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Simple sequence repeats reveal uneven distribution of genetic diversity in chloroplast genomes of Brassica oleracea L. and (n = 9) wild relatives

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Abstract

Diversity in the chloroplast genome of 171 accessions representing the Brassica ‘C’ (n = 9) genome, including domesticated and wild B. oleracea and nine inter-fertile related wild species, was investigated using six chloroplast SSR (microsatellite) markers. The lack of diversity detected among 105 cultivated and wild accessions of B. oleracea contrasted starkly with that found within its wild relatives. The vast majority of B. oleracea accessions shared a single haplotype, whereas as many as six haplotypes were detected in two wild species, B. villosa Biv. and B. cretica Lam.. The SSRs proved to be highly polymorphic across haplotypes, with calculated genetic diversity values (H) of 0.23–0.87. In total, 23 different haplotypes were detected in C genome species, with an additional five haplotypes detected in B. rapa L. (A genome n = 10) and another in B. nigra L. (B genome, n = 8). The low chloroplast diversity of B. oleracea is not suggestive of multiple domestication events. The predominant B. oleracea haplotype was also common in B. incana Ten. and present in low frequencies in B. villosa, B. macrocarpa Guss, B. rupestris Raf. and B. cretica. The chloroplast SSRs reveal a wealth of diversity within wild Brassica species that will facilitate further evolutionary and phylogeographic studies of this important crop genus.

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Acknowledgments

This work was funded by the UK Biotechnology & Biological Sciences Research Council and the Natural Environment Research Council. The authors would like to thank Dave Pink, Dave Astley and Graham Teakle for advice and provision of accessions and Neale Grant, Sandy McClement and Jason Pole for DNA extraction and plant husbandry.

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Authors and Affiliations

  1. Warwick HRI, Wellesbourne, Warwick, CV35 9EF, UK

    C. J. Allender & J. Lynn

  2. School of Biological Sciences, Plant Science Laboratories, University of Reading, Whiteknights, Reading, RG6 6AS, UK

    J. Allainguillaume

  3. Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK

    G. J. King

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  1. C. J. Allender
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  2. J. Allainguillaume
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  3. J. Lynn
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  4. G. J. King
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Correspondence to C. J. Allender.

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Communicated by H. C. Becker.

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Allender, C.J., Allainguillaume, J., Lynn, J. et al. Simple sequence repeats reveal uneven distribution of genetic diversity in chloroplast genomes of Brassica oleracea L. and (n = 9) wild relatives. Theor Appl Genet 114, 609–618 (2007). https://doi.org/10.1007/s00122-006-0461-5

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  • DOI: https://doi.org/10.1007/s00122-006-0461-5

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