Abstract
A relationship between pepper trichome and pepper mottle virus (PepMoV) resistance was examined. In an intraspecific F2 mapping population from the cross between Capsicum annuum CM334 (trichome-bearing and PepMoV resistant) and Chilsungcho (glabrous and PepMoV susceptible), major QTLs for both traits were identified by composite interval mapping in linkage group (LG) 24 corresponding a telomere region on pepper chromosome 10. Ptel1 of putative trichome enhancing locus was a common major QTL for trichome density on the main stem and calyx. Ptel1 apart from HpmsE031 at a 1.03 cM interval was specifically associated to the trichome density on the main stem, whereas Ptel2 near m104 marker on LG2 was specific for the calyx trichome. Epistatic analysis indicated that Ptel1 engaged in controlling the trichome density by mutual interactions with the organ-specific QTLs. For PepMoV resistance, two QTLs (Pep1 and Pep2) were identified on the LG 24. Pep1 was located with Ptel1 in the R-gene cluster (RGC) for potyvirus resistance including Pvr4 with broad spectrum resistance to potyviruses. Pep1 flanking TG420 marker seemed to be the major factors determining correlation with PepMoV resistance. These results indicate that the level of trichome density on pepper main stem can be used as a morphological marker for Pvr4 in pepper breeding.
Similar content being viewed by others
References
Ager FJ, Ynsa MD, Domnguez-Sol JR, Lopez-Mart MC, Gotor C, Romero LC (2003) Nuclear micro-probe analysis of Arabidopsis thaliana leaves. Nucl Instrum Methods Phys Res 210:401–406
Albar L, Lorieux M, Ahmadi N, Rimbault I, Pinel A, Sy AA, Fargette D, Ghesquiere A (1998) Genetic basis and mapping of the resistance to rice yellow mottle virus. I. QTLs identification and relationship between resistance and plant morphology. Theor Appl Genet 97:1145–1154
Caranta C, Thabuis A, Palloix A (1999) Development of a CAPS marker for the Pvr4 locus: a tool for pyramiding potyvirus resistance genes in pepper. Genome 42:1111–1116
Caranta C, Pflieger S, Lefebvre V, Daubeze AM, Thabuis A, Palloix A (2002) QTLs involved in the restriction of cucumber mosaic virus (CMV) long-distance movement in pepper. Theor Appl Genet 104:586–591
Dalin P, Bjorkman C (2003) Adult beetle grazing induces willow trichome defence against subsequent larval feeding. Oecologia 134:112–118
De Givry S, Bouchez M, Chabrier P, Milan D, Schiex T (2005) CARHTA GENE: multipopulation integrated genetic and radiation hybrid mapping. Bioinformatics 21:1703–1704
Doganlar S, Frary A, Daunay MC, Lester RN, Tanksley SD (2002) Conservation of gene function in the Solanaceae as revealed by comparative mapping of domestication traits in eggplant. Genetics 161:1713–1726
Dogimont C, Palloix A, Daubze AM, Marchoux G, Selassie KG, Pochard E (1996) Genetic analysis of broad spectrum resistance to potyviruses using doubled haploid lines of pepper (Capsicum annuum L.). Euphytica 88:231–239
Egea-Gilabert C, Bilotti G, Requena ME, Ezziyyani M, Vivo-Molina JM, Candela ME (2008) Pepper morphological traits related with resistance to Phytophthora capsici. Biol Plant 52:105–109
Elle E, van Dam NM, Hare JD (1999) Cost of glandular trichomes, a “resistance” character in Datura wrightii Regel (Solanaceae). Evolution 53:22–35
Espigares T, Peco B (1995) Mediterranean annual pasture dynamics: impact of autumn drought. J Ecol 83:135–142
Grube RC, Blauth JR, Arnedo Andres MS, Caranta C, Jahn MK (2000) Identification and comparative mapping of a dominant potyvirus resistance gene cluster in Capsicum. Theor Appl Genet 101:852–859
Han JH, Choi HS, Kim DH, Lee HR, Kim BD (2006) Biological, physical and cytological properties of pepper mottle virus-SNU1 and its RT-PCR detection. Plant Pathol J 22:155–160
Han JH, Shin JS, Kim YH, Kim BD (2007) Improvement of antigen blotting in a tissue blot immunobinding assay for the detection of two chili pepper viruses. J Microbe Biotechnol 17:1885–1889
Hanrahan LP, Mirkin I, Olson J, Anderson HA, Fiore BJ (1990) SMRFIT: a statistical analysis system (SAS) program for standardized mortality ratio analyses and Poisson regression model fits in community disease cluster investigations. Am J Epidemiol 132:116–122
Johnson HB (1975) Plant pubescence: an ecological perspective. Bot Rev 41:233–258
Kennedy GG (2003) Tomato, Pests, Parasitoids, and Predators: tritrophic interactions involving the genus Lycopersicon. Annu Rev Entomol 48:51–72
Kim HJ, Han JH, Kwon JK, Park M, Kim BD, Choi D (2010) Fine mapping of pepper trichome locus 1 controlling trichome formation in Capsicum annuum L. CM334. Theor Appl Genet 120:1099–1106
Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175
Lee HR, Bae IH, Park SW, Kim HJ, Min WK, Han JH, Kim KT, Kim BD (2009) Construction of an integrated pepper map using RFLP, SSR, CAPS, AFLP, WRKY, rRAMP, and BAC end sequences. Mol Cells 27:21–37
Levin DA (1973) The role of trichomes in plant defense. Quart Rev Biol 48:3–15
Marks MD (1997) Molecular genetic analysis of trichome development in Arabidopsis. Annu Rev Plant Biol 48:137–163
Mei M, Syed NH, Gao W, Thaxton PM, Smith CW, Stelly DM, Chen ZJ (2004) Genetic mapping and QTL analysis of fiber-related traits in cotton (Gossypium). Theor Appl Genet 108:280–291
Min WK, Han JH, Kang WH, Lee HR, Kim BD (2008) Reverse random amplified microsatellite polymorphism reveals enhanced polymorphisms in the 3 end of simple sequence repeats in the pepper genome. Mol Cells 26:250–257
Murphy JF, Blauth JR, Livingstone KD, Lackney VK, Jahn MK (1998) Genetic mapping of the pvr1 locus in Capsicum spp. and evidence that distinct potyvirus resistance loci control responses that differ at the whole plant and cellular levels. Mol Plant Microbe Interact 11:943–951
Skaltsa H, Verykokidou E, Harvala C, Karabourniotis G, Manetasi Y (1994) UV-B protective potential and flavonoid content of leaf hairs of Quercus ilex. Phytochemistry 37:987–990
Tanksley SD, Ganal MW, Prince JP, De-Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB (1992) High density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160
Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78
Wang S, Basten CJ, Zeng Z (2004) Window QTL Cartographer. V. 2.0 Program in Statistical Genetics. North Carolina State University, North Carolina
Wilkins TA, Rajasekaran K, Anderson DM (2000) Cotton biotechnology. Crit Rev Plant Sci 19:511–550
Zeng ZB (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468
Acknowledgments
This work was supported by a grant (project No. 609002-5) from the Screening Center for Disease Resistant Vegetable Crops of Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries and the Crop Functional Genomics Center, 21st Frontier Research Program of MEST of the Korean government.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by I. Paran.
Rights and permissions
About this article
Cite this article
Kim, H.J., Han, JH., Kim, S. et al. Trichome density of main stem is tightly linked to PepMoV resistance in chili pepper (Capsicum annuum L.). Theor Appl Genet 122, 1051–1058 (2011). https://doi.org/10.1007/s00122-010-1510-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00122-010-1510-7