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Meta-QTL analysis of the genetic control of ear emergence in elite European winter wheat germplasm

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Variation in ear emergence time is critical for the adaptation of wheat (Triticum aestivum L.) to specific environments. The aim of this study was to identify genes controlling ear emergence time in elite European winter wheat germplasm. Four doubled haploid populations derived from the crosses: Avalon × Cadenza, Savannah × Rialto, Spark × Rialto, and Charger × Badger were selected which represent diversity in European winter wheat breeding programmes. Ear emergence time was recorded as the time from 1st May to heading in replicated field trials in the UK, France and Germany. Genetic maps based on simple sequence repeat (SSR) and Diversity Arrays Technology (DArT) markers were constructed for each population. One hundred and twenty-seven significant QTL were identified in the four populations. These effects were condensed into 19 meta-QTL projected onto a consensus SSR map of wheat. These effects are located on chromosomes 1B (2 meta-QTL), 1D, 2A (2 meta-QTL), 3A, 3B (2 meta-QTL), 4B, 4D, 5A (2 meta-QTL), 5B, 6A, 6B 7A (2 meta-QTL), 7B and 7D. The identification of environmentally robust earliness per se effects will facilitate the fine tuning of ear emergence in predictive wheat breeding programmes.

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  • Arcade A, Labourdette A, Falque M, Mangin B, Chardon F, Charcosset A, Joets J (2004) BioMercator: integrating genetic maps and QTL towards discovery of candidate genes. Bioinformatics 20:2324–2326

    Article  PubMed  CAS  Google Scholar 

  • Bassam BJ, Caetanoanolles G, Gresshoff PM (1991) Fast and Sensitive Silver Staining of DNA in Polyacrylamide Gels. Anal Biochem 196:80–83

    Article  PubMed  CAS  Google Scholar 

  • Bertin I, Zhu JH, Gale MD (2005) SSCP-SNP in pearl millet—a new marker system for comparative genetics. Theor Appl Genet 110L:1467–1472

    Article  CAS  Google Scholar 

  • Bonnin I, Rousset M, Madur D, Sourdille P, Dupuits L, Brunel D, Goldringer I (2008) FT genome A and D polymorphisms are associated with the variation of earliness components in hexaploid wheat. Theor Appl Genet 116:383–394

    Article  PubMed  CAS  Google Scholar 

  • Borner A, Buck-Sorlin GH, Hayes PM, Malyshev S, Korzun V (2002) Molecular mapping of major genes and quantitative trait loci determining flowering time in response to photoperiod in barley. Plant Breeding 121:129–132

    Article  CAS  Google Scholar 

  • Darvasi A, Soller M (1997) A simple method to calculate resolving power and confidence interval of QTL map location. Behav Genet 27:125–132

    Article  PubMed  CAS  Google Scholar 

  • Faure S, Higgins J, Turner A, Laurie DA (2007) The FLOWERING LOCUS T-like gene family in barley (Hordeum vulgare). Genetics 176:599–609

    Article  PubMed  CAS  Google Scholar 

  • Flintham JE, Borner A, Worland AJ, Gale MD (1997) Optimizing wheat grain yield: effects of Rht (gibberellin-insensitive) dwarfing genes. J Agric Sci 128:11–25

    Article  Google Scholar 

  • Hanocq E, Laperche A, Jaminon O, Laine AL, Le Gouis J (2007) Most significant genome regions involved in the control of earliness traits in bread wheat, as revealed by QTL meta-analysis. Theor Appl Genet 114:569–584

    Article  PubMed  CAS  Google Scholar 

  • Hoogendoorn J (1985) A reciprocal F1 monosomic of the genetic control of ear emergencenumber of eaves and spikelets in wheat (Triticum aestivum L). Euphytica 34:545–558

    Article  Google Scholar 

  • IslamFaridi MN, Worland AJ, Law CN (1996) Inhibition of ear-emergence time and sensitivity to day-length determined by the group 6 chromosomes of wheat. Heredity 77:572–580

    Article  Google Scholar 

  • Kuchel H, Hollamby G, Langridge P, Williams K, Jefferies SP (2006) Identification of genetic loci associated with ear-emergence in bread wheat. Theor Appl Genet 113:1103–1112

    Article  PubMed  CAS  Google Scholar 

  • Laurie DA, Pratchett N, Bezant JH, Snape JW (1995) Rflp mapping of 5 major genes and 8 quantitative trait loci controlling flowering time in a winter × spring barley (Hordeum-Vulgare L) Cross. Genome 38:575–585

    PubMed  CAS  Google Scholar 

  • Law CN (1966) The location of genetic factors affecting a quantitative character in wheat. Genetics 53:487–498

    Google Scholar 

  • Law CN, Worland AJ (1997) Genetic analysis of some flowering time and adaptive traits in wheat. New Phytol 137:19–28

    Article  Google Scholar 

  • Law CN, Worland AJ, Giorgi B (1976) Genetic-control of ear-emergence time by chromosomes-5a and chromosomes-5D of wheat. Heredity 36:49–58

    Article  Google Scholar 

  • Law CN, Suarez E, Miller TE, Worland AJ (1998) The influence of the group 1 chromosomes of wheat on ear-emergence times and their involvement with vernalization and day length. Heredity 80:83–91

    Article  Google Scholar 

  • Miralles DJ, Slafer GA (2007) Sink limitations to yield in wheat: how could it be reduced? J Agric Sci 145:139–149

    Article  Google Scholar 

  • Miura H, Worland AJ (1994) Genetic-control of vernalization, day-length response, and earliness per se by homoeologous group-3 chromosomes in wheat. Plant Breeding 113:160–169

    Article  Google Scholar 

  • Miura H, Nakagawa M, Worland AJ (1999) Control of ear emergence time by chromosome 3A of wheat. Plant Breeding 118:85–87

    Article  Google Scholar 

  • Pankova K, Milec Z, Leverington-Waite M, Chebotar S, Snape JW (2008) Characterization of inter-varietal chromosome substitution lines of wheat using molecular markers. Czech J Genet Plant Breed 44:22–29

    CAS  Google Scholar 

  • Scarth R, Law CN (1984) The control of the day-length response in wheat by the group 2 chromosomes. Z Pflanzenzuchtg 92:140–150

    Google Scholar 

  • Shah MM, Baenziger PS, Yen Y, Gill KS, Moreno-Sevilla B, Haliloglu K (1999) Genetic analyses of agronomic traits controlled by wheat chromosome 3A. Crop Sci 39:1016–1021

    Article  Google Scholar 

  • Snape JW, Butterworth K, Whitechurch E, Worland AJ (2001) Waiting for fine times: genetics of flowering time in wheat. Euphytica 119:185–190

    Article  CAS  Google Scholar 

  • Snape JW, Foulkes MJ, Simmonds J, Leverington M, Fish LJ, Wang Y, Ciavarrella M (2007) Dissecting gene x environmental effects on wheat yields via QTL and physiological analysis. Euphytica 154:401–408

    Article  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L). Theor Appl Genet 109:1105–1114

    Article  PubMed  CAS  Google Scholar 

  • Valarik M, Linkiewicz A, Dubcovsky J (2006) A microcolinearity study at the earliness per se gene Eps-A(m)1 region reveals an ancient duplication that preceded the wheat-rice divergence. Theor Appl Genet 112:945–957

    Article  PubMed  CAS  Google Scholar 

  • Wang SBC, Zeng ZB (2007) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh

  • Wenzl P, Carling J, Kudrna D, Jaccoud D, Huttner E, Kleinhofs A, Kilian A (2004) Diversity Arrays Technology (DArT) for whole-genome profiling of barley. Proc Natl Acad Sci USA 101:9915–9920

    Article  PubMed  CAS  Google Scholar 

  • Worland AJ (1996) The influence of flowering time genes on environmental adaptability in European wheats. Euphytica 89:49–57

    Article  Google Scholar 

  • Yan L, Helguera M, Kato K, Fukuyama S, Sherman J, Dubcovsky J (2004) Allelic variation at the VRN-1 promoter region in polyploid wheat. Theor Appl Genet 109:1677–1686

    Article  PubMed  CAS  Google Scholar 

  • Yan L, Fu D, Li C, Blechl A, Tranquilli G, Bonafede M, Sanchez A, Valarik M, Dubcovsky J (2006) The wheat and barley vernalization gene VRN3 is an orthologue of FT. Proc Natl Acad Sci USA 103:19581–19586

    Article  PubMed  CAS  Google Scholar 

  • Yano M, Kojima S, Takahashi Y, Lin HX, Sasaki T (2001) Genetic control of flowering time in rice, a short-day plant. Plant Physiol 127:1425–1429

    Article  PubMed  CAS  Google Scholar 

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We thank Dr Simon Berry of Limagrain UK for the supply of SSR data for the Savannah x Rialto population. The work was supported by funding from the UK Biotechnology and Biological Sciences Research Council (BBSRC) and the UK Department of the Environment Food and Rural Affairs (DEFRA), the latter through a grant for the Wheat Genetic Improvement Network.

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Correspondence to Simon Griffiths.

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Communicated by J. Dubcovsky.

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Griffiths, S., Simmonds, J., Leverington, M. et al. Meta-QTL analysis of the genetic control of ear emergence in elite European winter wheat germplasm. Theor Appl Genet 119, 383–395 (2009).

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