Abstract
Quantitative trait loci (QTL) contributing to the frequency and severity of Ustilago maydis infection in the leaf, ear, stalk, and tassel of maize plants were mapped using an A188 × CMV3 and W23 × CMV3 recombinant inbred (RI) populations. QTLs mapped to genetic bins 2.04 and 9.04–9.05 of the maize genome contributed strongly (R 2 = 18–28%) to variation in the frequency and severity of U. maydis infection over the entire plant in both populations and within the majority of environments. QTLs mapped to bins 3.05, 3.08, and 8.00 in the A188 × CMV3 population and bin 4.05 in both populations significantly contributed to the frequency or severity of infection in only the tassel tissue. QTLs mapped to bin 1.07 in the A188 × CMV3 population and bin 7.00 in the W23 × CMV3 population contributed to U. maydis resistance in only the ear tissue. Interestingly, the CMV3 allele of the QTL mapped to bin 1.10 in the A188 × CMV3 population significantly contributed to U. maydis susceptibility in the ear and stalk but significantly increased resistance in the tassel tissue. Digenic epistatic interactions between the QTL mapped to bin 5.08 and four distinct QTLs significantly contributed to the frequency and severity of infection over the entire plant and within the tassel tissue of the A188 × CMV3 population. Several QTLs detected in this study mapped to regions of the maize genome containing previously mapped U. maydis resistance QTLs and genes involved in plant disease resistance.
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Acknowledgments
The authors would like to acknowledge the help of both the Phillips and May lab in pollinations and scoring U. maydis infection. This project was supported through a NSF Biocomplexity Grant (DMS-0083468 to G. May), a graduate research grant given to Andrew Baumgarten from the University of Minnesota Center for Community Genetics, and support to Ron Phillips from MN Agricultural Experiment Station. Andrew Baumgarten’s work on this project was supported by graduate research assistantship from the Department of Agronomy and Plant Genetics, a fellowship from the University of Minnesota Plant Molecular Genetics Institute, and a University of Minnesota Doctoral Dissertation Fellowship. The authors would specifically like to thank Andy Dosdall for his efforts on this project.
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Communicated by T. Lübberstedt.
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Baumgarten, A.M., Suresh, J., May, G. et al. Mapping QTLs contributing to Ustilago maydis resistance in specific plant tissues of maize. Theor Appl Genet 114, 1229–1238 (2007). https://doi.org/10.1007/s00122-007-0513-5
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DOI: https://doi.org/10.1007/s00122-007-0513-5