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Development and characterization of a compensating wheat-Thinopyrum intermedium Robertsonian translocation with Sr44 resistance to stem rust (Ug99)

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Abstract

The emergence of the highly virulent Ug99 race complex of the stem rust fungus (Puccinia graminis Pers. f. sp. tritici Eriks. and Henn.) threatens wheat (Triticum aestivum L.) production worldwide. One of the effective genes against the Ug99 race complex is Sr44, which was derived from Thinopyrum intermedium (Host) Barkworth and D.R. Dewey and mapped to the short arm of 7J (designated 7J#1S) present in the noncompensating T7DS-7J#1L∙7J#1S translocation. Noncompensating wheat-alien translocations are known to cause genomic duplications and deficiencies leading to poor agronomic performance, precluding their direct use in wheat improvement. The present study was initiated to produce compensating wheat-Th. intermedium Robertsonian translocations with Sr44 resistance. One compensating RobT was identified consisting of the wheat 7DL arm translocated to the Th. intermedium 7J#1S arm resulting in T7DL∙7J#1S. The T7DL∙7J#1S stock was designated as TA5657. The 7DL∙7J#1S stock carries Sr44 and has resistance to the Ug99 race complex. This compensating RobT with Sr44 resistance may be useful in wheat improvement. In addition, we identified an unnamed stem rust resistance gene located on the 7J#1L arm that confers resistance not only to Ug99, but also to race TRTTF, which is virulent to Sr44. However, the action of the second gene can be modified by the presence of suppressors in the recipient wheat cultivars.

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Acknowledgments

This research was part of the project “Durable Rust Resistance in Wheat” conducted by Cornell University and supported by the Bill and Melinda Gates Foundation and by grants from the Kansas Wheat Commission and the Kansas Crop Improvement Association. We thank W. John Raupp for critical editorial review of the manuscript and Shuangye Wu for her technical assistance. This is contribution number 12-451-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, KS 66506-5502, USA. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer.

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  1. Wenxuan Liu

    Present address: Laboratory of Cell and Chromosome Engineering, College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan, 450002, People’s Republic of China

  2. Michael O. Pumphrey

    Present address: Spring Wheat Breeding and Genetics, Department of Crop and Soil Sciences, Washington State University, 291D Johnson Hall, Pullman, WA, 99164-6420, USA

Authors and Affiliations

  1. Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA

    Wenxuan Liu, Tatiana V. Danilova, Bernd Friebe & Bikram S. Gill

  2. USDA-ARS Cereal Disease Laboratory, University of Minnesota, St. Paul, MN, 55108, USA

    Matthew N. Rouse

  3. USDA-ARS, Hard Winter Wheat Genetics Research Unit, Kansas State University, Throckmorton Plant Sciences Center, Manhattan, KS, 66506-5502, USA

    Robert L. Bowden & Michael O. Pumphrey

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  1. Wenxuan Liu
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  2. Tatiana V. Danilova
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Correspondence to Bernd Friebe.

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Communicated by P. Heslop-Harrison.

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Liu, W., Danilova, T.V., Rouse, M.N. et al. Development and characterization of a compensating wheat-Thinopyrum intermedium Robertsonian translocation with Sr44 resistance to stem rust (Ug99). Theor Appl Genet 126, 1167–1177 (2013). https://doi.org/10.1007/s00122-013-2044-6

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