Theoretical and Applied Genetics

, Volume 126, Issue 5, pp 1227–1236 | Cite as

Nucleotide sequence variation of GLABRA1 contributing to phenotypic variation of leaf hairiness in Brassicaceae vegetables

  • Feng Li
  • Zhongwei Zou
  • Hui-Yee Yong
  • Hiroyasu Kitashiba
  • Takeshi Nishio
Original Paper


GLABRA1 (GL1) belongs to the group of R2R3-MYB transcription factors and is known to be essential for trichome initiation in Arabidopsis. In our previous study, we identified a GL1 ortholog in Brassica rapa as a candidate for the gene controlling leaf hairiness by QTL analysis and suggested that a 5-bp deletion (B-allele) and a 2-bp deletion (D-allele) in the exon 3 of BrGL1 and a non-synonymous SNP (C-allele) in the second nucleotide of exon 3 possibly cause leaf hairlessness. In this study, we transformed a B. rapa line having the B-allele with the A-allele (wild type) or the C-allele of BrGL1 under the control of the CaMV 35S promoter. The transgenic plants with the A-allele showed dense coverage of seedling tissues including stems, young leaves and hypocotyls with trichomes, whereas the phenotypes of those with the C-allele were unchanged. In order to obtain more information about allelic variation of GL1 in different plant lineages and its correlation with leaf hairiness, two GL1 homologs, i.e., RsGL1a and RsGL1b, in Raphanus sativus were analyzed. Allelic variation of RsGL1a between a hairless line and a hairy line was completely associated with hairiness in their BC1F1 population. Comparison of the full-length of RsGL1a in the hairless and hairy lines showed great variation of nucleotides in the 3′ end, which might be essential for its function and expression.


Transgenic Plant Shoot Apical Meristem Trichome Density BC1F1 Population Nucleotide Sequence Variation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We are grateful to Dr. Ishida of NARO Institute of Vegetable and Tea Science and Dr. Sakamoto of Takii Seed Co. Ltd. for providing plant materials of R. sativus. This work was supported in part by the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN).

Supplementary material

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Feng Li
    • 1
    • 2
  • Zhongwei Zou
    • 1
  • Hui-Yee Yong
    • 1
  • Hiroyasu Kitashiba
    • 1
  • Takeshi Nishio
    • 1
  1. 1.Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural ScienceTohoku UniversitySendaiJapan
  2. 2.Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesWuhanChina

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