Skip to main content
SpringerLink
Log in

Identification and mapping of Rf-I an inhibitor of the Rf5 restorer gene for Cms-C in maize (Zea mays L.)

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

The restoration of the C-type cytoplasmic male sterility (Cms) has been a common agriculture practice in the production of hybrid seed for many years. In this study, a series of crosses between select sterile and restorer lines, as well as a backcross population of (Cms-C77 × 6233) × 6233, were used to investigate the restoration of C-type Cms. Our results demonstrated that there was an inhibitor of the Rf5 restorer gene. This inhibitor gene, Rf-I, maps to chromosome 7 and is tightly linked with SSR markers, umc2326 and umc2327, at a genetic distance 4.7 and 3.4 cM, respectively. After analyzing our data combined with previous studies, we propose that the restoration of C-type Cms has two dominant genes, Rf4 and Rf5. Rf4 has the ability to restore all genotypes of Cms-C lines; however, there exists an inhibitor for the other restorer gene, Rf5; thus, it can restore only those genotypes of Cms-C lines lacking the Rf-I inhibitor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Beckett JB (1971) Classification of male sterile cytoplasms in maize (Zea mays L.). Crop Sci 11:724–726

    Google Scholar 

  • Chen WC, Luo FH, Ji LY (1979) Some genetic aspects of the C-type cytoplasmic male-sterility in maize and its use in breeding. Acta Agronom Sin 5(4):21–28

    Google Scholar 

  • Duvick DN (1972) Potential usefulness of new cytoplasmic male sterile and sterility system. In: Proceedings of the 27th annual corn and sorghum research conference, pp 197–201

  • Duvick DN, Snyder RJ, Anderson EC (1961) The chromosomal location of Rf1, a restorer gene for cytoplasmic pollen sterile maize. Genetics 46:1245–1252

    PubMed  CAS  Google Scholar 

  • Josephson LM, Morgan TE (1978) Genetics and inheritance of fertility restoration of male sterile cytoplasms in corn. In: Proceedings of the 33rd corn and sorghum research conference 7:13

  • Khey-Pour A, Grace VE, Everett HL (1981) Genetics of fertility restorations in the C-group of cytoplasmic male sterility in maize. Genetics 98:379–388

    Google Scholar 

  • Li JX, Dai JR, Xu QF (1963) Study on the inheritance of cytoplasmic male-sterile and fertility restoration in maize. Acta Agronom Sin 2:339–362

    Google Scholar 

  • Michelmore RW, Paran I, Kesseli V (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic region is by using segregating population. Pro Natl Aca Sci USA 88:9828–9832

    Article  CAS  Google Scholar 

  • Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325

    Article  PubMed  CAS  Google Scholar 

  • Qin TC, Xu ML, Deng DX (1990) Cytoplasmic male-sterility: identification of the number of restorer genes. Maize Genet Coop News Lett 64:124

    Google Scholar 

  • Qin TC, Xu ML, Deng DX, Chen RL, Bian YL (2001) Study on male-sterility in Zea mays VIII. RFLP analysis of mtDNA of YII-1 type maize male-sterile cytoplasm. Acta Agronom Sin 27:185–189

    Google Scholar 

  • Sisco PH (1991) Duplication complicates genetic mapping of Rf4 a restoring gene for cms-C cytoplasmic male sterility in corn. Crop Sci 31:1263–1266

    Article  CAS  Google Scholar 

  • Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 3:739–744

    Article  CAS  Google Scholar 

  • Tang JH, Ji HQ, Huang ZW, Ji LY, Wang CH (2001a) Mapping of the main restorer gene (Rf4) of C-cytoplasmic male sterility in maize with RFLP. J Henan Agric Univ 35:99–102

    CAS  Google Scholar 

  • Tang JH, Liu ZH, Chen WC, Hu YM, Ji LY (2001b) Mapping the main restorer genes of C-cytoplasmic male sterility using SSR method. Acta Agronom Sin 34:592–596

    CAS  Google Scholar 

  • Vidakovic M (1988) Genetics of fertility restoration in cytoplasmic male sterility of the C-type (Cms-c) in maize. Maydica 33:51–64

    Google Scholar 

  • Vidakovic M, Vancetovic J, Vidakovic M (1997a) Complementary genes Rf4, Rf5 and Rf6 are not the unique genetic system for fertility restoration in cmsC of maize (Zea mays L.). Maize Genet Coop News Lett 71:10

    Google Scholar 

  • Vidakovic M, Vancetovic J, Vidakovic M (1997b) The existence of a duplicated or parallel genetic system for fertility restoration in cmsC of maize (Zea mays L.). Maydica 42:313–316

    Google Scholar 

Download references

Acknowledgements

This work was supported by the National High-technology Project of China and National Natural Science Foundation of China.

Author information

Authors and Affiliations

  1. College of Agronomy, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China

    Y. M. Hu, J. H. Tang, H. L. Xie, X. M. Lu, J. H. Niu & W. C. Chen

  2. National Maize Improvement Center of China, China Agricultural University, Beijing, 100094, China

    H. Yang

Authors
  1. Y. M. Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. H. Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. L. Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. X. M. Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. H. Niu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. W. C. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. H. Tang.

Additional information

Communicated by R. Hagemann

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hu, Y.M., Tang, J.H., Yang, H. et al. Identification and mapping of Rf-I an inhibitor of the Rf5 restorer gene for Cms-C in maize (Zea mays L.). Theor Appl Genet 113, 357–360 (2006). https://doi.org/10.1007/s00122-006-0302-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-006-0302-6

Keywords

Search

Navigation