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Linkage map construction in allotetraploid creeping bentgrass (Agrostis stolonifera L.)

Theoretical and Applied Genetics volume 111, pages 795–803 (2005)Cite this article

Abstract

Creeping bentgrass (Agrostis stolonifera L.) is one of the most adapted bentgrass species for use on golf course fairways and putting greens because of its high tolerance to low mowing height. It is a highly outcrossing allotetraploid species (2n=4x=28, A2 and A3 subgenomes). The first linkage map in this species is reported herein, and it was constructed based on a population derived from a cross between two heterozygous clones using 169 RAPD, 180 AFLP, and 39 heterologous cereal and 36 homologous bentgrass cDNA RFLP markers. The linkage map consists of 424 mapped loci covering 1,110 cM in 14 linkage groups, of which seven pairs of homoeologous chromosomes were identified based on duplicated loci. The numbering of all seven linkage groups in the bentgrass map was assigned according to common markers mapped on syntenous chromosomes of ryegrass and wheat. The number of markers linked in coupling and repulsion phase was in a 1:1 ratio, indicating disomic inheritance. This supports a strict allotetraploid inheritance in creeping bentgrass, as suggested by previous work based on chromosomal pairing and isozymes. This linkage map will assist in the tagging and eventually in marker-assisted breeding of economically important quantitative traits like disease resistance to dollar spot (Sclerotinia homoeocarpa F.T. Bennett) and brown patch (Rhizoctonia solani Kuhn).

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Acknowledgements

Our sincere thanks go to Joe Curley for technical assistance. Funding for this project was provided by Hatch Formula Fund (WIS04777) and by the Wisconsin Turfgrass Association.

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Affiliations

  1. Department of Plant Pathology, University of Wisconsin, Madison, WI, 53706, USA

    N. Chakraborty, J. Bae, T. Chang & G. Jung

  2. USDA–ARS US National Arboretum, Washington, DC, 20002, USA

    S. Warnke

Authors
  1. N. Chakraborty
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  2. J. Bae
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  3. S. Warnke
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  4. T. Chang
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  5. G. Jung
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Corresponding author

Correspondence to G. Jung.

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Communicated by P. Langridge

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Chakraborty, N., Bae, J., Warnke, S. et al. Linkage map construction in allotetraploid creeping bentgrass (Agrostis stolonifera L.). Theor Appl Genet 111, 795–803 (2005). https://doi.org/10.1007/s00122-005-2065-x

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  • DOI: https://doi.org/10.1007/s00122-005-2065-x

Keywords

  • Agrostis stolonifera L.
  • Creeping bentgrass
  • Allotetraploid
  • Linkage map
  • Homoeologous chromosomes
  • Molecular markers