsam SLK, Zeller FJ (1997) Identification of powdery mildew resistance genes in common wheat (Triticum aestivum L. em Thell.). IX. Cultivars, land races and breeding lines grown in China. Plant Breed 116:233–238CrossRefGoogle Scholar
  12. Huang XQ, Hsam SLK, Zeller FJ (2000a) Chromosomal location of powdery mildew resistance genes in Chinese wheat (Triticum aestivum L. em. Thell.) landraces Xiaobaidong and Fuzhuang 30. J Genet Breed 54:311–317Google Scholar
  13. Huang XQ, Hsam SLK, Zeller FJ, Wenzel G, Mohler V (2000b) Molecular mapping of the wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding. Theor Appl Genet 101:407–414CrossRefGoogle Scholar
  14. Huang XQ, Wang LX, Xu MX, Röder MS (2003) Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat (Triticum aestivum L.). Theor Appl Genet 106:858–865PubMedGoogle Scholar
  15. Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175Google Scholar
  16. Law CN, Wolfe MS (1966) Location of genetic factors for mildew resistance and ear emergence time on chromosome 7B of wheat. Can J Genet Cytol 8:462–470Google Scholar
  17. Li HJ, Wang XM, Song FJ, Wu CP, Wu XF, Zhang N, Zhou Y, Zhang XY (2011) Response to powdery mildew and detection of resistance genes in wheat cultivars from China. Acta Agron Sin 37:943–954CrossRefGoogle Scholar
  18. Limpert E, Andrivon D, Felsenstein FG (1988) Influence of different benzimidazole concentrations in agar medium on senescence of wheat leaf segments and on growth and sporulation of the wheat powdery mildew pathogen. J Plant Dis Protect 95:301–306Google Scholar
  19. Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with Mapmaker/EXP30 Whitehead Institute Techn Rep, 3rd edn. Whitehead Institute, CambridgeGoogle Scholar
  20. Luo PG, Luo HY, Chang ZJ, Zhang HY, Zhang M, Ren ZL (2009) Characterization and chromosomal location of Pm40 in common wheat: a new gene for resistance to powdery mildew derived from Elytrigia intermedium. Theor Appl Genet 118:1058–1064CrossRefGoogle Scholar
  21. Ma HQ, Kong ZX, Fu BS, Li N, Zhang LX, Jia HY, Ma ZQ (2011) Identification and mapping of a new powdery mildew resistance gene on chromosome 6D of common wheat. Theor Appl Genet 123:1099–1106PubMedCrossRefGoogle Scholar
  22. McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers WJ, Morris CF, Somers DJ, Appels R, Devos KM (2008) Catalogue of gene symbols for wheat: Proceedings of the 11th International Wheat Genetic Symp. Sydney University of Sydney Press, AustraliaGoogle Scholar
  23. McIntosh RA, Dubcovsky J, Rogers WJ, Morris CF, Appels R, Xia XC (2009) Catalogue of gene symbols for wheat: 2009 supplement. http://www.wheat.pw.usda.gov
  24. McIntosh RA, Dubcovsky J, Rogers WJ, Morris CF, Appels R, Xia XC (2011) Catalogue of gene symbols for wheat: 2011 supplement. http://www.wheat.pw.usda.gov
  25. Michelmore RW, Paran I, Kesseli VR (1991) Identification of markers closely linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832PubMedCrossRefGoogle Scholar
  26. Qin B, Cao AZ, Wang HY, Chen TT, You FM, Liu YY, Ji JH, Liu DJ, Chen PD, Wang XE (2011) Collinearity-based marker mining for the fine mapping of Pm6, a powdery mildew resistance gene in wheat. Theor Appl Genet 123:207–218PubMedCrossRefGoogle Scholar
  27. Schmolk M, Mohler V, Hartl L, Zeller FJ, Sai L, Hsam K (2012) A new powdery mildew resistance allele at the Pm4 wheat locus transferred from einkorn (Triticum monococcum). Mol Breed 29:449–456CrossRefGoogle Scholar
  28. Sharp PG, Kreis M, Shewry PR, Gale MD (1988) Location of β-amylase sequence in wheat and its relatives. Theor Appl Genet 75:289–290CrossRefGoogle Scholar
  29. Sheng BQ, Duan XY, Zhou YL, Wang JX (1992) Studies on the classification of some wheat landraces resistant to powdery mildew. Crop Germplasm Resour 4:33–35Google Scholar
  30. Song FJ, Xiao MG, Huang J, Wang XM, Zhu ZD, Wu XF, Li HJ (2012) Inheritance of resistance to powdery mildew in 12 wheat cultivars. Acta Agron Sin 38:1339–1345Google Scholar
  31. Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi LL, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genomics 4:12–25PubMedCrossRefGoogle Scholar
  32. Wang XF, Zhang ZS, Liu HY, He WL (1996) Evaluation of resistance and slow-mildewing of some wheat varieties on Henan Province. Acta Agric Univ Henanensis 30:160–164Google Scholar
  33. Xie CJ, Sun QX, Ni ZF, Yang T, Nevo E, Fahima T (2003) Chromosomal location of a Triticum dicoccoides-derived powdery mildew resistance gene in common wheat by using microsatellite markers. Theor Appl Genet 106:341–345PubMedGoogle Scholar
  34. Xiong EH, Zhu W, Cao Y, Cai SB, Fang XW (1995) A genetic analysis of powdery mildew resistance in three native wheat varieties. J Jiangsu Agric College 16:47–50Google Scholar
  35. Xue F, Zhai WW, Duan XY, Zhou YL, Ji WQ (2009) Microsatellite mapping of powdery mildew resistance gene in wheat landrace Xiaobaidong. Acta Agron Sin 34:1193–1198Google Scholar
  36. Xue F, Wang CY, Li C, Duan XY, Zhou YL, Zhao NJ, Wang YJ, Ji WQ (2012) Molecular mapping of a powdery mildew resistance gene in common wheat landrace Baihulu and its allelism with Pm24. Theor Appl Genet 125:1425–1432PubMedCrossRefGoogle Scholar
  37. Zhai WW, Duan XY, Zhou YL, Ma HQ (2008) Inheritance of resistance to powdery mildew in four Chinese landraces. Plant Protect 34:37–40CrossRefGoogle Scholar
  38. Zhou RH, Zhu ZD, Kong XY, Huo NX, Tian QZ, Li P, Jin CY, Dong YC, Jia JZ (2005) Development of wheat near-isogenic lines for powdery mildew resistance. Theor Appl Genet 110:640–648PubMedCrossRefGoogle Scholar
  39. Zhu YL, Wang LM, Wang HG (2008) Studies on SSR molecular marker of wheat powdery mildew resistance gene Pm5e. Mol Plant Breed 6:1080–1084Google Scholar
  40. Zhuang QS (2003) Wheat improvement and pedigree analysis in China. China Agriculture Press, BeijingGoogle Scholar
  41. Zhuang QS, Li ZS (1993) Present status of wheat breeding and related genetic study in China. Wheat Inform Serv 76:1–15Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Minggang Xiao
    • 1
  • Fengjing Song
    • 1
  • Jianfeng Jiao
    • 2
  • Xiaoming Wang
    • 1
  • Hongxing Xu
    • 3
  • Hongjie Li
    • 1
  1. 1.The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural SciencesBeijingChina
  2. 2.Forestry College of Henan University of Science and TechnologyLuoyangChina
  3. 3.Center for Agricultural Resources ResearchInstitute of Genetics and Developmental Biology, Chinese Academy of SciencesShijiazhuangChina

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