Abstract
The Rc locus regulates pigmentation of the rice bran layer, and selection for the rc allele (white pericarp) occurred during domestication of the crop. White bran is now ubiquitous among cultivated varieties throughout rice growing regions of the world. We identified a new allele that arose by natural mutation within the rc pseudogene of the cultivar ‘Wells’. The mutation restored the reading frame of the gene, and reverted the bran layer pigmentation to red (wild-type). By sequencing the Rc locus in plants derived from red seeds, and linkage analysis in a segregating population, we were able to demonstrate that mutation within rc resulted in the new, dominant, wild-type allele Rc-g.
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References
Arkansas State Plant Board (2002) Official standards for seed certification in Arkansas. Circular 15
Furukawa T, Maekawa M, Oki T, Suda I, Iida S, Shimada H, Takamure I, Kadowaki K-i (2007) The Rc and Rd genes are involved in proanthocyanidin synthesis in rice pericarp. Plant J 49:91–102
Gealy DR, Tai TH, Sneller CH (2002) Identification of red rice, rice, and hybrid populations using microsatellite markers. Weed Sci 50:333–339
Gramene (2007) Cold Spring Harbor Laboratory and Cornell University, USA. http://www.gramene.org. Cited 26 December 2007
Ji H-S, Chu S-H, Jiang W, Cho Y-I, Hahn J-H, Eun M-Y, McCouch SR, Koh H-J (2006) Characterization and mapping of a shattering mutant in rice that corresponds to a block of domestication genes. Genetics 173:995–1005
Ling WH, Cheng QX, Ma J, Wang T (2001) Red and black rice decrease atherosclerotic plaque formation and increase antioxidant status in rabbits. J Nutr 131:1421–1426
Moldenhauer KAK, Lee FN, Bernhardt JL, Norman RJ, Slaton NA, Wilson CE, Anders MM, Cartwright RD, Blocker MM (2007) Registration of ‘Wells’ rice. Crop Sci 47:442–443
Slaton N, Moldenhauer K, Gibbons J, Blocker M, Wilson C, Dilday R, Robinson J, Koen B (2000) Grain characteristics of rice varieties. University of Arkansas, Cooperative Extension Service, Rice Information Bulletin, No. 146
Sweeney MT, Thomson MJ, Pfeil BE, McCouch S (2006) Caught red-handed: Rc encodes a basic helix-loop-helix protein conditioning red pericarp in rice. Plant Cell 18:283–294
Wilson C (2002) Arkansas rice acreage by variety survey. Proceedings of the 30th Rice Technical Working Group, New Orleans, LA
Acknowledgments
The authors extend thanks to the following people for contributions to this work: Dr. Leopoldo Estorninos, Dr. David Gealy, Melissa Jia, Gordon Miller, and Ronnie Sherman.
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Communicated by T. Sasaki.
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Brooks, S.A., Yan, W., Jackson, A.K. et al. A natural mutation in rc reverts white-rice-pericarp to red and results in a new, dominant, wild-type allele: Rc-g . Theor Appl Genet 117, 575–580 (2008). https://doi.org/10.1007/s00122-008-0801-8
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DOI: https://doi.org/10.1007/s00122-008-0801-8