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Development and genetic mapping of sequence-tagged microsatellites (STMs) in bread wheat (Triticum aestivum L.)

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Abstract

The density of SSRs on the published genetic map of bread wheat (Triticum aestivum L.) has steadily increased over the last few years. This has improved the efficiency of marker-assisted breeding and certain types of genetic research by providing more choice in the quality of SSRs and a greater chance of finding polymorphic markers in any cross for a chromosomal region of interest. Increased SSR density on the published wheat genetic map will further enhance breeding and research efforts. Here, sequence-tagged microsatellite profiling (STMP) is demonstrated as a rapid technique for the economical development of anonymous genomic SSRs to increase marker density on the wheat genetic map. A total of 684 polymorphic sequence-tagged microsatellites (STMs) were developed, and 380 were genetically mapped in three mapping populations, with 296 being mapped in the International Triticeae Mapping Initiative W7984 × Opata85 recombinant inbred cross. Across the three populations, a total of 479 STM loci were mapped. Several technological advantages of STMs over conventional SSRs were also observed. These include reduced marker deployment costs for fluorescent-based SSR analysis, and increased genotyping throughput by more efficient electrophoretic separation of STMs and a high amenability to multiplex PCR.

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Acknowledgments

This research was supported by the Grains Research and Development Corporation, Australia, the Value Added Wheat CRC, the Biotechnology and Biological Sciences Research Council, UK and the International Atomic Energy Agency (IAEA RCA-11023).

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Authors and Affiliations

  1. Plant Functional Genomics Center, University of Adelaide, Hartley Grove, Urrbrae, SA, 5064, Australia

    M. J. Hayden & J. Elsden

  2. Molecular Plant Breeding CRC, PMB 1, Glen Osmond, SA, 5064, Australia

    M. J. Hayden

  3. Crop Genetics Department, John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK

    P. Stephenson, A. M. Logojan, C. Rogers, R. M. D. Koebner & J. W. Snape

  4. Plant Breeding Institute, University of Sydney, PMB11, Camden, NSW, 2570, Australia

    D. Khatkar & P. J. Sharp

  5. Value Added Wheat CRC, LB1345, North Ryde, NSW, 2113, Australia

    P. J. Sharp

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  1. M. J. Hayden
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  2. P. Stephenson
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  3. A. M. Logojan
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  7. R. M. D. Koebner
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  8. J. W. Snape
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  9. P. J. Sharp
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Correspondence to M. J. Hayden.

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Communicated by M. Morgante

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Hayden, M.J., Stephenson, P., Logojan, A.M. et al. Development and genetic mapping of sequence-tagged microsatellites (STMs) in bread wheat (Triticum aestivum L.). Theor Appl Genet 113, 1271–1281 (2006). https://doi.org/10.1007/s00122-006-0381-4

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  • DOI: https://doi.org/10.1007/s00122-006-0381-4

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