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Nucleotide diversity in starch synthase IIa and validation of single nucleotide polymorphisms in relation to starch gelatinization temperature and other physicochemical properties in rice (Oryza sativa L.)

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Abstract

The characteristics of starch, such as gelatinization temperature (GT), apparent amylose content (AAC), pasting temperature (PT) and other physicochemical properties, determine the quality of various products of rice, e.g., eating, cooking and processing qualities. The GT of rice flour is controlled by the alk locus, which has been co-mapped to the starch synthase IIa (SSIIa) locus. In this study, we sequenced a 2,051 bp DNA fragment spanning part of intron 6, exon 7, intron 7, exon 8 and part of 3′ untranslated region of SSIIa for 30 rice varieties with diverse geographical distribution and variation in starch physicochemical properties. A total of 24 single nucleotide polymorphisms (SNPs) and one insertion/deletion (InDel) were identified, which could be classified into nine haplotypes. The mean pairwise nucleotide diversity π was 0.00292, and Watterson’s estimator θ was 0.00296 in this collection of rice germplasm. Tajima’s D test for selection showed no significant deviation from the neutral expectation (D = − 0.04612, P > 0.10). However, significant associations were found between seven of the SNPs and peak GT (T p) at P < 0.05, of which two contiguous SNPs (GC/TT) showed a very strong association with T p (P < 0.0001). With some rare exception, this GC/TT polymorphism alone can differentiate rice varieties with high or intermediate GT (possessing the GC allele) from those with low GT (possessing the TT allele). In contrast, none of these SNPs or InDel was significantly associated with amylose content. A further 509 rice varieties with known physicochemical properties (e.g., AAC and PT) and known alleles of other starch synthesizing genes were genotyped for the SSIIa GC/TT alleles. Association analysis indicated that 82% of the total variation of AAC in these samples could be explained by a (CT)n simple sequence repeat (SSR) and a G/T SNP of Waxy gene (Wx), and 62.4% of the total variation of PT could be explained by the GC/TT polymorphism. An additional association analysis was performed between these molecular markers and the thermal and retrogradation properties for a subset of 245 samples from the 509 rice varieties. The SSIIa GC/TT polymorphism explained more than 60% of the total variation in thermal properties, whereas the SSR and SNP of Wx gene explained as much as the SSIIa GC/TT of the total variation in retrogradation properties. Our study provides further support for the utilization of the GC/TT polymorphism in SSIIa. As shown in our study of 509 rice varieties, the GC/TT SNP could differentiate rice with high or intermediate GT from those with low GT in about 90% of cases. Using four primers in a single PCR reaction, the GC/TT polymorphism can be surveyed on a large scale. Thus, this SNP polymorphism can be very useful in marker-assisted selection for the improvement of GT and other physicochemical properties of rice.

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Acknowledgments

This project was supported in part by grants from National Natural Science Foundation of China (30300227), International Atomic Energy Agency (12847/R0) and the Hong Kong Research Grants Council. The authors thank A. M. McClung at USDA-ARS, Rice Research, U.S., Liqing Wei at Chinese Academy of Agricultural Science, Jiankun Xie at Jiangxi Academy of Agricultural Sciences, Guoqing Tu and Jieyun Zhuang at China Rice Research Institute, Qinglong Liu and Junmin Wang at Zhejiang Academy of Agricultural Sciences, Wenyue Chen at Hangzhou Institute of Agricultural Sciences, Xiuru Xu and Honghua Chen at Wenzhou Institute of Agricultural Sciences, and Shengquan Shen at Zhejiang University, for kindly supplying the rice materials.

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  1. Institute of Nuclear Agricultural Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hua Jiachi Campus, Hangzhou, 310029, People’s Republic of China

    J. S. Bao

  2. Department of Botany, The University of Hong Kong, Hong Kong, People’s Republic of China

    H. Corke

  3. Department of Zoology, The University of Hong Kong, Pokfulam Road, Hong Kong, People’s Republic of China

    J. S. Bao & M. Sun

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  1. J. S. Bao
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Correspondence to J. S. Bao or M. Sun.

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Communicated by Q. Zhang

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Bao, J.S., Corke, H. & Sun, M. Nucleotide diversity in starch synthase IIa and validation of single nucleotide polymorphisms in relation to starch gelatinization temperature and other physicochemical properties in rice (Oryza sativa L.). Theor Appl Genet 113, 1171–1183 (2006). https://doi.org/10.1007/s00122-006-0355-6

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