Theoretical and Applied Genetics

, Volume 125, Issue 3, pp 609–623 | Cite as

Fine mapping, phenotypic characterization and validation of non-race-specific resistance to powdery mildew in a wheat–Triticum militinae introgression line

  • Irena Jakobson
  • Diana Reis
  • Anu Tiidema
  • Hilma Peusha
  • Ljudmilla Timofejeva
  • Miroslav Valárik
  • Monika Kladivová
  • Hana Šimková
  • Jaroslav Doležel
  • Kadri Järve
Original Paper

Abstract

Introgression of several genomic loci from tetraploid Triticum militinae into bread wheat cv. Tähti has increased resistance of introgression line 8.1 to powdery mildew in seedlings and adult plants. In our previous work, only a major quantitative trait locus (QTL) on chromosome 4AL of the line 8.1 contributed significantly to resistance, whereas QTL on chromosomes 1A, 1B, 2A, 5A and 5B were detected merely on a suggestive level. To verify and characterize all QTLs in the line 8.1, a mapping population of double haploid lines was established. Testing for seedling resistance to 16 different races/mixtures of Blumeria graminis f. sp. tritici revealed four highly significant non-race-specific resistance QTL including the main QTL on chromosome 4AL, and a race-specific QTL on chromosome 5B. The major QTL on chromosome 4AL (QPm.tut-4A) as well as QTL on chromosome 5AL and a newly detected QTL on 7AL were highly effective at the adult stage. The QPm.tut-4A QTL accounts on average for 33–49 % of the variation in resistance in the double haploid population. Interactions between the main QTL QPm.tut-4A and the minor QTL were evaluated and discussed. A population of 98 F2 plants from a cross of susceptible cv. Chinese Spring and the line 8.1 was created that allowed mapping the QPm.tut-4A locus to the proximal 2.5-cM region of the introgressed segment on chromosome 4AL. The results obtained in this work make it feasible to use QPm.tut-4A in resistance breeding and provide a solid basis for positional cloning of the major QTL.

Supplementary material

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Supplementary material 1 (PDF 579 kb)
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Supplementary material 2 (DOC 92 kb)
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Supplementary material 3 (DOC 125 kb)
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Supplementary material 4 (PDF 502 kb)
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Supplementary material 5 (DOC 41 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Irena Jakobson
    • 1
  • Diana Reis
    • 1
  • Anu Tiidema
    • 1
  • Hilma Peusha
    • 1
  • Ljudmilla Timofejeva
    • 1
  • Miroslav Valárik
    • 2
  • Monika Kladivová
    • 2
  • Hana Šimková
    • 2
  • Jaroslav Doležel
    • 2
  • Kadri Järve
    • 1
  1. 1.Department of Gene TechnologyTallinn University of TechnologyTallinnEstonia
  2. 2.Centre of the Region Haná for Biotechnological and Agricultural ResearchInstitute of Experimental BotanyOlomoucCzech Republic

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