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Dynamic expression of nine QTLs for tiller number detected with single segment substitution lines in rice

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Abstract

Nine single segment substitution lines (SSSLs) in rice, which contain quantitative trait loci (QTLs) for tiller number on substituted segments detected in previous studies, were selected as materials to analyse dynamic expression of the QTLs in this study. These SSSLs and their recipient parent, Hua-jing-xian 74 (HJX74), were grown in four different environments and were measured for tiller number at nine different growth stages. An indirect methodology was applied in QTL mapping through analyzing multi-environment phenotypic data. Dynamics of three types of effects (including total effect, main effect, and QE interaction effect) of QTLs was released. It was shown that nine QTLs exhibited statistically significant effects only at certain stages. Effects of a QTL, although insignificant at certain stages, displayed dynamic change with the growth of rice plants. Two common features of nine QTLs were detected, one is no expression within 7 days after transplanting, and the other is opposite expression existed during the whole growth period. Nine QTLs largely focused on expression in certain stages, and accordingly were suggested to partition into three types, expression in prophase, both in prophase and in anaphase, and evenly during the whole stage. It may be reasonable explanation that dynamics of main effects of QTLs are likely due to gene expression selectly at certain times, while dynamics of QE interaction effects of QTLs might attribute to the subrogation of environmental factors. Examination of the association between QE interaction effect and specified environmental factors across stages may provide useful information on how an environmental factor regulates QTL expression.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (30330370 and 30830074).

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Correspondence to Guiquan Zhang.

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Communicated by M. Cooper.

G. Liu and R. Zeng contributed equally to this work.

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Liu, G., Zeng, R., Zhu, H. et al. Dynamic expression of nine QTLs for tiller number detected with single segment substitution lines in rice. Theor Appl Genet 118, 443–453 (2009). https://doi.org/10.1007/s00122-008-0911-3

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