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Identification of markers linked to the race Ug99 effective stem rust resistance gene Sr28 in wheat (Triticum aestivum L.)

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Abstract

Wheat stem rust caused by Puccinia graminis f. sp. tritici can cause devastating yield losses in wheat. Over the past several decades, stem rust has been controlled worldwide through the use of genetic resistance. Stem rust race TTKSK (Ug99), first detected in Uganda in 1998, threatens global wheat production because of its unique virulence combination. As the majority of the currently grown cultivars and advanced breeding lines are susceptible to race TTKSK, sources of resistance need to be identified and characterized to facilitate their use in agriculture. South Dakota breeding line SD 1691 displayed resistance to race TTKSK in the international wheat stem rust nursery in Njoro, Kenya. Seedling screening of progeny derived from SD 1691 crossed to susceptible LMPG-6 indicated that a single resistance gene was present. Allelism and race-specificity tests indicated the stem rust resistance gene in SD 1691 was Sr28. The chromosome arm location of Sr28 was previously demonstrated to be 2BL. We identified molecular markers linked to Sr28 and validated this linkage in two additional populations. Common spring wheat cultivars in the central United States displayed allelic diversity for markers flanking Sr28. These markers could be used to select for Sr28 in breeding populations and for combining Sr28 with other stem rust resistance genes.

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Acknowledgments

Funding for this research was provided by USDA-ARS, USAID, and the Durable Rust Resistance in Wheat project (Bill and Melinda Gates Foundation). The research would not have been completed without assistance from Sarah Nelson, Sam Gale, Lucy Wanschura, Jennifer Flor, Mary Osenga, and several University of Minnesota undergraduate student technicians. We acknowledge the Computational Genetics Laboratory of the University of Minnesota Supercomputing Institute for computational support. Mention of trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the USDA, and does not imply its approval to the exclusion of other products and vendors that might also be suitable.

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Authors and Affiliations

  1. United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory, 1551 Lindig Street, St. Paul, MN, USA

    Matthew N. Rouse & Yue Jin

  2. Department of Plant Pathology, University of Minnesota, 1991 Upper Buford Circle, 495 Borlaug Hall, St. Paul, MN, 55108, USA

    Matthew N. Rouse & Yue Jin

  3. Department of Agronomy and Plant Genetics, University of Minnesota, 1991 Upper Buford Circle, 411 Borlaug Hall, St. Paul, MN, 55108, USA

    Itamar C. Nava & James A. Anderson

  4. USDA-ARS, Biosciences Research Laboratory, 1605 Albrecht Blvd. N., Fargo, ND, 58102, USA

    Shiaoman Chao

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  1. Matthew N. Rouse
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Correspondence to Matthew N. Rouse.

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Communicated by F. Ordon.

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Rouse, M.N., Nava, I.C., Chao, S. et al. Identification of markers linked to the race Ug99 effective stem rust resistance gene Sr28 in wheat (Triticum aestivum L.). Theor Appl Genet 125, 877–885 (2012). https://doi.org/10.1007/s00122-012-1879-6

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