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Theoretical and Applied Genetics

, Volume 123, Issue 3, pp 383–396 | Cite as

Mapping and characterization of Rf 5 : a new gene conditioning pollen fertility restoration in A1 and A2 cytoplasm in sorghum (Sorghum bicolor (L.) Moench)

  • D. R. JordanEmail author
  • R. R. Klein
  • K. G. Sakrewski
  • R. G. Henzell
  • P. E. Klein
  • E. S. Mace
Original Paper

Abstract

With an aim to further characterize the cytoplasmic male sterility–fertility restoration system in sorghum, a major fertility restoration gene was mapped along with a second locus capable of partial restoration of pollen fertility. The major fertility restoration gene, Rf 5 , was located on sorghum chromosome SBI-05, and was capable of restoring pollen fertility in both A1 and A2 male sterile cytoplasms. Depending on the restorer parent, mapping populations exhibited fertility restoration phenotypes that ranged from nearly bimodal distribution due to the action of Rf 5 , to a more normalized distribution reflecting the action of Rf 5 and additional modifier/partial restoration genes. A second fertility restoration locus capable of partially restoring pollen fertility in A1 cytoplasm was localized to chromosome SBI-04. Unlike Rf 5 , this modifier/partial restorer gene acting alone resulted in less than 10% seed set in both A1 and A2 cytoplasms, and modified the extent of restoration conditioned by the major restorer Rf 5 in A1 cytoplasm. In examining the genomic regions spanning the Rf 5 locus, a cluster of pentatricopeptide gene family members with high homology to rice Rf 1 and sorghum Rf 2 were identified as potential candidates encoding Rf 5 .

Keywords

Sorghum Fertility Restoration Restorer Gene Southern Corn Leaf Blight Fertility Restoration Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors wish to thank Julie McCollum for her expert technical assistance. We thank the Australian Grains Research and Development Corporation (GRDC; http://www.grdc.com.au) for part of the financial support for this research, and the USDA-Agricultural Research Service for financial support to RRK.

Supplementary material

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Copyright information

© Her Majesty the Queen in Rights of Australia as represented by The State of Queensland 2011

Authors and Affiliations

  • D. R. Jordan
    • 1
    • 2
    Email author
  • R. R. Klein
    • 3
  • K. G. Sakrewski
    • 1
  • R. G. Henzell
    • 1
  • P. E. Klein
    • 4
  • E. S. Mace
    • 1
  1. 1.Agri-Science Queensland, Hermitage Research StationWarwickAustralia
  2. 2.Queensland Alliance for Agriculture and Food InnovationWarwickAustralia
  3. 3.USDA-ARS, Southern Plains Agricultural Research CenterCollege StationUSA
  4. 4.Department of Horticulture and Institute for Plant Genomics and BiotechnologyTexas A&M UniversityCollege StationUSA

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