Identification of defense-related genes in rice responding to challenge by Rhizoctonia solani
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Rice sheath blight, caused by Rhizoctonia solani is one of the major diseases of rice. The pathogen infects rice plants directly through stomata or using lobate appressoria and hyphal masses called infection cushions. The infection structures were normally found at 36 h post-inoculation. During infection, the pathogenesis-related genes, PR1b and PBZ1 were induced in rice plants. To identify rice genes induced early in the defense response, suppression subtractive hybridization (SSH) was used to generate a cDNA library enriched for transcripts differentially expressed during infection by R. solani. After differential screening by membrane-based hybridization and subsequent confirmation by reverse Northern blot analysis, selected clones were sequenced. Fifty unique cDNA clones were found and assigned to five different functional categories. Most of the genes were not previously identified as being induced in response to pathogens. We examined expression of 100 rice genes induced by infection with Magnaporthe grisea, Xanthomonas oryzae pv. oryze (Xoo) and X. oryzae pv. oryzicola (Xooc). Twenty-five of them were found to be differentially expressed after the sheath blight infection, suggesting overlap of defense responses to different fungal and bacterial pathogens infection.
KeywordsSuppression Subtractive Hybridization Rice Blast Translationally Control Tumor Protein Sheath Blight Bacterial Leaf Blight
This research was supported by National Basic Research Program of China (Project No. 2006CB1019001), Commonweal Research Program of Agricultural Science of China (Project No. nyhyzx07-049) and Natural Science Foundation of Fujian Province of China (Project No. 2003F008). We thank Dr. Daniel Ebbole of Texas A & M University for his critical reading of the manuscript.
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