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Evaluation of genes from eIF4E and eIF4G multigenic families as potential candidates for partial resistance QTLs to Rice yellow mottle virus in rice

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Abstract

QTLs for partial resistance to Rice yellow mottle virus (RYMV) in rice were mapped in two populations of doubled-haploid lines (DHLs) and recombinant inbred lines (RILs) derived from the same cross but evaluated for different resistance criteria (virus content and symptom severity). An integrative map was used to compare the two genetic maps and a global analysis of both populations was performed. Most of the QTLs previously identified in DHL population were confirmed with increased significance and precision. As many recent studies evidenced the role of eukaryotic translation initiation factors (eIF) of 4E and 4G families in plant susceptibility to RNA viruses, we checked if these genes co-locate with QTLs of resistance to RYMV. Their systematic in silico identification was carried out on the rice genome and their physical locations were compared to QTL positions on the integrative map. In order to confirm or not the co-locations observed, the analysis was completed by evaluation of near-isogenic lines, QTL fine mapping and sequencing of candidate genes. Three members from eIF4G family could be retained as reliable candidates whereas eIF4E genes, commonly found to govern resistances in other plant/virus interactions, were discarded. Together with the recent identification of an eIF(iso)4G as a major resistance gene, data suggests an important role of genes from eIF4G family in rice resistance to RYMV but does not exclude the contribution of factors different from the translation initiation complex.

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Acknowledgments

This work was supported in part by the GENOPLANTE program TRANSVIR. Arnaud Boisnard was supported by a fellowship from the French Ministry of Research and Deless Thiémélé by a fellowship from the French-speaking University network (AUF). We gratefully acknowledge Thierry Mathieu and Sophie Perez for plant care and Nour Ahmadi for having provided us genotypes data of RIL population. Stéphane Jouannic’s help with protein sequence alignment and phylogenetic tree was greatly appreciated. We thank Romain Guyot and Mathias Lorieux for critical reviews of the manuscript.

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Authors and Affiliations

  1. UMR 5096, Laboratoire Génome et Développement des Plantes, IRD/CNRS/Université de Perpignan, BP 64501, 34394, Montpellier CEDEX 5, France

    Arnaud Boisnard, Laurence Albar, Deless Thiéméle, Myriam Rondeau & Alain Ghesquière

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  1. Arnaud Boisnard
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  2. Laurence Albar
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  3. Deless Thiéméle
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  4. Myriam Rondeau
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  5. Alain Ghesquière
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Correspondence to Laurence Albar.

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Communicated by Y. Xue.

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Boisnard, A., Albar, L., Thiéméle, D. et al. Evaluation of genes from eIF4E and eIF4G multigenic families as potential candidates for partial resistance QTLs to Rice yellow mottle virus in rice. Theor Appl Genet 116, 53–62 (2007). https://doi.org/10.1007/s00122-007-0646-6

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