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Identification of a second major resistance gene to Rice yellow mottle virus, RYMV2, in the African cultivated rice species, O. glaberrima

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Abstract

Rice yellow mottle virus (RYMV) is the most damaging rice-infecting virus in Africa. However, few sources of high resistance and only a single major resistance gene, RYMV1, are known to date. We screened a large representative collection of African cultivated rice (Oryza glaberrima) for RYMV resistance. Whereas high resistance is known to be very rare in Asian cultivated rice (Oryza sativa), we identified 29 (8%) highly resistant accessions in O. glaberrima. The MIF4G domain of RYMV1 was sequenced in these accessions. Some accessions possessed the rymv1-3 or rymv1-4 recessive resistance alleles previously described in O. glaberrima Tog5681 and Tog5672, respectively, and a new allele, rymv1-5, was identified, thereby increasing the number of resistance alleles in O. glaberrima to three. In contrast, only a single allele has been reported in O. sativa. Markers specific to the different alleles of the RYMV1 gene were developed for marker-assisted selection of resistant genotypes for disease management. In addition, the presence of the dominant susceptibility allele (Rymv1-1) in 15 resistant accessions suggests that their resistance is under different genetic control. An allelism test involving one of those accessions revealed a second major resistance gene, i.e., RYMV2. The diversity of resistance genes against RYMV in O. glaberrima species is discussed in relation to the diversification of the virus in Africa.

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Acknowledgments

This work was supported by a PhD grant to D. Thiémélé through the French-speaking University Network (AUF), and by Generation Challenge Program (GCP) (Project No. G3007.01: iBridges). A GCP Fellowships & Travel Grant (Project No. G4007.14) supported D. Thiémélé’s stay at the Africa Rice Center (WARDA) for RYMV resistance evaluation. We thank WARDA and the International Rice Research Institute (IRRI) for providing most of the O. glaberrima accessions. We gratefully acknowledge Agnès Pinel-Galzi for help in VPg sequencing, and Denis Fargette and Thomas Roscoe for critical reviews of the manuscript.

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Author notes

  1. Arnaud Boisnard

    Present address: Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK

Authors and Affiliations

  1. Laboratoire Génome et Développement des Plantes, Unité Mixte de Recherche 5096, Université de Perpignan via Domitia, CNRS, IRD, BP 64501, 34394, Montpellier Cedex 5, France

    Deless Thiémélé, Arnaud Boisnard, Sophie Chéron, Alain Ghesquière & Laurence Albar

  2. Laboratoire de Physiologie Végétale, Université de Cocody, 22 B.P. 582, Abidjan, Ivory Coast

    Deless Thiémélé & Séverin Aké

  3. Africa Rice Center, WARDA, 01 BP 2031, Cotonou, Benin

    Marie-Noëlle Ndjiondjop & Yacouba Séré

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  1. Deless Thiémélé
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Correspondence to Laurence Albar.

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Communicated by T. Sasaki.

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Thiémélé, D., Boisnard, A., Ndjiondjop, MN. et al. Identification of a second major resistance gene to Rice yellow mottle virus, RYMV2, in the African cultivated rice species, O. glaberrima . Theor Appl Genet 121, 169–179 (2010). https://doi.org/10.1007/s00122-010-1300-2

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