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Identification and comparative mapping of a powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides) on chromosome 2BS

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Abstract

Powdery mildew, caused by Blumeria graminis f. sp. tritici, is an important foliar disease of wheat worldwide. Wild emmer (Triticum turgidum var. dicoccoides) is a valuable genetic resource for improving disease resistance in common wheat. A powdery mildew resistance gene conferring resistance to B. graminis f. sp. tritici isolate E09 at the seedling and adult stages was identified in wild emmer accession IW170 introduced from Israel. An incomplete dominant gene, temporarily designated MlIW170, was responsible for the resistance. Through molecular marker and bulked segregant analyses of an F2 population and F3 families derived from a cross between susceptible durum wheat line 81086A and IW170, MlIW170 was located in the distal chromosome bin 2BS3-0.84-1.00 and flanked by SSR markers Xcfd238 and Xwmc243. MlIW170 co-segregated with Xcau516, an STS marker developed from RFLP marker Xwg516 that co-segregated with powdery mildew resistance gene Pm26 on 2BS. Four EST–STS markers, BE498358, BF201235, BQ160080, and BF146221, were integrated into the genetic linkage map of MlIW170. Three AFLP markers, XPaacMcac, XPagcMcta, XPaacMcag, and seven AFLP-derived SCAR markers, XcauG2, XcauG3, XcauG6, XcauG8, XcauG10, XcauG20, and XcauG25, were linked to MlIW170. XcauG3, a resistance gene analog (RGA)-like sequence, co-segregated with MlIW170. The non-glaucousness locus Iw1 was 18.77 cM distal to MlIW170. By comparative genomics of wheat–Brachypodium–rice genomic co-linearity, four EST–STS markers, CJ658408, CJ945509, BQ169830, CJ945085, and one STS marker XP2430, were developed and MlIW170 was mapped in an 2.69 cM interval that is co-linear with a 131 kb genomic region in Brachypodium and a 105 kb genomic region in rice. Four RGA-like sequences annotated in the orthologous Brachypodium genomic region could serve as chromosome landing target regions for map-based cloning of MlIW170.

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Acknowledgments

The authors are grateful to Melinda Moore for improving the manuscript. This work was financially supported by the National Science Foundation for Distinguished Young Scholars (30425039), National Natural Science Foundation of China (30771341, 30971780, 31030056), 863 project 2011AA100104, MOA projects 2008ZX08009 and 2009ZX08009-048B, the Program of Introducing Talents of Discipline to Universities (111-2-03), the Program for Changjiang Scholars and Innovative Research Teams in Universities and Innovation Fund for Graduate Student of China Agricultural University. The aneuploid and deletion stocks as gifts from Prof. B.S. Gill and Mr. W.J. Raupp, Wheat Genetics Resource Centre, Kansas State University, USA, are gratefully acknowledged.

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Correspondence to Zhiyong Liu.

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Communicated by P. Langridge.

Z. Liu and J. Zhu contributed equally to this work.

The seed stock of IW170 and its derivatives are available upon request to Zhiyong Liu, China Agricultural University at zhiyongliu@cau.edu.cn.

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Liu, Z., Zhu, J., Cui, Y. et al. Identification and comparative mapping of a powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides) on chromosome 2BS. Theor Appl Genet 124, 1041–1049 (2012). https://doi.org/10.1007/s00122-011-1767-5

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  • DOI: https://doi.org/10.1007/s00122-011-1767-5

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