We have constructed a linkage map of bunching onion (Allium fistulosum L., 2n = 16) using an F2 population of 225 plants. The map consists of 17 linkage groups with 212 bunching onion SSR markers and 42 bulb onion (A. cepa L.) SSR, InDel, CAPS or dCAPS markers, covering 2,069 cM. This is the first report of a linkage map mainly based on SSR markers in the genus Allium. With the 103 anchor markers [81 bunching onion SSRs, 11 bulb onion SSRs and 11 bulb onion non-SSRs (1 InDel, 9 CAPSs and 1 dCAPS)] whose chromosome assignments were identified in A. cepa and/or A. fistulosum, via the use of several kinds of Allium alien addition lines, 16 of the 17 linkage groups were connected to the 8 basic chromosomes of A. cepa.
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We are grateful to Ms. K. Tanaka, K. Wakita and S. Negoro for the technical assistance. This work was supported by the project “Green Technology Project (DM-1604)”, Ministry of Agriculture, Forestry and Fisheries, Japan and Grant-in-Aid for Young Scientists (B) (19780010), Japan Society for the Promotion of Science. The authors wish to thank Ms. N. Matsubara, Y. Kousabara and M. Anraku (former students in Yamaguchi University) for their contributions to this study.
Communicated by M. Xu.
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Tsukazaki, H., Yamashita, K., Yaguchi, S. et al. Construction of SSR-based chromosome map in bunching onion (Allium fistulosum). Theor Appl Genet 117, 1213 (2008). https://doi.org/10.1007/s00122-008-0849-5
- Linkage Group
- Simple Sequence Repeat Marker
- Cleave Amplify Polymorphic Sequence
- Bulb Onion
- Cleave Amplify Polymorphic Sequence Marker