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Mapping of isolate-specific QTLs for clubroot resistance in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

Theoretical and Applied Genetics volume 117, pages 759–767 (2008)Cite this article

Abstract

A number of clubroot resistant (CR) Chinese cabbage cultivars have been developed in Japan using resistant genes from CR European fodder turnips (B. rapa ssp. rapifera). Clubroot resistance in European fodder turnips are known to be controlled by the combined action of several dominant resistance genes. We have developed three Chinese cabbage clubroot-resistant doubled haploid (DH) lines—T136-8, K10, and C9—which express resistance in different manners against two isolates of Plasmodiophora brassicae, M85 and K04. Depending on the isolates, we identified two CR loci, CRk and CRc. CRk was identified by quantitative trait loci (QTL) analysis of an F2 population derived from a cross between K10 and Q5. This locus showed resistance to both isolates and is located close to Crr3 in linkage group R3. The other locus, CRc was identified by QTL analysis of an F2 population derived from a cross between C9 and susceptible DH line, 6R. This locus was mapped to linkage group R2 and is independent from any published CR loci. We developed sequence-tagged site markers linked to this locus.

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Acknowledgments

The authors thank Dr. S. Matsumoto of National Institute of Vegetable and Tea Science (NIVTS) for offering information on the SSR markers of B. rapa (BRMS).

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Affiliations

  1. Takii Plant Breeding and Experiment Station, Konan, Shiga, 520-3231, Japan

    Koji Sakamoto & Atsuo Saito

  2. Division of Gene Research, Shinshu University, Ueda, Nagano, 386-8567, Japan

    Nobuaki Hayashida

  3. Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, 386-8567, Japan

    Goro Taguchi

  4. Nagano Vegetable and Ornamental Crops Experiment Station, Matsushiro, Nagano, 381-1211, Japan

    Etsuo Matsumoto

Authors
  1. Koji Sakamoto
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  2. Atsuo Saito
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  3. Nobuaki Hayashida
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  4. Goro Taguchi
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  5. Etsuo Matsumoto
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Corresponding author

Correspondence to Koji Sakamoto.

Additional information

Communicated by C. F. Quiros.

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Sakamoto, K., Saito, A., Hayashida, N. et al. Mapping of isolate-specific QTLs for clubroot resistance in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Theor Appl Genet 117, 759–767 (2008). https://doi.org/10.1007/s00122-008-0817-0

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  • DOI: https://doi.org/10.1007/s00122-008-0817-0

Keywords

  • Quantitative Trait Locus
  • Amplify Fragment Length Polymorphism
  • Simple Sequence Repeat Marker
  • Double Haploid
  • Chinese Cabbage