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A new chromosome nomenclature system for oat (Avena sativa L. and A. byzantina C. Koch) based on FISH analysis of monosomic lines

Abstract

Fluorescent in situ hybridization (FISH) with multiple probes was used to analyze mitotic and meiotic chromosome spreads of Avena sativa cv ‘Sun II’ monosomic lines, and of A. byzantina cv ‘Kanota’ monosomic lines from spontaneous haploids. The probes used were A. strigosa pAs120a (a repetitive sequence abundant in A-genome chromatin), A. murphyi pAm1 (a repetitive sequence abundant in C-genome chromatin), A. strigosa pITS (internal transcribed spacer of rDNA) and the wheat rDNA probes pTa71 (nucleolus organizer region or NOR) and pTa794 (5S). Simultaneous and sequential FISH employing pairs of these probes allowed the identification and genome assignation of all chromosomes. FISH mapping using mitotic and meiotic metaphases facilitated the genomic and chromosomal identification of the monosome in each line. Of the 17 ‘Sun II’ lines analyzed, 13 distinct monosomic lines were found, corresponding to four monosomes of the A-genome, five of the C-genome and four of the D-genome. In addition, 12 distinct monosomic lines were detected among the 20 ‘Kanota’ lines examined, corresponding to six monosomes of the A-genome, three of the C-genome and three of the D-genome. The results show that 19 chromosomes out of 21 of the complement are represented by monosomes between the two genetic backgrounds. The identity of the remaining chromosomes can be deduced either from one intergenomic translocation detected on both ‘Sun II’ and ‘Kanota’ lines, or from the single reciprocal, intergenomic translocation detected among the ‘Sun II’ lines. These results permit a new system to be proposed for numbering the 21 chromosome pairs of the hexaploid oat complement. Accordingly, the A-genome contains chromosomes 8A, 11A, 13A, 15A, 16A, 17A and 19A; the C-genome contains chromosomes 1C, 2C, 3C, 4C, 5C, 6C and 7C; and the D-genome consists of chromosomes 9D, 10D, 12D, 14D, 18D, 20D and 21D. Moreover, the FISH patterns of 16 chromosomes in ‘Sun II’ and 15 in ‘Kanota’ suggest that these chromosomes could be involved in intergenomic translocations. By comparing the identities of individually translocated chromosomes in the two hexaploid species with those of other hexaploids, we detected different types of intergenomic translocations.

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References

  1. Chen Q, Armstrong K (1994) Genomic in situ hybridization in Avena sativa. Genome 37:607–612

    CAS  Article  PubMed  Google Scholar 

  2. Coffman FA (1946) Origin of cultivated oats. J Am Soc Agron 38:983–1002

    Google Scholar 

  3. Davis DW, Rines HW (1991) Characterization of aneuploid and euploid progeny derived from oat haploids. In: Agronomy abstracts. ASA, Madison, p 91

  4. Fominaya A, Hueros G, Loarce Y, Ferrer E (1995) Chromosomal distribution of a repeated DNA from C-genome heterochromatin and the identification of a new ribosomal DNA locus in the Avena genus. Genome 38:548–557

    CAS  PubMed  Google Scholar 

  5. Fox SL, Jellen EN, Kianian SF, Rines HW, Phillips RL (2001) Assignment of RFLP linkage groups to chromosomes using monosomic F1 analysis in hexaploid oat. Theor Appl Genet 102:320–326

    CAS  Article  Google Scholar 

  6. Gerlach WL, Bedbrook JR (1979) Cloning and characterization of RNA genes from wheat and barley. Nucleic Acids Res 7:1869–1885

    CAS  Article  PubMed  Google Scholar 

  7. Gerlach WL, Dyer TA (1980) Sequence organization of the repeated units in the nucleus of the wheat which contains 5S-rRNA genes. Nucleic Acids Res 8:4851–4865

    CAS  Article  PubMed  Google Scholar 

  8. Gill BS (1991) Nucleocytoplasmic interaction (NCI) hypothesis of genome evolution and speciation in polyploid plants. In: Sasakuma T, Kinoshita T (eds) Proceedings of the Kihara memorial international symposium on cytoplasmic engineering in wheat. Yokohama, pp 48–53

  9. Hacker JB, Riley R (1965) Morphological and cytological effects of chromosome deficiency in Avena sativa. Can J Genet Cytol 7:304–315

    Google Scholar 

  10. Hayasaki M, Morikawa T, Tarumoto I (2000) Intergenomic translocations of polyploidy oats (genus Avena) revealed by genomic in situ hybridization. Genes Genet Syst 74:167–171

    Article  Google Scholar 

  11. Irigoyen ML, Linares C, Ferrer E, Fominaya A (2002) Fluorescent in situ hybridization mapping of Avena sativa L. cv. ‘Sun II’ and its monosomic lines using cloned repetitive DNA sequences. Genome 45:1230–1237

    CAS  Article  PubMed  Google Scholar 

  12. Jellen EN, Beard J (2000) Geographical distribution of a chromosome 7C and 17 intergenomic translocation in cultivated oat. Crop Sci 400:256–263

    Article  Google Scholar 

  13. Jellen EN, Phillips RL, Rines HW (1993a) C-banded karyotypes and polymorphisms in hexaploid oat accessions (Avena spp.) using Wright′s stain. Genome 36:1129–1137

    CAS  Article  PubMed  Google Scholar 

  14. Jellen EN, Rooney WL, Phillips RL, Rines HW (1993b) Characterization of the hexaploid oat Avena byzantina cv. ‘Kanota’ monosomic series using C-banding and RFLPs. Genome 36:962–970

    CAS  Article  PubMed  Google Scholar 

  15. Jellen EN, Gill BS, Cox TS (1994) Genomic in situ hybridization differentiates between A/D- and C-genome chromatin and detects intergenomic translocations in polyploidy oat species (genus Avena). Genome 37:613–618

    CAS  Article  PubMed  Google Scholar 

  16. Jellen EN, Rines HW, Fox SL, Davis DW, Phillips RL, Gill BS (1997) Characterization of ‘Sun II’ oat monosomics through C-banding and identification of eight new ‘Sun II’ monosomics. Theor Appl Genet 95:1190–1195

    Article  Google Scholar 

  17. Khush GS (1973) Cytogenetics of aneuploids. Academic Press, New York, pp 153–189

    Google Scholar 

  18. Leggett JM, Markhand SM (1995) The genomic identification of some monosomics of Avena sativa L. cv. ‘Sun II’ using genomic in situ hybridization. Genome 38:747–751

    CAS  Article  PubMed  Google Scholar 

  19. Linares C, Vega C, Ferrer E, Fominaya A (1992) Identification of C-banded chromosomes in meiosis and the analysis of nucleolar activity in Avena byzantina C. Koch cv ‘Kanota’. Theor Appl Genet 83:650–654

    Article  Google Scholar 

  20. Linares C, Gonzalez J, Ferrer E, Fominaya A (1996) The use of double fluorescence in situ hybridization to physically map the positions of 5S rDNA genes in relation to the chromosomal location of 18S-5.8S-26S rDNA and a C genome specific DNA sequence in the genus Avena. Genome 39:535–542

    CAS  Article  PubMed  Google Scholar 

  21. Linares C, Ferrer E, Fominaya A (1998) Discrimination of the closely related A and D genomes of the hexaploid oat Avena sativa L. Proc Natl Acad Sci USA 95:12450–12455

    CAS  Article  PubMed  Google Scholar 

  22. Linares C, Irigoyen ML, Fominaya A (2000) Identification of C-genome chromosomes involved in intergenomic translocations in Avena sativa L., using cloned repetitive DNA sequences. Theor Appl Genet 100:353–360

    CAS  Article  Google Scholar 

  23. Linares C, Loarce Y, Serna A, Fominaya A (2001) Isolation and characterization of two novel retrotransposon of the Ty1-copia group in oat genomes. Chromosome 110:115–123

    CAS  Article  Google Scholar 

  24. Mendu N, Rines HW, Silflow CD (1993) Mapping of beta-tubulin genomic sequences in hexaploid oat (Avena sativa L.). Theor Appl Genet 86:135–140

    CAS  Article  Google Scholar 

  25. Morikawa T (1985) Identification of the 21 monosomic lines in Avena byzantina C. Koch cv ‘Kanota′. Theor Appl Genet 70:271–278

    Article  Google Scholar 

  26. Rajhathy T, Thomas H (1974) Cytogenetics of oats (Avena L.). Misc Publ Genet Soc Can 2:1–90

    Google Scholar 

  27. Rines HW, Dahleen LS (1990) Haploid oat plants produced by application of maize pollen to emasculated oat florets. Crop Sci 30:1073–1078

    Article  Google Scholar 

  28. Rooney WL, Jellen EN, Phillips RL, Rines HW, Kianian SF (1994) Identification of homeologous chromosomes in hexaploid oat (Avena byzantina cv ‘Kanota’) using monosomics and RFLP analysis. Theor Appl Genet 89:329–335

    CAS  Article  Google Scholar 

  29. Solano R, Hueros G, Fominaya A, Ferrer E (1992) Organization of repeated sequences in species of the genus Avena. Theor Appl Genet 83:602–607

    Article  Google Scholar 

  30. Thomas H (1992) Cytogenetics of Avena. In: Marshall HG, Sorrells ME (eds) Oat science and technology. Madison; Agron Monogr 33 ASA, CSSA, pp 473–507

  31. Ueno M, Morikawa T (2007) Production of synthetic polyploid oats and detection of C genome rearrangements by GISH and FISH. Breed Sci 57:339–343

    CAS  Article  Google Scholar 

  32. Yang Q, Hanson L, Bennett MD, Leitch IJ (1999) Genome structure and evolution in the allohexaploid weed Avena fatua L. (Poaceae). Genome 42:512–518

    CAS  Article  PubMed  Google Scholar 

  33. Zhou X, Jellen EN, Murphy JP (1999) Progenitor germplasm of domesticated hexaploid oat. Crop Sci 39:1208–1214

    Article  Google Scholar 

Download references

Acknowledgments

The authors thank Dr. H. W. Rines of the Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, USA, for providing the ‘Sun II’ monosomic lines derived from oat/maize hybrids and Dr. T. Morikawa of the University of Osaka Prefecture (Osaka), for providing the monosomic lines of A. byzantina C. Koch cv ‘Kanota’. The authors also thank the Spanish Ministerio de Educación y Ciencia (AGL2006-04165) and the Comunidad de Madrid-Universidad de Alcalá (CCG06-UAH/GEV-0380) for their funding of this study.

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Correspondence to A. Fominaya.

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Communicated by B. Friebe.

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Sanz, M.J., Jellen, E.N., Loarce, Y. et al. A new chromosome nomenclature system for oat (Avena sativa L. and A. byzantina C. Koch) based on FISH analysis of monosomic lines. Theor Appl Genet 121, 1541–1552 (2010). https://doi.org/10.1007/s00122-010-1409-3

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Keywords

  • Chromosome Pair
  • Aneuploid Chromosome
  • Monosomic Line
  • Intergenomic Translocation
  • Monosomic Chromosome