Theoretical and Applied Genetics

, Volume 123, Issue 1, pp 55–68 | Cite as

Mapping Rph20: a gene conferring adult plant resistance to Puccinia hordei in barley

  • L. T. HickeyEmail author
  • W. Lawson
  • G. J. Platz
  • M. Dieters
  • V. N. Arief
  • S. Germán
  • S. Fletcher
  • R. F. Park
  • D. Singh
  • S. Pereyra
  • J. Franckowiak
Original Paper


A doubled haploid (DH) barley (Hordeum vulgare L.) population of 334 lines (ND24260 × Flagship) genotyped with DArT markers was used to map genes for adult plant resistance (APR) to leaf rust (Puccinia hordei Otth) under field conditions in Australia and Uruguay. The Australian barley cultivar Flagship carries an APR gene (qRphFlag) derived from the cultivar Vada. Association analysis and composite interval mapping identified two genes conferring APR in this DH population. qRphFlag was mapped to the short arm of chromosome 5H (5HS), accounting for 64–85% of the phenotypic variation across four field environments and 56% under controlled environmental conditions (CEC). A second quantitative trait locus (QTL) from ND24260 (qRphND) with smaller effect was mapped to chromosome 6HL. In the absence of qRphFlag, qRphND conferred only a low level of resistance. DH lines displaying the highest level of APR carried both genes. Sequence information for the critical DArT marker bPb-0837 (positioned at 21.2 cM on chromosome 5HS) was used to develop bPb-0837-PCR, a simple PCR-based marker for qRphFlag. The 245 bp fragment for bPb-0837-PCR was detected in a range of barley cultivars known to possess APR, which was consistent with previous tests of allelism, demonstrating that the qRphFlag resistant allele is common in leaf rust resistant cultivars derived from Vada and Emir. qRphFlag has been designated Rph20, the first gene conferring APR to P. hordei to be characterised in barley. The PCR marker will likely be effective in marker-assisted selection for Rph20.


Quantitative Trait Locus Doubled Haploid Leaf Rust Stripe Rust Doubled Haploid Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This research was supported by the Grains Research and Development Corporation of Australia through a Postgraduate Research Scholarship (GRS141) and Barley Breeding Australia, Northern Node (DAQ00110), and additional funding was provided for travel to field locations in Australia. The authors thank the University of Queensland for a Graduate School International Travel Award (GSITA) for assistance in collaborative research in Uruguay. We also wish to acknowledge Ms Julie McKavanagh and Mr Gary Bloustien (DEEDI) for their technical assistance in the laboratory and the field. Development of the DH lines was supported by the Co-operative Research Centre for an Internationally Competitive Pork Industry (Pork CRC Project 1A-101-0607).

Supplementary material

122_2011_1566_MOESM1_ESM.doc (94 kb)
Supplementary material 1 (DOC 93.5 kb)


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • L. T. Hickey
    • 1
    Email author
  • W. Lawson
    • 2
  • G. J. Platz
    • 2
  • M. Dieters
    • 1
  • V. N. Arief
    • 1
  • S. Germán
    • 3
  • S. Fletcher
    • 4
  • R. F. Park
    • 5
  • D. Singh
    • 5
  • S. Pereyra
    • 3
  • J. Franckowiak
    • 2
  1. 1.The University of Queensland, School of Agriculture and Food SciencesBrisbaneAustralia
  2. 2.Department of EmploymentEconomic Development and Innovation, Hermitage Research StationWarwickAustralia
  3. 3.Instituto Nacional de Investigación Agropecuaria, La EstanzuelaColoniaUruguay
  4. 4.Department of EmploymentEconomic Development and Innovation, Leslie Research Centre ToowoombaToowoombaAustralia
  5. 5.The University of Sydney, Plant Breeding Institute-CobbittyNarellanAustralia

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