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Isolation and characterisation of an lpa (low phytic acid) mutant in common bean (Phaseolus vulgaris L.)

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Abstract

Phytic acid is considered as one of the major antinutritional compounds in cereal and legume seeds. The development of lpa (low phytic acid) grains, resulting in increased mineral cation availability, is considered a major goal in the improvement of the nutritional quality of seed crops, especially those largely consumed in developing countries. From a mutagenised population of common bean we isolated a homozygous lpa mutant line (lpa-280-10) showing, compared to wild type, a 90% reduction of phytic acid, a 25% reduction of raffinosaccharides and a much higher amount of free or weakly bound iron cations in the seed. Genetic analysis showed that the lpa character is due to a recessive mutation that segregates in a monogenic, Mendelian fashion. Germination tests performed using varying ageing or stress conditions, clearly showed that the bean line lpa-280-10 has a better germination response than the wild type. These data, together with those obtained from 2 years of agronomic trials showing that the mutant seed yield is close to that of its parents and other evidence, indicate that the new lpa-280-10 mutation might be the first devoid of visible macroscopic negative effects in plants, pods and seeds.

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Acknowledgments

We are grateful to Dr. Sonia Mazzamurro for the screening work she carried out during her thesis stage in the E.N. laboratory, to Dr. Antonio Dell’Aquila for his help in germination test analysis and for his comments to the manuscript, and to Dr. Roberto Pilu for helpful discussions. Technical support by Mrs. Gloria Daminati and Mr. Rommel Ocampo Romero Ivan are acknowledged. Dr. Marzia Fileppi was supported by a research grant from CRA. This research was partially supported by Ministry of Agricultural Alimentary and Forest Politics with funds released by C.I.P.E (Resolution 17/2003) to B.C. and F.S. and by Ministry for University and Research (PRIN 2006) to E.N.

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Authors and Affiliations

  1. CRA, Unità di Ricerca per l’Orticoltura, Montanaso Lombardo, Lodi, Italy

    Bruno Campion & Marzia Fileppi

  2. Istituto di Biologia e Biotecnologia Agraria, CNR, Milan, Italy

    Francesca Sparvoli, Giovanni Tagliabue, Incoronata Galasso, Marzia Fileppi & Roberto Bollini

  3. Dipartimento di Genetica e Microbiologia, Università di Pavia, Via Ferrata 1, Pavia, Italy

    Enrico Doria & Erik Nielsen

Authors
  1. Bruno Campion
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  2. Francesca Sparvoli
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  3. Enrico Doria
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  4. Giovanni Tagliabue
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  5. Incoronata Galasso
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  6. Marzia Fileppi
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  7. Roberto Bollini
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  8. Erik Nielsen
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Correspondence to Erik Nielsen.

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Communicated by D. Lightfoot.

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122_2009_975_MOESM1_ESM.doc

Plant materials used in this work and breeding path followed to obtain the “lectin-free + low phytic acid ” bean lines. (DOC 59 kb)

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Campion, B., Sparvoli, F., Doria, E. et al. Isolation and characterisation of an lpa (low phytic acid) mutant in common bean (Phaseolus vulgaris L.). Theor Appl Genet 118, 1211–1221 (2009). https://doi.org/10.1007/s00122-009-0975-8

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