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Genetic diversity for aluminum tolerance in sorghum

Theoretical and Applied Genetics volume 114, pages 863–876 (2007)Cite this article

Abstract

Genetic variation for aluminum (Al) tolerance in plants has allowed the development of cultivars that are high yielding on acidic, Al toxic soils. However, knowledge of intraspecific variation for Al tolerance control is needed in order to assess the potential for further Al tolerance improvement. Here we focused on the major sorghum Al tolerance gene, Alt SB , from the highly Al tolerant standard SC283 to investigate the range of genetic diversity for Al tolerance control in sorghum accessions from diverse origins. Two tightly linked STS markers flanking Alt SB were used to study the role of this locus in the segregation for Al tolerance in mapping populations derived from different sources of Al tolerance crossed with a common Al sensitive tester, BR012, as well as to isolate the allelic effects of Alt SB in near-isogenic lines. The results indicated the existence not only of multiple alleles at the Alt SB locus, which conditioned a wide range of tolerance levels, but also of novel sorghum Al tolerance genes. Transgressive segregation was observed in a highly Al tolerant breeding line, indicating that potential exists to exploit the additive or codominant effects of distinct Al tolerance loci. A global, SSR-based, genetic diversity analysis using a broader sorghum set revealed the presence of both multiple Alt SB alleles and different Al tolerance genes within highly related accessions. This suggests that efforts toward broadening the genetic basis for Al tolerance in sorghum may benefit from a detailed analysis of Al tolerance gene diversity within subgroups across a target population.

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Acknowledgments

The authors thank Dr. Fredolino G dos Santos and Dr. José Avelino S Rodrigues for useful suggestions on germplasm selection. We also acknowledge the financial support from The Generation Challenge Programme, the McKnight Foundation Collaborative Crop Research Program, the International Foundation for Science (IFS), and the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG). F.F. Caniato gratefully acknowledges the Fundação Coordenação de Pessoal de Nível Superior (CAPES) for providing financial support for her M.S. program.

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Affiliations

  1. Embrapa Maize and Sorghum, Rod. MG 424, Km 65, 35701-970, Sete Lagoas, MG, Brazil

    F. F. Caniato, C. T. Guimarães, R. E. Schaffert, V. M. C. Alves & J. V. Magalhaes

  2. U.S. Plant Soil and Nutrition Laboratory, USDA-ARS, Cornell University, Ithaca, NY, 14853, USA

    L. V. Kochian

  3. Federal University of Viçosa, Viçosa, MG, 36570-000, Brazil

    A. Borém

  4. Institute for Plant Genomics and Biotechnology and Department of Horticulture, Texas A&M University, College Station, TX, 77843, USA

    P. E. Klein

Authors
  1. F. F. Caniato
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  2. C. T. Guimarães
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  3. R. E. Schaffert
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  4. V. M. C. Alves
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  5. L. V. Kochian
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  6. A. Borém
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  7. P. E. Klein
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  8. J. V. Magalhaes
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Corresponding author

Correspondence to J. V. Magalhaes.

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Communicated by I. Romagosa.

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Table S1: Sorghum accessions evaluated in this study (DOC 106 KB)

Table S2: Standard errors of the means for the data presented in Table 1 (DOC 61 KB)

122_2006_485_MOESM3_ESM.doc

Table S3: Root growth in response to increasing Al3+ activities for the near-isogenic lines (NILs) BR012, 3DX/012, 225/012, and donors 3DX and CMS225. Percent Relative Net Root Growth (RNRG) values are means of three replications (seven plants per replication). RNRG means followed by the same letters within each of the four Al activities are significantly different by the Tukey test (P < 0.05). CV coefficient of variation (DOC 36 KB)

122_2006_485_MOESM4_ESM.doc

Table S4: Percent Relative Net Root Growth (RNRG) means and standard errors of the means for sorghum accessions subjected to {27} µM Al3+, {37} µM Al3+ and {58} µM Al3+ in nutrient solution. Values are the means of three replications for the Al activity of {27} µM Al3+ and two replications for the other Al activities. Seven plants per replication were used for all Al treatments. Accessions whose RNRG means are followed by the same lower-case letters within each of the three Al activities constitute homogeneous RNRG groups by the Scott–Knott test (P < 0.05) (DOC 167 KB)

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Caniato, F.F., Guimarães, C.T., Schaffert, R.E. et al. Genetic diversity for aluminum tolerance in sorghum. Theor Appl Genet 114, 863–876 (2007). https://doi.org/10.1007/s00122-006-0485-x

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Keywords

  • Sorghum
  • Root Growth Inhibition
  • Sorghum Line
  • Sorghum Accession
  • Morphological Race