Theoretical and Applied Genetics

, Volume 125, Issue 7, pp 1403–1411 | Cite as

Inheritance and molecular mapping of a gene conferring seedling resistance against Puccinia hordei in the barley cultivar Ricardo

  • K. S. Sandhu
  • K. L. Forrest
  • S. Kong
  • U. K. Bansal
  • D. Singh
  • M. J. Hayden
  • R. F. ParkEmail author
Original Paper


Genetic studies were undertaken to determine the inheritance and genomic location of uncharacterised seedling resistance to leaf rust, caused by Puccinia hordei, in the barley cultivar Ricardo. The resistance was shown to be conferred by a single dominant gene, which was tentatively designated RphRic. Bulk segregant analysis (BSA) and genetic mapping of an F3 mapping population using multiplex-ready SSR genotyping and Illumina GoldenGate SNP assay located RphRic in chromosome 4H. Given that this is the first gene for leaf rust resistance mapped on chromosome 4H, it was designated Rph21. The presence of an additional gene, Rph2, in Ricardo, was confirmed by the test of allelism. The seedling gene Rph21 has shown effectiveness against all Australian pathotypes of P. hordei tested since at least 1992 and hence represents a new and useful source of resistance to this pathogen.


Leaf Rust Rust Resistance Rust Resistance Gene Adult Plant Resistance Bulk Segregant Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



K. S. Sandhu would like to sincerely thank and acknowledge the Australian Grains Research and Development Corporation for the provision of a Postgraduate Research Scholarship and the support provided by the University of Sydney that enabled these studies to be initiated and completed. Valuable technical support provided by Mr Matthew Williams is also acknowledged.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • K. S. Sandhu
    • 1
    • 2
  • K. L. Forrest
    • 3
  • S. Kong
    • 3
  • U. K. Bansal
    • 1
  • D. Singh
    • 1
  • M. J. Hayden
    • 3
  • R. F. Park
    • 1
    Email author
  1. 1.Plant Breeding InstituteThe University of SydneyNarellanAustralia
  2. 2.Department of Agriculture, Fisheries and Forestry, Crop and Food Science, Agri-Science QueenslandLeslie Research CentreToowoombaAustralia
  3. 3.Department of Primary IndustriesVictorian AgriBioscience CentreBundooraAustralia

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