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An ultradense genetic recombination map for Brassica napus, consisting of 13551 SRAP markers

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Sequence related amplified polymorphism (SRAP) was used to construct an ultradense genetic recombination map for a doubled haploid (DH) population in B. napus. A total of 1,634 primer combinations including 12 fluorescently labeled primers and 442 unlabeled ones produced 13,551 mapped SRAP markers. All these SRAPs were assembled in 1,055 bins that were placed onto 19 linkage groups. Ten of the nineteen linkage groups were assigned to the A genome and the remaining nine to the C genome on the basis of the differential SRAP PCR amplification in two DH lines of B. rapa and B. oleracea. Furthermore, all 19 linkage groups were assigned to their corresponding N1–N19 groups of B. napus by comparison with 55 SSR markers used to construct previous maps in this species. In total, 1,663 crossovers were detected, resulting in a map length span of 1604.8 cM. The marker density is 8.45 SRAPs per cM, and there could be more than one marker in 100 kb physical distance. There are four linkage groups in the A genome with more than 800 SRAP markers each, and three linkage groups in the C genome with more 1,000 SRAP markers each. Our studies suggest that a single SRAP map might be applicable to the three Brassica species, B. napus, B. oleracea and B. rapa. The use of this ultra high-density genetic recombination map in marker development and map-based gene cloning is discussed.

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We thank Dr. C. F. Quiros for his critical reading of this manuscript and providing most primers used in this research. We are indebted to Zixia Niu and Mukhles Rahman for technical assistance. This work was supported by NSERC Industrial Research Chair grant to P. B. E. McVetty and G. Li.

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Correspondence to Genyi Li.

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Communicated by M. Xu.

Electronic supplementary material

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S1. List of uUnlabeled primers used for constructing the high density SRAP map and their sequence (DOC 307 kb)


S2. List of mapped SRAP markers with their sizes, their map position and the primers for producing these SRAP markers (SRAP PCR products were separated with ABI 3100 genetic analyzer (DOC 6.68 mb)

S2-2. (S2 continued) (DOC 7.11 mb)

S2-3. (S2 continued) (DOC 7.10 mb)


S3. Distribution of inferred crossovers on linkage groups and 58 DH lines used for mapping inferred on the basis of 13551 SRAP markers in B. napus. (DOC 353 kb)

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Sun, Z., Wang, Z., Tu, J. et al. An ultradense genetic recombination map for Brassica napus, consisting of 13551 SRAP markers. Theor Appl Genet 114, 1305–1317 (2007).

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