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Mapping of quantitative trait loci for canopy-wilting trait in soybean (Glycine max L. Merr)

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Abstract

Drought stress adversely affects [Glycine max (L.) Merr] soybean at most developmental stages, which collectively results in yield reduction. Little information is available on relative contribution and chromosomal locations of quantitative trait loci (QTL) conditioning drought tolerance in soybean. A Japanese germplasm accession, PI 416937, was found to possess drought resistance. Under moisture-deficit conditions, PI 416937 wilted more slowly in the field than elite cultivars and has been used as a parent in breeding programs to improve soybean productivity. A recombinant inbred line (RIL) population was derived from a cross between PI 416937 and Benning, and the population was phenotyped for canopy wilting under rain-fed field conditions in five distinct environments to identify the QTL associated with the canopy-wilting trait. In a combined analysis over environments, seven QTL that explained 75 % of the variation in canopy-wilting trait were identified on different chromosomes, implying the complexity of this trait. Five QTL inherited their positive alleles from PI 416937. Surprisingly, the other two QTL inherited their positive alleles from Benning. These putative QTL were co-localized with other QTL previously identified as related to plant abiotic stresses in soybean, suggesting that canopy-wilting QTL may be associated with additional morpho-physiological traits in soybean. A locus on chromosome 12 (Gm12) from PI 416937 was detected in the combined analysis as well as in each individual environment, and explained 27 % of the variation in canopy-wilting. QTL identified in PI 416937 could provide an efficient means to augment field-oriented development of drought-tolerant soybean cultivars.

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Acknowledgments

This research was supported by funds allocated to the Georgia Agricultural Experiment Stations and grants from the United Soybean Board. Appreciation is extended to Jonathan McCoy for technical assistance at the Stuttgart location, AR.

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Author notes

  1. Landon L. Ries

    Present address: Department of Agronomy and Plant Genetics, University of Minnesota, 1991 Upper Buford Circle, 411 Borlaug Hall, St. Paul, MN, 55108, USA

Authors and Affiliations

  1. Institute for Plant Breeding, Genetics and Genomics, Center for Applied Genetic Technologies, University of Georgia, 111 Riverbend Rd., Athens, GA, 30602-6810, USA

    Hussein Abdel-Haleem & H. Roger Boerma

  2. USDA-ARS at North Carolina State University, Raleigh, NC, 27607, USA

    Thomas E. Carter Jr. & Thomas R. Sinclair

  3. Department of Crops, Soils, and Environmental Sciences, University of Arkansas, 1336 Altheimer Drive, Fayetteville, AR, 72704, USA

    Larry C. Purcell, C. Andy King & Landon L. Ries

  4. Department of Crops, Soils, and Environmental Sciences, University of Arkansas, 115 Plant Science Bldg., Fayetteville, AR, 72701, USA

    Pengyin Chen

  5. Department of Agronomy, Kansas State University, 2004C Throckmorton Hall, Manhattan, KS, 66506-5501, USA

    William Schapaugh Jr.

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  1. Hussein Abdel-Haleem
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Correspondence to Hussein Abdel-Haleem.

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Communicated by H. T. Nguyen.

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Abdel-Haleem, H., Carter, T.E., Purcell, L.C. et al. Mapping of quantitative trait loci for canopy-wilting trait in soybean (Glycine max L. Merr). Theor Appl Genet 125, 837–846 (2012). https://doi.org/10.1007/s00122-012-1876-9

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  • DOI: https://doi.org/10.1007/s00122-012-1876-9

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