Abstract
Cleavage amplified polymorphic sequence (CAPS) markers of strawberry (Fragaria × ananassa Duch.) can be useful for identifying mislabeled or patent-infringing cultivars in the marketplace. However, CAPS markers in octoploid strawberry tend to give unclear bands because multiple homologous sites are simultaneously amplified by the non-selective PCR. To overcome this problem, we used “cluster-specific amplification” based on the nucleotide sequences of PCR products and were able to improve the band clarity of 18 CAPS markers. By analyzing the marker segregation ratio, we demonstrated that 13 clarified markers were derived from single diploid loci that were transmitted to progeny in a manner consistent with Mendelian inheritance. We discuss the genomic structure of octoploid strawberry from the viewpoint of cluster and segregation analysis and suggest that it comprises independent genomes. We tested the utility of all of the markers we developed for cultivar identification and confirmed their ability to distinguish among 64 strawberry cultivars.
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Acknowledgments
We thank the Aichi-Ken Agricultural Research Center, the Agricultural Research Division of the Mie Prefectural Science and Technology Promotion Center, the Gifu Prefectural Agricultural Technology Research Center, the Nara Prefectural Agricultural Experiment Station, the Shizuoka Agricultural Experiment Station, and the Tochigi Prefectural Agriculture Experiment Station for kindly providing plant materials. This study was supported by grants from the National Agricultural Research Organization’s funds for “Integrated Research Program for Functionality and Safety of Food toward Establishment of a Healthy Diet”.
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Kunihisa, M., Fukino, N. & Matsumoto, S. CAPS markers improved by cluster-specific amplification for identification of octoploid strawberry (Fragaria × ananassa Duch.) cultivars, and their disomic inheritance. Theor Appl Genet 110, 1410–1418 (2005). https://doi.org/10.1007/s00122-005-1956-1
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DOI: https://doi.org/10.1007/s00122-005-1956-1