With an aim to further characterize the cytoplasmic male sterility–fertility restoration system in sorghum, a major fertility restoration gene was mapped along with a second locus capable of partial restoration of pollen fertility. The major fertility restoration gene, Rf 5 , was located on sorghum chromosome SBI-05, and was capable of restoring pollen fertility in both A1 and A2 male sterile cytoplasms. Depending on the restorer parent, mapping populations exhibited fertility restoration phenotypes that ranged from nearly bimodal distribution due to the action of Rf 5 , to a more normalized distribution reflecting the action of Rf 5 and additional modifier/partial restoration genes. A second fertility restoration locus capable of partially restoring pollen fertility in A1 cytoplasm was localized to chromosome SBI-04. Unlike Rf 5 , this modifier/partial restorer gene acting alone resulted in less than 10% seed set in both A1 and A2 cytoplasms, and modified the extent of restoration conditioned by the major restorer Rf 5 in A1 cytoplasm. In examining the genomic regions spanning the Rf 5 locus, a cluster of pentatricopeptide gene family members with high homology to rice Rf 1 and sorghum Rf 2 were identified as potential candidates encoding Rf 5 .
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Ahnert D, Lee M, Austin DF, Livini C, Woodman WL, Openshaw SJ, Smith JSC, Porter K, Dalton G (1996) Genetic diversity among elite sorghum inbred lines assessed with DNA markers and pedigree information. Crop Sci 36:1385–1392
Bentolila S, Alfonso AA, Hanson MR (2002) A pentatricopeptide repeat-containing gene restores fertility to cytoplasmic male-sterile plants. Proc Natl Acad Sci USA 99:10887–10892. doi:101073/pnas102301599
Brown GG, Formanova N, Jin H, Wargachuk R, Dendy C, Patil P, Laforest M, Zhang JF, Cheung WY, Landry BS (2003) The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats. Plant J 35:262–272. doi:101046/j1365-313X200301799x
Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971
Cui XQ, Wise RP, Schnable PS (1996) The rf2 nuclear restorer gene of male-sterile T-cytoplasm maize. Science 272:1334–1336
Dahlberg JA, Madera-Torres P (1997) Restorer reaction in A1 (ATx623), A2 (A2Tx632), and A3 (A3SC103) cytoplasms to selected accessions from the Sudan sorghum collection. Int Sorghum Millet Newsl 38:43–58
Drummond A, Ashton B, Cheung M, Heled J, Kearse M, Moir R, Stones-Havas S, Thierer T, Wilson A (2009) Geneious v4.7. http://www.geneious.com/
Johnson M, Zaretskaya I, Raytselis Y, Merezhuk Y, McGinnis S, Madden TL (2008) NCBI BLAST: a better web interface. Nucl Acids Res 36:W5–W9. doi:101093/nar/gkn201
Jordan DR, Mace ES, Henzell RG, Klein PE, Klein RR (2010) Molecular mapping and candidate gene identification of the Rf2 gene for pollen fertility restoration in sorghum (Sorghum bicolor (L.) Moench). Theor Appl Genet 120(7):1279–1287
Kato H, Tezuka K, Feng YY, Kawamoto T, Takahashi H, Mori K, Akagi H (2007) Structural diversity and evolution of the Rf-1 locus in the genus Oryza. Heredity 99:516–524. doi:101038/sjhdy6801026
Klein RR, Klein PE, Mullet J, Minx P, Rooney WL, Schertz KF (2005) Fertility restorer locus Rf1 of sorghum (Sorghum bicolor L) encodes a pentatricopeptide repeat protein not present in the colinear region of rice chromosome 12. Theor Appl Genet 111:994–1012
Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits by using RFLP linkage maps. Genetics 136:1447–1455
Lurin C, Andres C, Aubourg S, Bellaoui M, Bitton F, Bruyere C, Caboche M, Debast C, Gualberto J, Hoffmann B, Lecharny A, Le Ret M, Matrin-Magnietee M-L, Mireau H, Peeters N, Renou J-P, Szurek B, Taconnat L, Small I (2004) Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins reveals their essential role in organelle biogenesis. Plant Cell 16:2089–2103
Mace ES, Jordan DR (2010) Location of major effect genes in sorghum [Sorghum bicolor (L.) Moench]. Theor Appl Genet 121:1339–1356
Mace ES, Xia L, Jordan DR, Halloran K, Parh DK, Huttner E, Wenzl P, Kilian A (2008) DArT markers: diversity analyses and mapping in Sorghum bicolor. BMC Genomics 9:26. doi:26101186/1471-2164-9-26
Mace ES, Rami JF, Bouchet S, Klein PE, Klein RR, Kilian A, Wenzl P, Xia L, Halloran K, Jordan DR (2009) A consensus genetic map of sorghum that integrates multiple component maps and high-throughput Diversity Array Technology (DArT) markers. BMC Plant Biol 9:13
Maunder AB, Pickett RC (1959) The genetic inheritance of cytoplasmic-genetic male sterility in grain sorghum. Agron J 51:47–49
Menz MA, Unruh NC, Klein PE, Mullet JE, Rooney WL, Klein RR (2004) Genetic diversity of public inbreds of sorghum determined by mapped AFLP and SSR markers. Crop Sci 44:1236–1244
Miller DA, Pickett RC (1964) Inheritance of partial male-fertility in Sorghum vulgare Pers. Crop Sci 4:1–4
Moran JL, Rooney WL (2003) Effect of cytoplasm on the agronomic performance of grain sorghum hybrids. Crop Sci 43:777–781
Parh DK, Jordan DR, Aitken EAB, Mace ES, Jun-ai P, McIntyre CL, Godwin ID (2008) QTL analysis of ergot resistance in sorghum. Theor Appl Genet 117:369–382
Reddy BVS, Ramesh S, Reddy PS, Ramaiah B (2007) Combining ability and heterosis as influenced by male-sterility inducing cytoplasms in sorghum [Sorghum bicolor (L.) Moench]. Euphytica 154:153–164. doi:101007/s10681-006-9281-6
Schertz KF (1983) Potentials with new cytoplasmic male sterility systems in sorghum. Proc Genet Soc USA 38:1–10
Schmitz-Linneweber C, Small I (2008) Pentatricopeptide repeat proteins: a socket set for organelle gene expression. Trends Plant Sci 13:663–670
Stephens JC, Holland RF (1954) Cytoplasmic male-sterility for hybrid sorghum seed production. Agron J 46:20–23
Tang HV, Chang RY, Pring DR (1998) Cosegregation of single genes associated with fertility restoration and transcript processing of sorghum mitochondrial orf107 and urf209. Genetics 150:383–391
Temnykh S, DeClerck G, Lukashova A, Lipovich L, Cartinhour S, McCouch S (2001) Computational and experimental analysis of microsatellites in rice (Oryza sativa L): frequency, length variation, transposon associations, and genetic marker potential. Genome Res 11:1441–1452
Ullstrup AJ (1972) The impacts of southern corn leaf blight epidemics of 1970–1971. Annu Rev Phytopathol 10:37–50
Wang S, Basten C, Zeng Z-B (2010) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh. http://statgenncsuedu/qtlcart/WQTLCarthtm
Xu XB, Liu ZX, Zhang DF, Liu Y, Song WB, Li JS, Dai JR (2009) Isolation and analysis of rice Rf1-orthologus PPR genes co-segregating with Rf3 in maize. Plant Mol Biol Rep 27:511–517. doi:101007/s11105-009-0105-4
The authors wish to thank Julie McCollum for her expert technical assistance. We thank the Australian Grains Research and Development Corporation (GRDC; http://www.grdc.com.au) for part of the financial support for this research, and the USDA-Agricultural Research Service for financial support to RRK.
Communicated by H. H. Geiger.
About this article
Cite this article
Jordan, D.R., Klein, R.R., Sakrewski, K.G. et al. Mapping and characterization of Rf 5 : a new gene conditioning pollen fertility restoration in A1 and A2 cytoplasm in sorghum (Sorghum bicolor (L.) Moench). Theor Appl Genet 123, 383–396 (2011). https://doi.org/10.1007/s00122-011-1591-y
- Fertility Restoration
- Restorer Gene
- Southern Corn Leaf Blight
- Fertility Restoration Gene