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Simple sequence repeat markers linked to QTL for resistance to Watermelon mosaic virus in melon

Theoretical and Applied Genetics volume 123, pages 1207–1214 (2011)Cite this article

Abstract

A population of recombinant inbred lines (RIL) derived from a cross between the Watermelon mosaic virus (WMV) resistant genotype TGR-1551 and the susceptible Spanish cultivar ‘Bola de Oro’ has been evaluated for WMV resistance in spring, fall and growth chamber conditions. The quantitative trait loci (QTL) analyses detected one major QTL (wmv) on linkage group (LG) XI close to the microsatellite marker CMN04_35. This QTL controls the resistance to WMV in the three environmental conditions evaluated. Other minor QTLs affecting the severity of viral symptoms were identified, but they were not detected in all the assayed environments. The screening of the marker CMN04_35 in an F2 progeny, derived from the same cross, confirmed the effect of this QTL on the expression of WMV resistance also in early generations, which evidences the usefulness of this marker for a marker assisted selection program.

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References

  • Abou-Jawdah Y, Sobh H, El-Zammar S, Fayyad A, Lecoq H (2000) Incidence and management of virus diseases of cucurbits in Lebanon. Crop Protection 19:217–224

    Article  Google Scholar 

  • Ali A, Natsuaki T, Okuda S (2006) The complete nucleotide sequence of a Pakistani isolate of Watermelon mosaic virus provides further insights into the taxonomic status in the Bean common mosaic virus subgroup. Virus Genes 32:307–311

    Article  PubMed  CAS  Google Scholar 

  • Brotman Y, Kovalski I, Dogimont C, Pitrat M, Portnoy V, Kaztir N, Perl-Treves R (2005) Molecular markers linked to papaya ring spot virus resistance and Fusarium race 2 resistance in melon. Theor Appl Genet 110:337–345

    Article  PubMed  CAS  Google Scholar 

  • Castle SJ, Perring TM, Farrar CA, Kishaba AN (1992) Field and laboratory transmission of watermelon mosaic virus 2 and zucchini yellow mosaic virus by various aphid species. Phytopathol 82:235–240

    Article  Google Scholar 

  • Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for detection of plant viruses. J Gen Virol 34:475–483

    Article  PubMed  CAS  Google Scholar 

  • Collard BCY, Jahufer MZZ, Brower JB, Pang ECK (2005) An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: the basic concepts. Euphytica 142:169–196

    Article  CAS  Google Scholar 

  • Daning-Poleg Y, Reis N, Baudracco-Arnas S, Pitrat M, Staub JE, Oliver M, Arús P, de Vicente CM, Katzir N (2001) Development and characterization of microsatellite markers in Cucumis. Theor Appl Genet 102:61–72

    Article  Google Scholar 

  • Daning-Poleg Y, Tadmor Y, Tzuri G, Reis N, Hirschberg J, Katzir N (2002) Construction of a genetic map of melon with molecular markers and horticultural traits, and localization of genes associated with ZYMV resistance. Euphytica 125:373–384

    Article  Google Scholar 

  • Desbiez C, Joannon B, Wipf-Scheibel C, Chandeysson C, Lecoq H (2009) Emergence of new strains of Watermelon mosaic virus in South-eastern France: evidence for limited spread but rapid local population shift. Virus Res 141:201–208

    Article  PubMed  CAS  Google Scholar 

  • Díaz JA, Mallor C, Soria C, Camero R, Garzo E, Fereres A, Alvarez JM, Gómez-Guillamón ML, Luis-Arteaga M, Moriones E (2003) Potential sources of resistance for melon to non-persistently aphid-borne viruses. Plant Dis 87:960–964

    Article  Google Scholar 

  • Díaz-Pendón JA, Fernández-Muñoz R, Gómez-Guillamón ML, Moriones E (2005) Inheritance of resistance to Watermelon mosaic virus in Cucumis melo that impairs virus accumulation, symptom expression, and aphid transmission. Phytopathol 95:840–846

    Article  Google Scholar 

  • Dogimont C, Bendahmane A, Pitrat M, Burget-Bigeard E, Hagen L, Le Menn A, Pauquet J, Rouselle P, Caboche M, Chovelon V (2007) Gene resistant to Aphis gossypii. US Patent Application US 2007/0016977 A1

  • Essafi A, Díaz-Pendón JA, Moriones E, Monforte AJ, García-Mas J, Martín-Hernández AM (2009) Dissection of the oligogenic resistance to Cucumber mosaic virus in the melon accession PI 161375. Theor Appl Genet 118:275–284

    Article  PubMed  CAS  Google Scholar 

  • Fazio G, Staub JE, Chung SM (2002) Development and characterization of PCR markers in cucumber (Cucumis sativus L.). J Am Soc Hortic Sci 127:545–557

    CAS  Google Scholar 

  • Fernández-Silva I, Eduardo I, Blanca J, Esteras C, Picó B, Nuez F, Arús P, García-Mas J, Monforte A (2008) Bin mapping of genomic and EST-derived SSRs in melon (Cucumis melo L.). Theor Appl Genet 118:139–150

    Article  PubMed  Google Scholar 

  • Fraser RSS (1990) The genetics of resistance to plant viruses. Annu Rev Phytopathol 9:275–276

    Google Scholar 

  • Fukino N, Sakata Y, Kunihisa M, Matsumoto S (2007) Characterization of novel simple sequence repeat (SSR) markers for melon (Cucumis melo L.) and their use for genotype identification. J Hort Sci Biotechnol 82:330–334 Details about primers sequences: http://cse.naro.affrc.go.jp/nbk/List_CMN.xls

  • Gilbert RZ, Kyle MM, Munger HM, Gray SM (1994) Inheritance of resistance to watermelon mosaic virus in Cucumis melo L. HortScience 29:107–110

    Google Scholar 

  • Gómez P, Rodríguez-Hernández AM, Moury B, Aranda MA (2009) Genetic resistance for the sustainable control of plant virus diseases: breeding, mechanisms and durability. Eur J Plant Pathol 125:1–22

    Article  Google Scholar 

  • Gonzalo MJ, Oliver M, García-Mas J, Monfort A, Dolcet-Sanjuan R, Katzir N, Arús P, Monforte AJ (2005) Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.). Theor Appl Genet 110:802–811

    Article  PubMed  CAS  Google Scholar 

  • Grafton-Cardwell EE, Perring TM, Smith RF, Valencia J, Farrar CA (1996) Occurrence of mosaic viruses in melons in central valley of California. Plant Dis 80:1092–1097

    Article  Google Scholar 

  • Gray SM, Moyer JW, Kennedy GG, Campbell CL (1986) Virus-suppression and aphid resistance effects on spatial and temporal spread of watermelon mosaic virus 2. Phytopathol 76:1254–1259

    Article  Google Scholar 

  • Hämäläinen JH, Watanabe KN, Valkonen JPT, Arihara A, Plaisted RL, Pehu E, Miller L, Slack SA (1997) Mapping and marker-assisted selection for a gene for extreme resistance to potato virus Y. Theor Appl Genet 94:192–197

    Article  Google Scholar 

  • Hooks CRR, Fereres A (2006) Protecting crops from non-persistently aphid-transmitted viruses: a review on the use of barrier plants as a management tool. Virus Res 120:1–16

    Article  PubMed  CAS  Google Scholar 

  • Jefferies SP, King BJ, Barr AR, Warner P, Logue SJ, Langridge P (2003) Marker-assisted backcross introgression of the Yd2 gene conferring resistance to barley yellow dwarf virus in barley. Plant Breed 122:52–56

    Article  CAS  Google Scholar 

  • Joobeur T, King JJ, Nolin SJ, Thomas CE, Dean RA (2004) Fusarium wilt resistance locus Fom-2 of melon contains a single resistance gene with complex features. Plant J 39:283–297

    Article  PubMed  CAS  Google Scholar 

  • Ko SJ, Lee YH, Cho MS, Park JW, Choi HS, Lim GC, Kim KH (2007) The incidence of virus diseases on melon in Jeonnam province during 2000–2002. Plant Pathol J 23:215–218

    Article  Google Scholar 

  • Kong Q, Xiang C, Yu Z, Zhang C, Liu F, Peng C, Peng X (2007) Mining and charactering microsatellites in Cucumis melo expressed sequence tags from sequence database. Mol Ecol Notes 7:281–283

    Article  CAS  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Annu Eugene 12:172–175

    Article  Google Scholar 

  • Lecoq H, Cohen S, Pitrat M, Labonne G (1979) Resistance to Cucumber Mosaic Virus transmission by aphids in Cucumis melo. Phytopathol 69:15–16

    Article  Google Scholar 

  • Lombaert E, Carletto J, Piotte C, Fauvergue X, Lecoq H, Vanberberghe-Masutti F, Lapchin L (2009) Response of the melon aphid, Aphis gossypii, to host-plant resistance: evidence for high adaptative potential despite low genetic variability. Entomol Exp Appl 133:46–56

    Article  Google Scholar 

  • López-Sesé AI, Staub JE, Katzir N, Gómez-Guillamón ML (2002) Estimation of between and within accession variation in selected Spanish melon germplasm using RAPD and SSR markers to assess strategies for large collection evaluation. Euphytica 127:41–51

    Article  Google Scholar 

  • Luis-Arteaga M, Alvarez JM, Alonso-Prados JL, Bernal JJ, García-Arenal F, Laviña A, Batlle A, Moriones E (1998) Occurrence, distribution, and relative incidence of mosaic virus infecting field-grown melon in Spain. Plant Dis 82:979–982

    Article  Google Scholar 

  • Mohan M, Nair S, Bhagwat A, Krishna TG, Yano M, Bhatia CR, Sasaki T (1997) Genome mapping, molecular markers and marker-assisted selection in crop plants. Mol Breed 3:87–103

    Article  CAS  Google Scholar 

  • Morales M, Orjeda G, Nieto C, van Leeuwen H, Monfort A, Charpentier M, Caboche M, Arús P, Puigdomènech P, Aranda MA, Dogimont C, Bendahmane A, García-Mas J (2005) A physical map covering the nsv locus that confers resistance to Melon necrotic spot virus in melon (Cucumis melo L.). Theor Appl Genet 111:914–922

    Article  PubMed  CAS  Google Scholar 

  • Munger HM (1991) Progress in breeding melons for watermelon mosaic resistance. Cucurbit Genet Coop Rpt 14:53–54

    Google Scholar 

  • Nameth S, Laemmlen F, Dodds JA (1985) Viruses cause heavy melon losses in desert valleys. Calif Agric 39:28–29

    Google Scholar 

  • Nieto C, Morales M, Gisella O, Clepet C, Monfort A, Sturbois B, Puigdomènech P, Pitrat M, Caboche M, Dogimont C, García-Mas J, Aranda MA, Bendahmane A (2006) An eIF4E allele confers resistance to an uncapped and non-poly-adenylated RNA virus in melon. Plant J 48:452–462

    Article  PubMed  CAS  Google Scholar 

  • Oumouloud A, Arnedo-Andrés MS, González-Torres R, Álvarez JM (2008) Development of molecular markers linked to the Fom-1 locus for resistance to Fusarium race 2 in melon. Euphytica 164:347–356

    Article  CAS  Google Scholar 

  • Perchepied L, Pitrat M (2004) Polygenic inheritance of partial resistance to Fusarium oxysporum f. sp. melonis race 1.2 in melon. Phytopathology 94:1331–1336

    Article  PubMed  CAS  Google Scholar 

  • Périn C, Hagen LS, de Conto V, Katzir N, Danin-Poleg Y, Portnoy V, Baudracco-Arnas S, Chadoeuf J, Dogimont C, Pitrat M (2002) A reference map of Cucumis melo based on two recombinant inbred line populations. Theor Appl Genet 104:1017–1034

    Article  PubMed  Google Scholar 

  • Purcifull D, Hiebert E, Edwardson J (1984) Watermelon mosaic virus 2. CMI/AAB descriptions of plant viruses no. 293 (no. 63 revised). http://www.dpvweb.net/dpv/showdpv.php?dpvno=293

  • Ritschel PS, Lins TCL, Tristan RL, Buso GSC, Buso JA, Ferreira ME (2004) Development of microsatellite markers from an enriched genomic library for genetic analysis of melon (Cucumis melo L.). BMC Plant Biol 4:9

    Article  PubMed  Google Scholar 

  • Robaglia C, Caranta C (2006) Translation initiation factors: a weak link in plant RNA virus infection. Trends Plant Sci 11:40–45

    Article  PubMed  CAS  Google Scholar 

  • Saghai Maroof MA, Jeong SC, Gunduz I, Tucker DM, Buss GR, Tolin SA (2008) Pyramiding of soybean mosaic virus resistance genes by marker-assisted selection. Crop Sci 48:517–526

    Article  Google Scholar 

  • Sauvion N, Mauriello V, Renard B, Boissot N (2005) Impact of melon accessions resistant to aphids on the demographic potential of silverleaf whitefly. J Econ Entomol 98:557–567

    Article  PubMed  Google Scholar 

  • Sevik MA, Arli-Sokmen M (2003) Viruses infecting cucurbits in Samsun, Turkey. Plant Dis 87:341–344

    Article  Google Scholar 

  • Shi A, Chen P, Li D, Zheng C, Zhang B, Hou A (2009) Pyramiding multiple genes for resistance to soybean mosaic virus in soybean using molecular markers. Mol Breed 23:113–114

    Article  CAS  Google Scholar 

  • Stapleton JJ, Summers CG (2002) Reflective mulches for management of aphids and aphid-borne virus diseases in late-season cantaloupe (Cucumis melo L. var. cantalupensis). Crop Protect 21:891–898

    Article  Google Scholar 

  • Sugiura N, Tsujii T, Fujii K, Kato T, Saka N, Tooyama T, Hayano Y, Toshihiko I (2004) Molecular marker-assisted selection in a recurrent backcross breeding for the incorporation of resistance to rice stripe virus and panicle blast in rice (Oryza sativa L.). Breed Res 6:143–148

    Article  Google Scholar 

  • van Leeuwen H, García-Mas J, Coca M, Puigdomenech P, Monfort A (2005) Analysis of the melon genome in regions encompassing TIR-NBS-LRR resistance genes. Mol Genet Genom 273:240–251

    Article  CAS  Google Scholar 

  • van Ooijen JW (2004) MapQTL® 5, Software for the mapping of quantitative trait loci in experimental populations. Kyazma B.V, Wageningen, Netherlands

    Google Scholar 

  • van Ooijen JW (2006) JoinMap® 4. Software for the calculation of genetic linkage maps in experimental populations. Kyazma B.V, Wageningen, Netherlands

    Google Scholar 

  • Wang YH, Thomas CE, Dean RA (2000) Genetic mapping of a Fusarium will resistance gene Fom-2 in melon (Cucumis melo L.). Mol Breed 6:379–389

    Article  CAS  Google Scholar 

  • Ward CW, Shukla DD (1991) Taxonomy of potyviruses: current problems and some solutions. Intervirol 32:269–296

    CAS  Google Scholar 

  • Yuki VA, Rezende JAM, Kitajima EW, Barroso PAV, Kuniyuki H, Groppo GA, Pavan MA (2000) Occurrence, distribution, and relative incidence of five viruses infecting Cucurbits in the state of São Paulo, Brazil. Plant Dis 84:516–520

    Article  Google Scholar 

  • Yuste-Lisbona FJ, Capel C, Gómez-Guillamón ML, Capel J, López-Sesé AI, Lozano R (2011) Codominant PCR-based markers and candidate genes for powdery mildew resistance in melon (Cucumis melo L.). Theor Appl Genet 122:747–758

    Article  PubMed  CAS  Google Scholar 

  • Zamir D, Ekstein-Michelson I, Zakay Y, Navot N, Zeidan M, Sarfatti M, Eshed Y, Harel E, Pleban T, van-Oss H, Kedar N, Rabinowitch HD, Czosnek H (1994) Mapping and introgression of a tomato yellow curl virus tolerance gene, TY-1. Theor Appl Genet 88:141–146

    Article  CAS  Google Scholar 

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Acknowledgments

The authors thank Dr. Díaz-Pendón for his useful support in the virus management and Laura Barrios, responsible for the Statistics Unit-CSIC for her help with the statistical analysis. This work has been financed by the CICYT Research Project: AGL2008-05687-C02-01 funded by the Spanish Ministerio de Ciencia e Innovación.

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  1. Fernando J. Yuste-Lisbona

    Present address: Departamento de Biología Aplicada (Genética), Edificio CITE II-B, Universidad de Almería, E-04120, Almería, Spain

Authors and Affiliations

  1. Instituto de Hortofruticultura Subtropical y Mediterránea ‘La Mayora’ (IHSM, UMA-CSIC), Algarrobo-Costa, E-29760, Málaga, Spain

    Francisco J. Palomares-Rius, Maria A. Viruel, Fernando J. Yuste-Lisbona, Ana I. López-Sesé & María L. Gómez-Guillamón

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  1. Francisco J. Palomares-Rius
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  2. Maria A. Viruel
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  3. Fernando J. Yuste-Lisbona
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  4. Ana I. López-Sesé
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  5. María L. Gómez-Guillamón
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Correspondence to María L. Gómez-Guillamón.

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Communicated by M. Havey.

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Palomares-Rius, F.J., Viruel, M.A., Yuste-Lisbona, F.J. et al. Simple sequence repeat markers linked to QTL for resistance to Watermelon mosaic virus in melon. Theor Appl Genet 123, 1207–1214 (2011). https://doi.org/10.1007/s00122-011-1660-2

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Keywords

  • Quantitative Trait Locus
  • Melon
  • Recombinant Inbred Line
  • Quantitative Trait Locus Analysis
  • Recombinant Inbred Line Population