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Mapping of five resistance genes to sugar-beet powdery mildew using AFLP and anchored SNP markers

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Abstract

Sugar-beet powdery mildew, caused by the fungus Erysiphe betae, now occurs in all sugar-beet growing areas and can reduce sugar yield by up to 30%. Powdery mildew resistant plants from three novel sources were crossed with sugar beet to generate segregating populations. Evaluation of resistance was carried out in artificially inoculated field and controlled environment tests. The resistance level in two of the sources was found to be significantly higher than that in currently available sugar-beet cultivars. AFLP analysis was used in combination with bulked segregant analysis to develop markers linked to the resistant phenotype in each population. Five dominant major resistance genes were identified and assigned the proposed symbols Pm2 to Pm6. Pm3 conferred complete resistance to powdery mildew; the other genes conferred high levels of partial resistance. From the use of anchoring SNP markers, two genes were located to chromosome II and three to chromosome IV. Two of the genes on chromosome IV mapped to the same location and one of the genes on chromosome II mapped to the same region as the previously identified Pm1 gene. With the availability of these genes there is now excellent potential for achieving durable resistance to sugar-beet powdery mildew, thus reducing or obviating the need for chemical control.

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Acknowledgments

This work was supported by the British Beet Research Organisation. We thank Daph Mothersole, Kevin Sawford, Aiming Qi and Steve Hanley for their assistance and Mark Luterbacher and Sally Francis for initial crossing work. Broom’s Barn Research Station is a division of Rothamsted Research and receives grant-aided support from the UK’s Biotechnology and Biological Sciences Research Council.

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  1. Broom’s Barn Research Station, Higham, Bury St Edmunds, IP28 6NP, UK

    M. K. Grimmer, K. M. R. Bean & M. J. C. Asher

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  1. M. K. Grimmer
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  2. K. M. R. Bean
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  3. M. J. C. Asher
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Correspondence to M. K. Grimmer.

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Communicated by C. Gebhardt.

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Grimmer, M.K., Bean, K.M.R. & Asher, M.J.C. Mapping of five resistance genes to sugar-beet powdery mildew using AFLP and anchored SNP markers. Theor Appl Genet 115, 67–75 (2007). https://doi.org/10.1007/s00122-007-0541-1

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  • DOI: https://doi.org/10.1007/s00122-007-0541-1

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