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A survey of EMS-induced biennial Beta vulgaris mutants reveals a novel bolting locus which is unlinked to the bolting gene B

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Abstract

Beta vulgaris is a facultative perennial species which exhibits large intraspecific variation in vernalization requirement and includes cultivated biennial forms such as the sugar beet. Vernalization requirement is under the genetic control of the bolting locus B on chromosome II. Previously, ethyl methanesulfonate (EMS) mutagenesis of an annual accession had yielded several mutants which require vernalization to bolt and behave as biennials. Here, five F2 populations derived from crosses between biennial mutants and annual beets were tested for co-segregation of bolting phenotypes with genotypic markers located at the B locus. One mutant appears to be mutated at the B locus, suggesting that an EMS-induced mutation of B can be sufficient to abolish annual bolting. Co-segregation analysis in four populations indicates that the genetic control of bolting also involves previously unknown major loci not linked to B, one of which also affects bolting time and was genetically mapped to chromosome IX.

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Acknowledgments

The project was funded by the Deutsche Forschungsgemeinschaft under grant no. DFG JU 205/14-1. S. F. Abou-Elwafa is supported by a scholarship from the Ministry of Higher Education, Egypt. We thank Uwe Hohmann for propagation of mutants, Gretel Schulze-Buxloh for marker sequence information, Monika Bruisch and Erwin Danklefsen for technical assistance in the field and greenhouse, and Martina Bach and Monika Dietrich for technical assistance in the laboratory.

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  1. Wenying Zhang

    Present address: Agricultural College, Yangtze University, Jingzhou, 434025, Hubei Province, People’s Republic of China

Authors and Affiliations

  1. Plant Breeding Institute, Christian-Albrechts-University of Kiel, Olshausenstr. 40, 24098, Kiel, Germany

    Bianca Büttner, Salah F. Abou-Elwafa, Wenying Zhang, Christian Jung & Andreas E. Müller

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  1. Bianca Büttner
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  2. Salah F. Abou-Elwafa
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  3. Wenying Zhang
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Correspondence to Andreas E. Müller.

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Communicated by T. Luebberstedt.

B. Büttner and S. F. Abou-Elwafa contributed equally to this work.

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Büttner, B., Abou-Elwafa, S.F., Zhang, W. et al. A survey of EMS-induced biennial Beta vulgaris mutants reveals a novel bolting locus which is unlinked to the bolting gene B . Theor Appl Genet 121, 1117–1131 (2010). https://doi.org/10.1007/s00122-010-1376-8

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