Skip to main content

Genetics and mapping of adult plant rust resistance in soybean PI 587886 and PI 587880A

Theoretical and Applied Genetics volume 119, pages 271–280 (2009)Cite this article

Abstract

Two soybean accessions, PI 587886 and PI 587880A, previously identified as having resistance to Phakospora pachyrhizi Syd. (soybean rust, SBR) were used to create two populations (POP-1 and POP-2) segregating for SBR resistance. F2-derived F3 (F2:3) families from each population were grown in a naturally SBR-infected field in Paraguay to determine inheritance and map resistance genes. Over 6,000 plants from 178 families in POP-1 and over 5,000 plants from 160 families in POP-2 were evaluated at R5 for lesion type: immune reaction (IR), reddish-brown (RB), or tan (TAN) colored lesions. Based on the lesion type present, each F2:3 family was rated as resistant, segregating or susceptible and this classification was used to infer the F2-phenotype and genotype. For both populations, the F2 segregation ratios fit a 1:2:1 (resistant:segregating:susceptible) ratio expected for a single gene (P > 0.05). The RB lesions occurred almost exclusively in the heterozygous class, indicating incomplete dominance under the conditions of this study. Molecular markers flanking the locations of the known resistance genes were used to map the resistance gene in both populations to the Rpp1 locus. However, evaluation of PI 587886 and PI 587880A against eight P. pachyrhizi isolates indicated that the resistance allele in these two accessions was different from Rpp1. This test also demonstrated that these accessions were resistant to at least one P. pachyrhizi isolate collected in the southern US. This is the first report of using an adult plant field-screen with natural rust pressure to map SBR resistance.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Fig. 1

References

  • Arelli P, Young LD, Mengistu A (2006) Registration of high yielding and multiple disease resistant germplasm JTN5503. Crop Sci 46:2723–2724

    Article  Google Scholar 

  • Bernard RL (1967) The inheritance of pod color in soybeans. J Hered 58:165–168

    Article  Google Scholar 

  • Bernard RL, Cremeens CR (1988) Registration of ‘Williams 82’ soybean. Crop Sci 28:1027–1028

    Google Scholar 

  • Bonde MR, Nester SE, Austin CN, Stone CL, Frederick RD, Hartman GL, Miles MR (2006) Evaluation of virulence of Phakopsora pachyrhizi and P. meibomiae isolates. Plant Dis 90:708–716

    Article  Google Scholar 

  • Bromfield KR (1984) Soybean rust. Monograph No. 11, The American Phytopathological Society, St. Paul

  • Calvo ES, Kiihl RAS, Garcia A, Harada A, Hiromoto DM (2008) Two major recessive soybean genes conferring soybean rust resistance. Crop Sci 48:1350–1354

    Article  Google Scholar 

  • Chakraborty N, Curley J, Frederick RD, Hyten DL, Nelson RL, Hartman GL, Diers BW (2009) Mapping and confirmation of a new allele at Rpp1 from soybean PI 594538A conferring RB lesion type resistance to soybean rust. Crop Sci (accepted)

  • Cherry E, Peet C (1966) An efficient device for the rapid collection of fungal spores from infected plants. Phytopatholgy 56:1102–1103

    Google Scholar 

  • Fehr WR, Caviness CE (1977) Stages of soybean development. Iowa Agric Exp Stn Spec Rep 80. Iowa State University, Ames, IA

  • Garcia A, Calvo ES, Kiihl RS, Harada A, Hiromoto DM, Vieira LGE (2008) Molecular mapping of soybean rust (Phakopsora pachyrhizi) resistance genes: discovery of a novel locus and alleles. Theor Appl Genet 117:545–553

    PubMed  Article  CAS  Google Scholar 

  • Hartwig EE (1986) Identification of a fourth major gene conferring resistance to soybean rust. Crop Sci 26:1135–1136

    Google Scholar 

  • Hartwig EE, Bromfield KR (1983) Relationships among three genes conferring specific resistance to rust in soybeans. Crop Sci 23:237–239

    Google Scholar 

  • Hyten DL, Hartman GL, Nelson RL, Frederick RD, Concibido VC, Narvel JM, Cregan PB (2007) Map location of the Rpp1 locus that confers resistance to soybean rust in soybean. Crop Sci 47:837–840

    CAS  Article  Google Scholar 

  • Hyten DL, Smith JR, Frederick RD, Tuker ML, Song Q, Cregan PB (2009) Bulk segregate analysis using the GoldenGate assay to locate the Rpp3 locus that confers resistance to Phakopsora pachyrhizi (soybean rust) in soybean. Crop Sci 49:265–271

    Article  CAS  Google Scholar 

  • McLean R, Byth DE (1980) Inheritance of resistance to rust (Phakopsora pachyrhizi) in soybean. Aust J Agric Res 31:951–956

    Article  Google Scholar 

  • Melching JS, Bromfield KR, Kingsolver CH (1983) The plant pathogen containment facility at Frederick, Maryland. Plant Dis 67:717–722

    Article  Google Scholar 

  • Miles MR, Frederick RD, Hartman GL (2006) Evaluation of soybean germplasm for resistance to Phakopsora pachyrhizi. Plant Health Prog. doi:10.1094.PHP-2006=0104-01-RS

  • Miles MR, Morel W, Ray JD, Smith JR, Frederick RD, Hartman GL (2008) Adult plant evaluation of soybean accessions for resistance to Phakopsora pachyrhizi in the field and greenhouse in Paraguay. Plant Dis 92:96–105

    Article  Google Scholar 

  • Monteros MJ, Missaoui AM, Phillips DV, Walker DR, Boerma HR (2007) Mapping and confirmation of the “Hyuuga” RB lesion resistance gene for Asian soybean rust. Crop Sci 47:829–834

    CAS  Google Scholar 

  • Morel W, Scheid N, Amarilla V, Cubilla LE (2004) Soybean rust in Paraguay, evolution in the past three years. In: Moscardi J, Hoffman-Campo CB, Ferreira Saraiva O, Galerani PR, Krzyzanowski FC, Carrão-Panizzi MC (eds) Proceedings of VII World soybean research conference, IV international soybean processing and utilization conference, III Congresso Mundial de Soja (Brazilian soybean conference). Emprapa Soja, Londrina, Brazil, pp 361–364

  • Palmer RG, Pfeiffer TW, Buss GR, Kilen TC (2004) Qualitative genetics. In: Boerma HR, Specht JE (eds) Soybeans: improvement, production, and uses, 3rd edn, Agronomy Monography 16. American Society of Agronomy, Madison, pp 137–233

  • Pham TA, Miles MR, Frederick RD, Hill CB, Hartman GL (2009) Differential responses of resistant soybean entries to isolates of Phakopsora pachyrhizi. Plant Dis 93:224–228

    Article  Google Scholar 

  • Schneider RW, Hollier CA, Whitam HK, Palm ME, McKenny JM, Hernandez JR, Levy L, Devries-Paterson R (2005) First report of soybean rust caused by Phakopsora pachyrhizi in the continental United States. Plant Dis 89:774

    Article  Google Scholar 

  • Silva DCG, Yamanaka N, Brogin RL, Arias CAA, Nepomuceno AL, Di Mauro AO, Pereira SS, Nogueira LM, Passianotto ALL, Abdelnoor RV (2008) Molecular mapping of two loci that confer resistance to Asian rust in soybean. Theor Appl Genet 117:57–63

    PubMed  Article  CAS  Google Scholar 

  • Sing BB, Thapliyal PN (1977) Breeding for resistance to soybean rust in India. p. 63-65. In: Ford RE, Sinclair JB (eds) Rust of soybean-the problem and research needs. Report of a workshop held in Manila, the Philippines, 28 February–4 March 1977. Inter Agri Pub, INTSOY Series Number 12

  • Sokal RR, Rohlf FJ (1995) Biometry, 3rd edn. (W.H.) Freeman, New York, p 887

    Google Scholar 

  • Song QJ, Marek LF, Shoemaker RC, Lark KG, Concibido VC, Delannay X, Specht JE, Cregan PB (2004) A new integrated genetic linkage map of the soybean. Theor Appl Genet 109:122–128

    PubMed  Article  CAS  Google Scholar 

  • Van Ooijen JW (2006) JoinMap 4®, Software for the calculation of genetic linkage maps in experimental populations. Kyazma B.V., Wageningen, The Netherlands

  • Yates F (1934) Contingency table involving small numbers and the χ2 test. J R Stat Soc (Suppl) 1:217–235

    Article  Google Scholar 

  • Yorinori JT, Paiva WM, Frederick RD, Costamilan LM, Bertagnolli PF, Hartman GL, Godoy CV, Nunes J Jr (2005) Epidemics of soybean rust (Phakopsora pachyrhizi) in Brazil and Paraguay from 2001 to 2003. Plant Dis 89:675–677

    Article  Google Scholar 

Download references

Acknowledgments

The authors appreciate the dedicated work of the USDA-ARS personnel at Stoneville, MS, including Ms. Angelie Davis, Mr. Hans Hinrichsen, Mr. Philip Handly, Mr. Ronnie Griffin, Mr. Jeffrey Britt and Ms. Loria Courtney. In Paraguay we were ably assisted in the field and laboratory by Ms. Noelia Bogado, Mr. Ismael Morel, Mr. Francisco Saucedo, Mr. Claudio Vigo, Ms. Diana Bado, Ms. Sergia Portillo, and Ms. Nidia Benitez. The Paraguay based portion of this work was critically facilitated by Mr. Luis Enrique Robledo of DIA and Mr. Luis E. Cubilla of CAPECO. Seed production in Puerto Rico by Ricardo Goenaga and Carlos Torres of the USDA-ARS Tropical Agriculture Research Station was critical for the timely completion of this project. We also appreciate the efforts of Ms. Christine Stone for assistance with the inoculations at the FDWSRU. This research could not have been accomplished without these combined efforts.

Author information

Affiliations

  1. USDA-ARS, Crop Genetics and Production Research Unit, Stoneville, MS, 38776, USA

    Jeffery D. Ray & James R. Smith

  2. Centro Regional de Investigación Agrícola, Capitán Miranda, Itapúa, Paraguay

    Wilfrido Morel

  3. USDA-ARS, Foreign Disease-Weed Science Research Unit, Ft. Detrick, MD, 21702, USA

    Reid D. Frederick

  4. USDA-ARS, National Soybean Research Center, University of Illinois, Urbana, IL, 61801, USA

    Monte R. Miles

Authors
  1. Jeffery D. Ray
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wilfrido Morel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. James R. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Reid D. Frederick
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Monte R. Miles
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jeffery D. Ray.

Additional information

Communicated by I. Rajcan.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ray, J.D., Morel, W., Smith, J.R. et al. Genetics and mapping of adult plant rust resistance in soybean PI 587886 and PI 587880A. Theor Appl Genet 119, 271–280 (2009). https://doi.org/10.1007/s00122-009-1036-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-009-1036-z

Keywords

  • Simple Sequence Repeat Marker
  • Lesion Type
  • Soybean Accession
  • Soybean Rust
  • Polymorphic Simple Sequence Repeat Marker