High resolution mapping of Dense spike-ar (dsp.ar) to the genetic centromere of barley chromosome 7H
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Spike density in barley is under the control of several major genes, as documented previously by genetic analysis of a number of morphological mutants. One such class of mutants affects the rachis internode length leading to dense or compact spikes and the underlying genes were designated dense spike (dsp). We previously delimited two introgressed genomic segments on chromosome 3H (21 SNP loci, 35.5 cM) and 7H (17 SNP loci, 20.34 cM) in BW265, a BC7F3 nearly isogenic line (NIL) of cv. Bowman as potentially containing the dense spike mutant locus dsp.ar, by genotyping 1,536 single nucleotide polymorphism (SNP) markers in both BW265 and its recurrent parent. Here, the gene was allocated by high-resolution bi-parental mapping to a 0.37 cM interval between markers SC57808 (Hv_SPL14)–CAPSK06413 residing on the short and long arm at the genetic centromere of chromosome 7H, respectively. This region putatively contains more than 800 genes as deduced by comparison with the collinear regions of barley, rice, sorghum and Brachypodium, Classical map-based isolation of the gene dsp.ar thus will be complicated due to the infavorable relationship of genetic to physical distances at the target locus.
KeywordsSorghum Cleave Amplify Polymorphic Sequence Internode Length Brachypodium Cleave Amplify Polymorphic Sequence Marker
We gratefully acknowledge Mary Ziems, Corine Graser, Naser Poursarebani, Matthias Jost and Nikki Bonar for excellent technical assistance. The project was supported in frame of the ERA-PG project BARCODE by grants of SFC (Scotland), DFG (Germany) and MUR (Italy) to RW, NS and MM, respectively. The groups of RW, MM and NS collaborate for cereal mutant research in frame of COST action FA0604 Tritigen. We thank David Harrap of KWS-UK Ltd for making the mutant crosses employed here.
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