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Genetics and mapping of adult plant rust resistance in soybean PI 587886 and PI 587880A

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Abstract

Two soybean accessions, PI 587886 and PI 587880A, previously identified as having resistance to Phakospora pachyrhizi Syd. (soybean rust, SBR) were used to create two populations (POP-1 and POP-2) segregating for SBR resistance. F2-derived F3 (F2:3) families from each population were grown in a naturally SBR-infected field in Paraguay to determine inheritance and map resistance genes. Over 6,000 plants from 178 families in POP-1 and over 5,000 plants from 160 families in POP-2 were evaluated at R5 for lesion type: immune reaction (IR), reddish-brown (RB), or tan (TAN) colored lesions. Based on the lesion type present, each F2:3 family was rated as resistant, segregating or susceptible and this classification was used to infer the F2-phenotype and genotype. For both populations, the F2 segregation ratios fit a 1:2:1 (resistant:segregating:susceptible) ratio expected for a single gene (P > 0.05). The RB lesions occurred almost exclusively in the heterozygous class, indicating incomplete dominance under the conditions of this study. Molecular markers flanking the locations of the known resistance genes were used to map the resistance gene in both populations to the Rpp1 locus. However, evaluation of PI 587886 and PI 587880A against eight P. pachyrhizi isolates indicated that the resistance allele in these two accessions was different from Rpp1. This test also demonstrated that these accessions were resistant to at least one P. pachyrhizi isolate collected in the southern US. This is the first report of using an adult plant field-screen with natural rust pressure to map SBR resistance.

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Acknowledgments

The authors appreciate the dedicated work of the USDA-ARS personnel at Stoneville, MS, including Ms. Angelie Davis, Mr. Hans Hinrichsen, Mr. Philip Handly, Mr. Ronnie Griffin, Mr. Jeffrey Britt and Ms. Loria Courtney. In Paraguay we were ably assisted in the field and laboratory by Ms. Noelia Bogado, Mr. Ismael Morel, Mr. Francisco Saucedo, Mr. Claudio Vigo, Ms. Diana Bado, Ms. Sergia Portillo, and Ms. Nidia Benitez. The Paraguay based portion of this work was critically facilitated by Mr. Luis Enrique Robledo of DIA and Mr. Luis E. Cubilla of CAPECO. Seed production in Puerto Rico by Ricardo Goenaga and Carlos Torres of the USDA-ARS Tropical Agriculture Research Station was critical for the timely completion of this project. We also appreciate the efforts of Ms. Christine Stone for assistance with the inoculations at the FDWSRU. This research could not have been accomplished without these combined efforts.

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Correspondence to Jeffery D. Ray.

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Communicated by I. Rajcan.

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Ray, J.D., Morel, W., Smith, J.R. et al. Genetics and mapping of adult plant rust resistance in soybean PI 587886 and PI 587880A. Theor Appl Genet 119, 271–280 (2009). https://doi.org/10.1007/s00122-009-1036-z

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