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Natural variation of Arabidopsis thaliana reveals that aluminum resistance and proton resistance are controlled by different genetic factors

Theoretical and Applied Genetics volume 115, pages 709–719 (2007)Cite this article

Abstract

Root growth of Arabidopsis thaliana is inhibited by proton rhizotoxicity in low ionic strength media when the pH of the medium is lower than 5.0. QTL analysis at pH 4.7 revealed that two major QTLs on chromosome 2 and 5 and an additional six epistatic interacting loci pairs control proton resistance in the Ler/Col recombinant inbred population. These genetic factors are independently associated with proton resistance in comparison to the known Al resistant QTL and epistases detected in the same RI population at 4 μM Al at pH 5.0. This indicates that different genetic factors regulate mechanisms of resistance to each stress in this plant species. No correlation was observed between proton resistance and Al resistance among 260 accessions indicating that there is no simple relationship between the genetic factors controlling each trait. Several accessions with different combinations of proton (pH 4.7) and Al (4 μM Al at pH 5.0) resistances were identified by phenotypic cluster analysis. Although this grouping was performed using root growth data, the degree of resistance was correlated with their sensitivity to short-term damage in the root tip, indicating that the same resistance mechanism controls proton resistance at different time scales. Resistant accessions grew better than sensitive ones in acid soil culture. This suggests that proton resistance in hydroponic conditions could be an important index in breeding programs to improve productivity in acid soil, at least in acid sensitive plant species.

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Acknowledgments

This research was supported by RITE and JSPS for HK. We wish to thank to Dr. Thomas Kinraide at ARS, USDA for critical reading of the MS.

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Affiliations

  1. Laboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University, Gifu, Gifu, 501-1193, Japan

    Takashi Ikka, Yuriko Kobayashi & Hiroyuki Koyama

  2. Experimental Plant Division, Biological Resource Center, RIKEN Tsukuba Institute, Tsukuba, Ibaraki, 305-0074, Japan

    Satoshi Iuchi & Masatomo Kobayashi

  3. Laboratory of Genome Biotechnology, Kazusa DNA Research Institute, Kisarazu, Chiba, 292-0818, Japan

    Nozomu Sakurai & Daisuke Shibata

Authors
  1. Takashi Ikka
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  2. Yuriko Kobayashi
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  3. Satoshi Iuchi
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  4. Nozomu Sakurai
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  5. Daisuke Shibata
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  6. Masatomo Kobayashi
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  7. Hiroyuki Koyama
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Correspondence to Hiroyuki Koyama.

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Communicated by P. Langridge.

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122_2007_602_MOESM1_ESM.xls

Supplementary Table 1 Al tolerance and low pH tolerance of Arabidopsis 260 accessions. Seedlings were grown hydroponically in Al (pH 5.0, 4 mM), low pH (pH 4.7) and control (pH 5.0, zero Al) solutions for 7 d. Relative root length of each condition is shown (%; Al or low pH / control) (XLS 47 kb)

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Ikka, T., Kobayashi, Y., Iuchi, S. et al. Natural variation of Arabidopsis thaliana reveals that aluminum resistance and proton resistance are controlled by different genetic factors. Theor Appl Genet 115, 709–719 (2007). https://doi.org/10.1007/s00122-007-0602-5

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Keywords

  • Acid Soil
  • Recombinant Inbred
  • Major QTLs
  • Recombinant Inbred Population
  • Relative Root Length