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Molecular cytogenetic characterization of four partial wheat-Thinopyrum ponticum amphiploids and their reactions to Fusarium head blight, tan spot, and Stagonospora nodorum blotch

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Abstract

Four wheat (Triticum aestivum L.)-Thinopyrum ponticum derivatives SS5 (PI604926), SS156 (PI604947), SS363 (PI604970), and SS660 (PI604879), were identified as resistant to Fusarium head blight (FHB), a serious fungal disease of wheat worldwide. Seedling reactions to tan spot and Stagonospora nodorum blotch (SNB), two important foliar diseases of wheat, suggest that these four derivatives are resistant to tan spot and two of them (SS5 and SS156) are resistant to SNB. Fluorescent genomic in situ hybridization (FGISH) patterns of mitotic chromosomes indicate that these four derivatives are partial wheat-Th. ponticum amphiploids, each with a total of 56 chromosomes, though with different amounts of Th. ponticum chromatin. These four amphiploids were hybridized with each other to determine homology between the Th. ponticum genomes in each of the amphiploids. Analysis of chromosome pairing in the F1 hybrids using FGISH suggests that each amphiploid carries a similar set of Th. ponticum chromosomes. These wheat-Th. ponticum amphiploids represent a potential novel source of resistance to FHB, tan spot, and SNB for wheat breeding.

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Acknowledgements

We wish to thank Kay Carlson and Jana Hansen for their invaluable technical assistance. This project was supported by the US Wheat and Barley Scab Initiative and by the North Dakota State Board of Agricultural Research and Education.

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Authors and Affiliations

  1. Department of Plant Sciences, 166 Loftsgard Hall, North Dakota State University (NDSU), Fargo, ND, 58105, USA

    R. E. Oliver & X. Cai

  2. United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Northern Crops Science Laboratory, Fargo, ND, 58105, USA

    S. S. Xu & T. L. Friesen

  3. Department of Plant Pathology, 355 Walster Hall, NDSU, Fargo, ND, 58105, USA

    R. W. Stack

  4. USDA-ARS, Cereal Disease Laboratory, St. Paul, MN, 55108, USA

    Y. Jin

Authors
  1. R. E. Oliver
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  2. S. S. Xu
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  3. R. W. Stack
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  4. T. L. Friesen
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  5. Y. Jin
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  6. X. Cai
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Correspondence to X. Cai.

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Communicated by F. J. Muehlbauer

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Oliver, R.E., Xu, S.S., Stack, R.W. et al. Molecular cytogenetic characterization of four partial wheat-Thinopyrum ponticum amphiploids and their reactions to Fusarium head blight, tan spot, and Stagonospora nodorum blotch. Theor Appl Genet 112, 1473–1479 (2006). https://doi.org/10.1007/s00122-006-0250-1

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  • DOI: https://doi.org/10.1007/s00122-006-0250-1

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