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Identification of QTLs that control clubroot resistance in Brassica oleracea and comparative analysis of clubroot resistance genes between B. rapa and B. oleracea

Theoretical and Applied Genetics volume 120, pages 1335–1346 (2010)Cite this article

Abstract

To perform comparative studies of CR (clubroot resistance) loci in Brassica oleracea and Brassica rapa and to develop marker-assisted selection in B. oleracea, we constructed a B. oleracea map, including specific markers linked to CR genes of B. rapa. We also analyzed CR-QTLs using the mean phenotypes of F3 progenies from the cross of a resistant double-haploid line (Anju) with a susceptible double-haploid line (GC). In the nine linkage groups obtained (O1-O9), the major QTL, pb-Bo(Anju)1, was derived from Anju with a maximum LOD score (13.7) in O2. The QTL (LOD 5.1) located in O5, pb-Bo(GC)1, was derived from the susceptible GC. Other QTLs with smaller effects were found in O2, O3, and O7. Based on common markers, it was possible to compare our finding CR-QTLs with the B. oleracea CR loci reported by previous authors; pb-Bo(GC)1 may be identical to the CR-QTL reported previously or a different member contained in the same CR gene cluster. In total, the markers linked to seven B. rapa CR genes were mapped on the B. oleracea map. Based on the mapping position and markers of the CR genes, informative comparative studies of CR loci between B. oleracea and B. rapa were performed. Our map discloses specific primer sequences linked to CR genes and includes public SSR markers that will promote pyramiding CR genes in intra- and inter-specific crosses in Brassica crops. Five genes involved in glucosinolates biosynthesis were also mapped, and GSL-BoELONG and GSL-BoPro were found to be linked to the pb-Bo(Anju)1 and Bo(GC)1 loci, respectively. The linkage drag associated with the CR-QTLs is briefly discussed.

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Acknowledgments

The authors sincerely thank Dr. K. Hatakeyama from the National Institute of Vegetable and Tea Science, Japan, for kindly providing DH lines.

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Affiliations

  1. Graduate School of Science and Technology, Niigata University, Ikarashi, Niigata, 950-2181, Japan

    T. Nagaoka, T. Ishikawa, H. Hori & K. Okazaki

  2. Faculty of Agriculture, Sylhet Agricultural University (SAU), Sylhet, 3100, Bangladesh

    M. A. U. Doullah

  3. National Institute of Vegetable and Tea Science (NIVTS), Ano, Tsu, Mie, 514-2392, Japan

    S. Matsumoto

  4. National Institute of Agrobiological Sciences (NIAS), Tsukuba, Ibaraki, 305–8602, Japan

    S. Kawasaki

  5. Faculty of Agriculture, Niigata University, Ikarashi, Niigata, 950-2181, Japan

    K. Okazaki

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  1. T. Nagaoka
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  2. M. A. U. Doullah
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  3. S. Matsumoto
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  4. S. Kawasaki
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  5. T. Ishikawa
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  6. H. Hori
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  7. K. Okazaki
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Correspondence to K. Okazaki.

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Communicated by M. Havey.

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Nagaoka, T., Doullah, M.A.U., Matsumoto, S. et al. Identification of QTLs that control clubroot resistance in Brassica oleracea and comparative analysis of clubroot resistance genes between B. rapa and B. oleracea . Theor Appl Genet 120, 1335–1346 (2010). https://doi.org/10.1007/s00122-010-1259-z

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  • DOI: https://doi.org/10.1007/s00122-010-1259-z

Keywords

  • Cleave Amplify Polymorphic Sequence
  • Disease Severity Index
  • Restriction Fragment Length Polymorphism Marker
  • Clubroot Resistance
  • Bacterial Artificial Chromosome Sequence