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Single nucleotide polymorphic marker enabling rapid and early screening for the homoeolocus of β-amylase-R1: a gene linked to copper efficiency on 5RL

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Abstract

This study describes the development of a PCR marker to detect the β-amylase-R1 gene of rye. It provides an easy and rapid means for the identification of plants containing the β-amylase-R1. Because rye chromosome segments do not normally recombine with wheat chromosomes, this marker provides a means for tracking all linked genes on that alien 5RL chromosome segment. Reaction conditions were optimised for an annealing temperature of 60°C for a high stringency. The reaction was also optimised for low reaction volumes reducing the cost of the reagents required for the reaction. This PCR test can be used in breeding or mapping programs for the rapid screening of progeny containing translocations of 5RL and hence select for the copper efficiency trait of rye.

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Acknowledgements

The senior author would like to thank Associate Professor K. Shepherd from the University of Adelaide for providing the translocation stocks, addition lines and nullitetrasomic stocks. The CRC Molecular Plant Breeding for financial support, Dr Ian Dundas, Dr Ken Shepherd and Dr Robin Graham from the University of Adelaide for their supervision.

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Authors and Affiliations

  1. Plant and Food Science, School of Agriculture, Food and Wine, The University of Adelaide, PMB 1, Glen Osmond, SA, 5064, Australia

    R. C. Leach & I. S. Dundas

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  1. R. C. Leach
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  2. I. S. Dundas
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Correspondence to R. C. Leach.

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Communicated by M. Morell

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Leach, R.C., Dundas, I.S. Single nucleotide polymorphic marker enabling rapid and early screening for the homoeolocus of β-amylase-R1: a gene linked to copper efficiency on 5RL. Theor Appl Genet 113, 301–307 (2006). https://doi.org/10.1007/s00122-006-0296-0

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  • DOI: https://doi.org/10.1007/s00122-006-0296-0

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