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Analysis of gene expression profiles of two near-isogenic lines differing at a QTL region affecting oil content at high temperatures during seed maturation in oilseed rape (Brassica napus L.)

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Abstract

Seed oil production in oilseed rape is greatly affected by the temperature during seed maturation. However, the molecular mechanism of the interaction between genotype and temperature in seed maturation remains largely unknown. We developed two near-isogenic lines (NIL-9 and NIL-1), differing mainly at a QTL region influencing oil content on Brassica napus chromosome C2 (qOC.C2.2) under high temperature during seed maturation. The NILs were treated under different temperatures in a growth chamber after flowering. RNA from developing seeds was extracted on the 25th day after flowering (DAF), and transcriptomes were determined by microarray analysis. Statistical analysis indicated that genotype, temperature, and the interaction between genotype and temperature (G × T) all significantly affected the expression of the genes in the 25 DAF seeds, resulting in 4,982, 19,111, and 839 differentially expressed unisequences, respectively. NIL-9 had higher seed oil content than NIL-1 under all of the temperatures in the experiments, especially at high temperatures. A total of 39 genes, among which six are located at qOC.C2.2, were differentially expressed among the NILs regardless of temperature, indicating the core genetic divergence that was unaffected by temperature. Increasing the temperature caused a reduction in seed oil content that was accompanied by the downregulation of a number of genes associated with red light response, photosynthesis, response to gibberellic acid stimulus, and translational elongation, as well as several genes of importance in the lipid metabolism pathway. These results contribute to our knowledge of the molecular nature of QTLs and the interaction between genotype and temperature.

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Acknowledgments

The work of LJ lab was supported by Natural Science Foundation of China (30971700 and 31171463) and of Zhejiang Province (Z3100130), Special Grand National Science and Technology Project (2009ZX08009-076B). Work at the JZ lab was supported in part by the National Research Council Canada-Genomic and Health Initiative Program. The authors are indebted to Dr. Faouzi Bekkaoui and Dr. Wenyun Shen for project management and technical support, to Dr. Jianyi Zhao of Zhejiang Academic of Agricultural Sciences for providing a part of the molecular markers for genotyping and sequence alignment.

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  1. College of Agriculture and Biotechnology, Zhejiang University, 866 Yu-Hang-Tang Road, Hangzhou, 310058, People’s Republic of China

    Yana Zhu, Fei Xu, Mingxun Chen, Wanli Guo, Weijun Zhou, Jun Zhu & Lixi Jiang

  2. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Zhengying Cao & Jinling Meng

  3. Plant Biotechnology Institute, National Research Council Canada, 110 Gymnasium Place, Saskatoon, SK, S7N 0W9, Canada

    Yi Huang & Jitao Zou

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  1. Yana Zhu
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Correspondence to Jinling Meng, Jitao Zou or Lixi Jiang.

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Communicated by R. Visser.

Y. Zhu and Z. Cao contributed equally to this paper.

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Zhu, Y., Cao, Z., Xu, F. et al. Analysis of gene expression profiles of two near-isogenic lines differing at a QTL region affecting oil content at high temperatures during seed maturation in oilseed rape (Brassica napus L.). Theor Appl Genet 124, 515–531 (2012). https://doi.org/10.1007/s00122-011-1725-2

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