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Molecular mapping of soybean rust (Phakopsora pachyrhizi) resistance genes: discovery of a novel locus and alleles

Theoretical and Applied Genetics volume 117, Article number: 545 (2008) Cite this article

Abstract

Soybean production in South and North America has recently been threatened by the widespread dissemination of soybean rust (SBR) caused by the fungus Phakopsora pachyrhizi. Currently, chemical spray containing fungicides is the only effective method to control the disease. This strategy increases production costs and exposes the environment to higher levels of fungicides. As a first step towards the development of SBR resistant cultivars, we studied the genetic basis of SBR resistance in five F2 populations derived from crossing the Brazilian-adapted susceptible cultivar CD 208 to each of five different plant introductions (PI 200487, PI 200526, PI 230970, PI 459025, PI 471904) carrying SBR-resistant genes (Rpp). Molecular mapping of SBR-resistance genes was performed in three of these PIs (PI 459025, PI 200526, PI 471904), and also in two other PIs (PI 200456 and 224270). The strategy mapped two genes present in PI 230970 and PI 459025, the original sources of Rpp2 and Rpp4, to linkage groups (LG) J and G, respectively. A new SBR resistance locus, rpp5 was mapped in the LG-N. Together, the genetic and molecular analysis suggested multiple alleles or closely linked genes that govern SBR resistance in soybean.

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Acknowledgments

The authors would like to thank Marcelo S. Baço and Adriano A. Marino for technical assistance with plant analysis and the anonymous reviewers who contributed to improvement of the manuscript. This project was financially supported by FAPEMAT (Fundação de Apoio a Pesquisa do Estado de Mato Grosso) and FINEP (Financiadora de Estudos e Projetos – Ação Estruturante Transversal). Alexandre Garcia received a scholarship from Fundação Araucária.

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Affiliations

  1. State University of Londrina (UEL), Rod. Celso Garcia Cid Km 380, Cx. Postal 6001, CEP 86051-990, Londrina, PR, Brazil

    Alexandre Garcia

  2. Tropical Melhoramento e Genética Ltda (TMG), Rod. Celso Garcia Cid Km 87, Cx. Postal 387, CEP 86183-600, Cambé, PR, Brazil

    Alexandre Garcia, Éberson Sanches Calvo, Romeu Afonso de Souza Kiihl & Arlindo Harada

  3. Fundação de Apoio a Pesquisa Agropecuária do Estado de Mato Grosso (Fundação MT), Av. Antônio Teixeira dos Santos 1559, Cx. Postal 79, CEP 78750-000, Rondonópolis, MT, Brazil

    Dario Minoru Hiromoto

  4. Instituto Agronômico do Paraná (IAPAR), Rod. Celso Garcia Cid km 375, Cx. Postal 481, CEP 86047-902, Londrina, PR, Brazil

    Luiz Gonzaga Esteves Vieira

Authors
  1. Alexandre Garcia
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  2. Éberson Sanches Calvo
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  3. Romeu Afonso de Souza Kiihl
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  4. Arlindo Harada
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  5. Dario Minoru Hiromoto
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  6. Luiz Gonzaga Esteves Vieira
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Correspondence to Éberson Sanches Calvo.

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Communicated by I. Rajcan.

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Garcia, A., Calvo, É.S., de Souza Kiihl, R.A. et al. Molecular mapping of soybean rust (Phakopsora pachyrhizi) resistance genes: discovery of a novel locus and alleles. Theor Appl Genet 117, 545 (2008). https://doi.org/10.1007/s00122-008-0798-z

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  • DOI: https://doi.org/10.1007/s00122-008-0798-z

Keywords

  • Linkage Group
  • Rust Resistance
  • Rust Resistance Gene
  • Bulk Segregant Analysis
  • Allelism Test