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Development of wheat lines carrying stem rust resistance gene Sr39 with reduced Aegilops speltoides chromatin and simple PCR markers for marker-assisted selection

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Abstract

The use of major resistance genes is a cost-effective strategy for preventing stem rust epidemics in wheat crops. The stem rust resistance gene Sr39 provides resistance to all currently known pathotypes of Puccinia graminis f. sp. tritici (Pgt) including Ug99 (TTKSK) and was introgressed together with leaf rust resistance gene Lr35 conferring adult plant resistance to P. triticina (Pt), into wheat from Aegilops speltoides. It has not been used extensively in wheat breeding because of the presumed but as yet undocumented negative agronomic effects associated with Ae. speltoides chromatin. This investigation reports the production of a set of recombinants with shortened Ae. speltoides segments through induction of homoeologous recombination between the wheat and the Ae. speltoides chromosome. Simple PCR-based DNA markers were developed for resistant and susceptible genotypes (Sr39#22r and Sr39#50s) and validated across a set of recombinant lines and wheat cultivars. These markers will facilitate the pyramiding of ameliorated sources of Sr39 with other stem rust resistance genes that are effective against the Pgt pathotype TTKSK and its variants.

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Acknowledgments

The authors are thankful to Xiaodi Xia for providing excellent technical assistance. The authors are thankful to the Grains Research and Development Corporation, Australia for financial support through the Australian Cereal Rust Control Program.

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Authors and Affiliations

  1. CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia

    Rohit Mago & J. G. Ellis

  2. The University of Sydney, Plant Breeding Institute, Cobbitty, Private Mail Bag 11, Camden, NSW, 2570, Australia

    P. Zhang, H. S. Bariana & U. K. Bansal

  3. School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Private Mail Bag 1, Glen Osmond, SA, 5064, Australia

    D. C. Verlin & I. S. Dundas

Authors
  1. Rohit Mago
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  2. P. Zhang
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  3. H. S. Bariana
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  4. D. C. Verlin
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  5. U. K. Bansal
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  6. J. G. Ellis
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  7. I. S. Dundas
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Corresponding author

Correspondence to Rohit Mago.

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Communicated by B. Keller.

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Mago, R., Zhang, P., Bariana, H.S. et al. Development of wheat lines carrying stem rust resistance gene Sr39 with reduced Aegilops speltoides chromatin and simple PCR markers for marker-assisted selection. Theor Appl Genet 119, 1441–1450 (2009). https://doi.org/10.1007/s00122-009-1146-7

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  • DOI: https://doi.org/10.1007/s00122-009-1146-7

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