Skip to main content
SpringerLink
Log in

Mapping and validation of quantitative trait loci for resistance to Cercospora zeae-maydis infection in tropical maize (Zea mays L.)

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Breeding for resistance to gray leaf spot, caused by Cercospora zeae-maydis (Cz) is paramount for many maize environments, in particular under warm and humid growing conditions. In this study, we mapped and characterized quantitative trait loci (QTL) involved in the resistance of maize against Cz. We confirmed the impact of the QTL on disease severity using near-isogenic lines (NILs), and estimated their effects on three major agronomic traits using their respective near isogenic hybrids (NIHs), which we obtained by crossing the NILs with an inbred from a complementary heterotic pool. We further validated three of the four QTL that were mapped using the Multiple Interval Mapping approach and showed LOD values >2.5. NILs genotype included all combinations between favorable alleles of the two QTL located in chromosome 1 (Q 1 in bin 1.05 and Q 2 in bin 1.07), and the allele in chromosome 3 (Q 3 in bin 3.07). Each of the three QTL separately significantly reduced the severity of Cz. However, we found an unfavorable epistatic interaction between Q 1 and Q 2: presence of the favorable allele at one of the QTL allele effectively nullified the effect of the favorable allele at the other. In contrast, the interaction between Q 2 and Q 3 was additive, promoting the reduction of the severity to a greater extent than the sum of their individual effects. When evaluating the NIH we found significant individual effects for Q 1 and Q 3 on gray leaf spot severity, for Q 2 on stalk lodging and grain yield, and for Q 3 on grain moisture and stalk lodging. We detected significant epitasis between Q 1 and Q 2 for grain moisture and between Q 1 and Q 3 for stalk lodging. These results suggest that the combination of QTL impacts the effectiveness of marker-assisted selection procedures in commercial product development programs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Abler BS, Edwards M, Stuber CW (1991) Isoenzymatic identification of quantitative trait loci in crosses of elite maize inbreds. Crop Sci 31(2):267–274

    CAS  Google Scholar 

  • Austin DF, Lee M, Veldboom LR, Hallauer AR (2000) Genetic mapping in maize with hybrid progeny across testers and generations: grain yield and grain moisture. Crop Science 40:30–39

    Google Scholar 

  • Bernacchi D, Beck-Bunn T, Eshed Y, Lopes J, Petiard V, Uhlig J, Zamir D, Tanksley S (1998) Advanced backcross QTL analysis in tomato. I. Identification of QTL for traits of agronomic importance from Lycopersicon hirsutum. Theor Appl Genet 97:381–397

    Article  CAS  Google Scholar 

  • Bubeck DM, Goodman MM, Beavis WD, Grant D (1993) Quantitative trait loci controlling resistance do gray leaf spot in maize. Crop Sci 33:838–847

    Google Scholar 

  • Carlborg O, Haley CS (2004) Epistasis: too often neglected in complex trait studies? Nature 5:618–625

    CAS  Google Scholar 

  • Clements MJ, Dudley JW, White DG (2000) Quantitative trait loci associated with resistance to gray leaf spot of corn. Phytopathology 90:1018–1025

    Article  PubMed  CAS  Google Scholar 

  • Coates ST, White DG (1998) Inheritance of resistance to gray leaf spot in crosses involving selected resistant inbred lines of corn. Phytopathology 88:972–982

    Article  PubMed  CAS  Google Scholar 

  • Collard BCY, Jahufer MZZ, Brouwer JB, Pang ECK (2005) An Introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: the basic concepts. Euphytica 142:169–196

    Article  CAS  Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA mini preparation: version II. Plant Mol Biol Rep 1:19–21

    Article  CAS  Google Scholar 

  • Dodd JL (1980) The role of plant stresses in the development of maize stalk rots. Plant Dis 64:533–537

    Google Scholar 

  • Doebley J, Stec A, Gustus C (1995) Teosinte branched 1 and the origin of maize: evidence for epistasis and the evolution of dominance. Genetics 141:333–346

    PubMed  CAS  Google Scholar 

  • Eberhart SA, Russell WA (1966) Stability parameters for comparing varieties. Crop Sci 6:36–40

    Google Scholar 

  • Eshed Y, Zamir D (1996) Less-than-additive epistatic interactions of quantitative trait loci in tomato. Genetics 143:1807–1817

    PubMed  CAS  Google Scholar 

  • Gardiner JM, Coe EH, Melia-Hancock S, Hoisington DA, Chao S (1993) Development of a core RFLP map in maize using an immortalized F2 population. Genetics 134:917–930

    PubMed  CAS  Google Scholar 

  • Glover KD, Wang D, Arelli PR, Carlson SR, Cianzio SR, Diers BW (2004) Near isogenic lines confirm a soybean cyst nematode resistance gene from PI 88788 on linkage group. J Crop Sci 44:936–941

    Google Scholar 

  • Gordon GS, Bartsch M, Matties I, Gevers HO, Lipps PE, Pratt RC (2004) Linkage of molecular markers to Cercospora zeae-maydis resistance in maize. Crop Sci 44:628–636

    CAS  Google Scholar 

  • Hittalmani S, Shashidhar HE, Bagali PG, Huang N, Sidhu JS, Singh VP, Khush GS (2002) Molecular mapping of quantitative trait loci for plant growth, yield and yield related traits across three diverse locations in a doubled haploid rice population. Euphytica 125:207–214

    Article  CAS  Google Scholar 

  • Jensen RC (1992) A general mixture model for mapping quantitative trait loci by using molecular markers. Theor Appl Genet 85:252–260

    Google Scholar 

  • Jensen RC (1993) Interval mapping of multiple quantitative trait loci. Genetics 135:205–211

    Google Scholar 

  • Kao C, Zeng Z, Teasdale RD (1999) Multiple interval mapping for quantitative trait loci. Genetics 152:1203–1216

    PubMed  CAS  Google Scholar 

  • Lander ESP, Abrahamson GJ, Barlow A, Daly M, Lincoln S, Newburg L, (Whitehead Institute) (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Lehmensiek A, Esterhuizen AM, van Staden C (2001) Genetic mapping of gray leaf spot (GLS) resistance genes in maize. Theor Appl Genet 103:797–803

    Article  CAS  Google Scholar 

  • Li Z, Pinson SRM, Park WD, Paterson AH, Stansel JW (1997) Epistasis for three grain yield components in rice (Oryza sativa L.). Genetics 145:453–465

    PubMed  CAS  Google Scholar 

  • Melchinger AE, Utz HF, Schön CC (1998) Quantitative trait locus (QTL) mapping using different testers and independent population samples in maize reveals low power of QTL detection and large bias in estimates of QTL effects. Genetics 149:383–403

    PubMed  CAS  Google Scholar 

  • Pedrosa MG (2002) Mapeamento genético para resistência à Cercosporiose, mancha de feosféria e ferrugem comum na cultura do milho. Dissertação de mestrado. Universidade Federal de Uberlândia – MG.102 p

  • Saghai Maroof MA, Yue YG, Xiang ZX, Stromberg EL, Rufener GK (1996) Identification of quantitative trait loci controlling resistance to gray leaf spot disease in maize. Theor Appl Genet 93(4):539–546

    Article  Google Scholar 

  • Van Berloo R, Aalbers H, Werkman A, Niks RE (2001) Resistance QTL confirmed through development of QTL-NILs for barley leaf rust resistance. Molec Breed 8:187–195

    Article  Google Scholar 

  • Varona L, Raya LG, Rauw WM, Noguera JL (2001) Can F2 mapping experiments be used to detect epistatic interactions? 7th Quantitative trait locus mapping and marker-assisted selection workshop. Universidad Politécnica de Valencia, Spain

  • Wang S, Basten C, Zeng Z-B (1999) Windows QTL cartographer. Department of Statistics, North Carolina State University, Raleigh, NC, USA http://statgen.ncsu.edu/qtlcart/WQTLCart.htm)

  • Ward JMJ, Stromberg EL, Nowell DC, Nutter FW Jr (1999) Gray leaf spot—a disease of global importance in maize production. Plant Dis 83:884–895

    Article  Google Scholar 

  • Wisser RJ, Balint-Kurti PJ, Nelson RJ (2006) The genetic architecture of disease resistance in maize: a synthesis of published studies. Phytopathology 96:120–129

    Article  PubMed  CAS  Google Scholar 

  • Zeng ZB (1993) Theoretical basis for separation of multiple linked gene effects in mapping quantitative trait loci. Proc Natl Acad Sci USA 90:10972–10976

    Article  PubMed  CAS  Google Scholar 

  • Zeng ZB (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1466

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We thank Manuel Oyervides and Humberto Gutierrez from Monsanto Company for their support; Rogério Alvez de Andrade, Mike Kerns and Ivani Pozar Otsuk for suggestions and help; John Schoper and Pedro Nurmberg for reviewing the manuscript. We are also grateful to Osmar de Souza and Ricardo Piccinato.We also thank the Universidade Federal de Uberlândia (UFU) and specifically Prof. Dr. Luíz Ricardo Goulart Filho.

Author information

Authors and Affiliations

  1. Monsanto do Brasil Ltda, Rod. BR452-Km, Uberlândia, MG, Brazil

    Gilberto Pozar & Heyder Diniz Silva

  2. Monsanto Company, 3302 S.E. Convenience Blvd, Ankeny, IA, USA

    David Butruille & Zoe Patterson McCuddin

  3. Universidade Federal de Uberlândia (UFU), Uberlândia, MG, Brazil

    Julio Cesar Viglioni Penna

Authors
  1. Gilberto Pozar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. David Butruille
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Heyder Diniz Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zoe Patterson McCuddin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Julio Cesar Viglioni Penna
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gilberto Pozar.

Additional information

Communicated by M. Bohn.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pozar, G., Butruille, D., Silva, H.D. et al. Mapping and validation of quantitative trait loci for resistance to Cercospora zeae-maydis infection in tropical maize (Zea mays L.). Theor Appl Genet 118, 553–564 (2009). https://doi.org/10.1007/s00122-008-0920-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-008-0920-2

Keywords

Search

Navigation