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Genetic mapping of clubroot resistance genes in oilseed rape

Theoretical and Applied Genetics volume 116, Article number: 363 (2008) Cite this article

Abstract

Clubroot caused by the obligate biotrophic protist Plasmodiophora brassicae is a major disease of Brassica species. Clubroot resistances introduced from B. oleracea var. ‘Böhmerwaldkohl’ and resistance from B. rapa ECD-04 were genetically mapped in oilseed rape (B. napus L.). A doubled haploid (DH) population of rape seed was developed by crossing a resistant DH-line derived from a resynthesized B. napus with the susceptible cultivar ‘Express’. The DH population was tested in the greenhouse against seven P. brassicae isolates showing low and high virulence toward B. oleracea or/and B. rapa. DH-lines with highest or lowest disease scores were used in a bulked segregant analysis (BSA), and 43 polymorphic AFLPs were identified. A genetic map of the whole genome was constructed using 338 AFLP and 156 anchored SSR markers. Nineteen QTL were detected on chromosomes N02, N03, N08, N13, N15, N16 and N19 giving resistance to seven different isolates. Race-specific effects were observed for all QTL, none of the QTL conferred resistance to all isolates. The phenotypic variance explained by the respective QTL ranged between 10.3 and 67.5%. All QTL could be assigned to both ancestral genomes of B. napus. In contrast to previous reports, a clear differentiation into major QTL from B. rapa and minor QTL from B. oleracea could not be found. Composite interval mapping confirmed the linkage relationships determined by BSA, thus demonstrating that markers for oligogenic traits can be selected by merely testing the distributional extremes of a segregating population.

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Acknowledgments

The authors thank I. Happstadius (Svalöv-Weibull AB), G. Thomas (INRA LeRheu), H. Busch (DSV Thüle) and G. Korporal (NPZ Hohenlieth) for kindly providing the P. brassicae isolates. Financial support by the “Stiftung Schleswig-Holsteinische Landschaft” is gratefully acknowledged.

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Affiliations

  1. Plant Breeding Institute, Christian-Albrechts-University of Kiel, Olshausenstr. 40, 24098, Kiel, Germany

    S. Werner & C. Jung

  2. Institute of Biology/Applied Genetics, Free University of Berlin, Albrecht-Thaer-Weg 6, 14195, Berlin, Germany

    E. Diederichsen

  3. Norddeutsche Pflanzenzucht, Hans-Georg Lembke KG, Hohenlieth, 24363, Holtsee, Germany

    M. Frauen

  4. Saaten-Union Resistenzlabor GmbH, Hovedisser Str. 92, 33818, Leopoldshöhe, Germany

    J. Schondelmaier

  5. Institut für Pflanzenbau und Pflanzenzüchtung, Christian-Albrechts-Universität zu Kiel, Olshausenstraße 40, 24118, Kiel, Germany

    C. Jung

Authors
  1. S. Werner
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  2. E. Diederichsen
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  3. M. Frauen
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  4. J. Schondelmaier
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  5. C. Jung
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Corresponding author

Correspondence to C. Jung.

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Communicated by C. F. Quiros.

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Werner, S., Diederichsen, E., Frauen, M. et al. Genetic mapping of clubroot resistance genes in oilseed rape. Theor Appl Genet 116, 363 (2008). https://doi.org/10.1007/s00122-007-0674-2

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  • DOI: https://doi.org/10.1007/s00122-007-0674-2

Keywords

  • Simple Sequence Repeat Marker
  • Doubled Haploid
  • Chinese Cabbage
  • Oilseed Rape
  • Doubled Haploid Line