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Theoretical and Applied Genetics

, Volume 124, Issue 7, pp 1325–1338 | Cite as

SSR-based genetic maps of Miscanthus sinensis and M. sacchariflorus, and their comparison to sorghum

  • Changsoo Kim
  • Dong Zhang
  • Susan A. Auckland
  • Lisa K. Rainville
  • Katrin Jakob
  • Brent Kronmiller
  • Erik J. Sacks
  • Martin Deuter
  • Andrew H. PatersonEmail author
Original Paper

Abstract

We present SSR-based genetic maps from a cross between Miscanthus sacchariflorus Robustus and M. sinensis, the progenitors of the promising cellulosic biofuel feedstock Miscanthus × giganteus. cDNA-derived SSR markers were mapped by the two-way pseudo-testcross model due to the high heterozygosity of each parental species. A total of 261 loci were mapped in M. sacchariflorus, spanning 40 linkage groups and 1,998.8 cM, covering an estimated 72.7% of the genome. For M. sinensis, a total of 303 loci were mapped, forming 23 linkage groups and 2,238.3 cM, covering 84.9% of the genome. The use of cDNA-derived SSR loci permitted alignment of the Miscanthus linkage groups to the sorghum chromosomes, revealing a whole genome duplication affecting the Miscanthus lineage after the divergence of subtribes Sorghinae and Saccharinae, as well as traces of the pan-cereal whole genome duplication. While the present maps provide for many early research needs in this emerging crop, additional markers are also needed to improve map density and to further characterize the structural changes of the Miscanthus genome since its divergence from sorghum and Saccharum.

Keywords

Sorghum Sugarcane Basal Chromosome Number Sorghum Genome Sorghum Chromosome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by the US Department of Energy-US Department of Agriculture Plant Feedstock Program (project grant number: 112786), the Consortium for Plant Biotechnology Research (CPBR), and Mendel Biotechnology.

Supplementary material

122_2012_1790_MOESM1_ESM.xls (3.3 mb)
Supplementary material 1 (XLS 3392 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Changsoo Kim
    • 1
  • Dong Zhang
    • 1
  • Susan A. Auckland
    • 1
  • Lisa K. Rainville
    • 1
  • Katrin Jakob
    • 2
  • Brent Kronmiller
    • 2
  • Erik J. Sacks
    • 3
  • Martin Deuter
    • 4
  • Andrew H. Paterson
    • 1
    Email author
  1. 1.Plant Genome Mapping LaboratoryUniversity of GeorgiaAthensUSA
  2. 2.Mendel BiotechnologyHaywardUSA
  3. 3.Department of Crop SciencesUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  4. 4.TinplantKlein WanzlebenGermany

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