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Theoretical and Applied Genetics

, Volume 124, Issue 4, pp 777–788 | Cite as

Introgression and pyramiding into common bean market class fabada of genes conferring resistance to anthracnose and potyvirus

  • Juan José FerreiraEmail author
  • Ana Campa
  • Elena Pérez-Vega
  • Cristina Rodríguez-Suárez
  • Ramón Giraldez
Original Paper

Abstract

Anthracnose and bean common mosaic (BCM) are considered major diseases in common bean crop causing severe yield losses worldwide. This work describes the introgression and pyramiding of genes conferring genetic resistance to BCM and anthracnose local races into line A25, a bean genotype classified as market class fabada. Resistant plants were selected using resistance tests or combining resistance tests and marker-assisted selection. Lines A252, A321, A493, Sanilac BC6-Are, and BRB130 were used as resistance sources. Resistance genes to anthracnose (Co-2 C , Co-2 A252 and Co-3/9) and/or BCM (I and bc-3) were introgressed in line A25 through six parallel backcrossing programs, and six breeding lines showing a fabada seed phenotype were obtained after six backcross generations: line A1258 from A252; A1231 from A321; A1220 from A493; A1183 and A1878 from Sanilac BC6-Are; and line A2418 from BRB130. Pyramiding of different genes were developed using the pedigree method from a single cross between lines obtained in the introgression step: line A1699 (derived from cross A1258 × A1220), A2438 (A1220 × A1183), A2806 (A1878 × A2418), and A3308 (A1699 × A2806). A characterization based on eight morpho-agronomic traits revealed a limited differentiation among the obtained breeding lines and the recurrent line A25. However, using a set of seven molecular markers linked to the loci used in the breeding programs it was possible to differentiate the 11 fabada lines. Considering the genetic control of the resistance in resistant donor lines, the observed segregations in the last backcrossing generation, the reaction against the pathogens, and the expression of the molecular markers it was also possible to infer the genotype conferring resistance in the ten fabada breeding lines obtained. As a result of these breeding programs, genetic resistance to three anthracnose races controlled by genes included in clusters Co-2 and Co-3/9, and genetic resistance to BCM controlled by genotype I + bc-3 was combined in the fabada line A3308.

Keywords

Common Bean Recurrent Parent Backcross Generation Donor Line Bean Common Mosaic Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by grants RTA02-052-C02-01 and RTA2005-00115-C02-01 from INIA-Ministerio de Ciencia y Tecnología, Spain. Elena Pérez-Vega was recipient of a salary fellowship from the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA, Spain). Lines A252, A321, A493 and BRB130 were originally supplied by S.P. Singh (Kimberly Research and Extension, University of Idaho). Line Sanilac BC6-Are was supplied by A de Ron (Misión Biológica de Galicia, Pontevedra, Spain).

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Juan José Ferreira
    • 1
    Email author
  • Ana Campa
    • 1
  • Elena Pérez-Vega
    • 1
  • Cristina Rodríguez-Suárez
    • 2
  • Ramón Giraldez
    • 3
  1. 1.Área de Cultivos Hortofrutícolas y Forestales, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA)VillaviciosaSpain
  2. 2.Instituto de Agricultura SostenibleCSICCórdobaSpain
  3. 3.Department of Biología FuncionalUniversity of OviedoOviedoSpain

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