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Theoretical and Applied Genetics

, Volume 116, Issue 4, pp 501–516 | Cite as

Identification of defense-related genes in rice responding to challenge by Rhizoctonia solani

  • Chang-Jiang Zhao
  • Ai-Rong Wang
  • Yu-Jun Shi
  • Liu-Qing Wang
  • Wen-De Liu
  • Zong-Hua WangEmail author
  • Guo-Dong LuEmail author
Original Paper

Abstract

Rice sheath blight, caused by Rhizoctonia solani is one of the major diseases of rice. The pathogen infects rice plants directly through stomata or using lobate appressoria and hyphal masses called infection cushions. The infection structures were normally found at 36 h post-inoculation. During infection, the pathogenesis-related genes, PR1b and PBZ1 were induced in rice plants. To identify rice genes induced early in the defense response, suppression subtractive hybridization (SSH) was used to generate a cDNA library enriched for transcripts differentially expressed during infection by R. solani. After differential screening by membrane-based hybridization and subsequent confirmation by reverse Northern blot analysis, selected clones were sequenced. Fifty unique cDNA clones were found and assigned to five different functional categories. Most of the genes were not previously identified as being induced in response to pathogens. We examined expression of 100 rice genes induced by infection with Magnaporthe grisea, Xanthomonas oryzae pv. oryze (Xoo) and X. oryzae pv. oryzicola (Xooc). Twenty-five of them were found to be differentially expressed after the sheath blight infection, suggesting overlap of defense responses to different fungal and bacterial pathogens infection.

Keywords

Suppression Subtractive Hybridization Rice Blast Translationally Control Tumor Protein Sheath Blight Bacterial Leaf Blight 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was supported by National Basic Research Program of China (Project No. 2006CB1019001), Commonweal Research Program of Agricultural Science of China (Project No. nyhyzx07-049) and Natural Science Foundation of Fujian Province of China (Project No. 2003F008). We thank Dr. Daniel Ebbole of Texas A & M University for his critical reading of the manuscript.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Chang-Jiang Zhao
    • 1
  • Ai-Rong Wang
    • 1
  • Yu-Jun Shi
    • 1
  • Liu-Qing Wang
    • 1
  • Wen-De Liu
    • 1
  • Zong-Hua Wang
    • 1
    Email author
  • Guo-Dong Lu
    • 1
    Email author
  1. 1.The Key Laboratory of Biopesticide and Chemical Biology, The Ministry of Education, and Functional Genomics CenterFujian Agriculture and Forestry UniversityFuzhouChina

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